Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1981 Apr 1;195(1):153–157. doi: 10.1042/bj1950153

Chemical, immunological and catalytic properties of 2':3'-cyclic nucleotide 3'-phosphodiesterase purified from brain white matter.

T Kurihara, Y Nishizawa, Y Takahashi, S Odani
PMCID: PMC1162865  PMID: 6272743

Abstract

The amino acid composition, isoelectric point, specificity of the antibody raised and various catalytic properties were determined for 2':3'-cyclic nucleotide 3'-phosphodiesterase (EC 3.1.4.37) purified from bovine brain white matter by a procedure involving solubilization with elastase (EC 3.4.21.11).

Full text

PDF
157

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Drummond R. J., Hamill E. B., Guha A. Purification and comparison of 2',3'-cyclic nucleotide 3'-phosphohydrolases from bovine brain and spinal cord. J Neurochem. 1978 Oct;31(4):871–878. doi: 10.1111/j.1471-4159.1978.tb00122.x. [DOI] [PubMed] [Google Scholar]
  2. Drummond R. J. Purification of 2',3'-cyclic nucleotide 3'-phosphohydrolase from bovine brain by immunoaffinity chromatography: further biochemical characterization of the protein. J Neurochem. 1979 Dec;33(6):1143–1150. doi: 10.1111/j.1471-4159.1979.tb05257.x. [DOI] [PubMed] [Google Scholar]
  3. Guha A., Moore S. Solubilization of 2',3'-cyclic nucleotide 3'-phosphohydrolase from bovine brain without detergents. Brain Res. 1975 May 23;89(2):279–286. doi: 10.1016/0006-8993(75)90719-2. [DOI] [PubMed] [Google Scholar]
  4. Kurihara T., Nishizawa Y., Takahashi Y. The use of non-aqueous chloroform/methanol extraction for the delipidation of brain with minimal loss of enzyme activities. Biochem J. 1977 Jul 1;165(1):135–140. doi: 10.1042/bj1650135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kurihara T., Takahashi Y. Potentiometric and colorimetric methods for the assay of 2',3'-cyclic nucleotide 3'-phosphohydrolase. J Neurochem. 1973 Mar;20(3):719–727. doi: 10.1111/j.1471-4159.1973.tb00032.x. [DOI] [PubMed] [Google Scholar]
  6. Kurihara T., Tsukada Y. The regional and subcellular distribution of 2',3'-cyclic nucleotide 3'-phosphohydrolase in the central nervous system. J Neurochem. 1967 Dec;14(12):1167–1174. doi: 10.1111/j.1471-4159.1967.tb06164.x. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Matsubara H., Sasaki R. M. High recovery of tryptophan from acid hydrolysates of proteins. Biochem Biophys Res Commun. 1969 Apr 29;35(2):175–181. doi: 10.1016/0006-291x(69)90263-0. [DOI] [PubMed] [Google Scholar]
  10. Nishizawa Y., Kurihara T., Takahashi Y. Spectrophotometric assay, solubilization and purification of brain 2':3'-cyclic nucleotide 3'-phosphodiesterase. Biochem J. 1980 Oct 1;191(1):71–82. doi: 10.1042/bj1910071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sprinkle T. J., Grimes M. J., Eller A. G. Isolation of 2',3'-cyclic nucleotide 3'-phosphodiesterase from human brain. J Neurochem. 1980 Apr;34(4):880–887. doi: 10.1111/j.1471-4159.1980.tb09661.x. [DOI] [PubMed] [Google Scholar]
  12. Suda H., Tsukada Y. Improved method for purification of 2',3'-cyclic nucleotide 3'-phosphohydrolase from bovine cerebral white matter. J Neurochem. 1980 Apr;34(4):941–949. doi: 10.1111/j.1471-4159.1980.tb09669.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES