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. 1981 May 1;195(2):435–439. doi: 10.1042/bj1950435

Mitogenicity and binding properties of beta-galactoside-binding lectin from chick-embryo kidney.

M J Pitts, D C Yang
PMCID: PMC1162907  PMID: 7316960

Abstract

THe beta-galactoside-binding lectin binds to glucosamine, mannosamine and galactosamine in addition to beta-galactoside, as determined by the inhibition of haemagglutination. Haemagglutination is further extended to examine the interaction of the binding sites for hexosamines and beta-galactosides, indicating that the binding of hexosamine and beta-galactoside is competitive. The lectin also shows strong mitogenic activity toward lymphocytes from mouse lymph node, as determined by the stimulation of thymidine incorporation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beyer E. C., Zweig S. E., Barondes S. H. Two lactose binding lectins from chicken tissues. Purified lectin from intestine is different from those in liver and muscle. J Biol Chem. 1980 May 10;255(9):4236–4239. [PubMed] [Google Scholar]
  2. Briles E. B., Gregory W., Fletcher P., Kornfeld S. Vertebrate lectins, Comparison of properties of beta-galactoside-binding lectins from tissues of calf and chicken. J Cell Biol. 1979 Jun;81(3):528–537. doi: 10.1083/jcb.81.3.528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Den H., Malinzak D. A. Isolation and properties of beta-D-galactoside-specific lectin from chick embryo thigh muscle. J Biol Chem. 1977 Aug 10;252(15):5444–5448. [PubMed] [Google Scholar]
  4. Hudgin R. L., Pricer W. E., Jr, Ashwell G., Stockert R. J., Morell A. G. The isolation and properties of a rabbit liver binding protein specific for asialoglycoproteins. J Biol Chem. 1974 Sep 10;249(17):5536–5543. [PubMed] [Google Scholar]
  5. Kobiler D., Barondes S. H. Lectin activity from embryonic chick brain, heart, and liver: changes with development. Dev Biol. 1977 Oct 1;60(1):326–330. doi: 10.1016/0012-1606(77)90130-0. [DOI] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Nowak T. P., Kobiler D., Roel L. E., Barondes S. H. Developmentally regulated lectin from embryonic chick pectoral muscle. Purification by affinity chromatography. J Biol Chem. 1977 Sep 10;252(17):6026–6030. [PubMed] [Google Scholar]
  8. Pitts M. J., Yang D. C. Isolation of a developmentally regulated lectin from chick embryo. Biochem Biophys Res Commun. 1980 Jul 31;95(2):750–757. doi: 10.1016/0006-291x(80)90850-5. [DOI] [PubMed] [Google Scholar]
  9. So L. L., Goldstein I. J. Protein-carbohydrate interaction. XX. On the number of combining sites on concanavalin A, the phytohemagglutinin of the jack bean. Biochim Biophys Acta. 1968 Oct 15;165(3):398–404. doi: 10.1016/0304-4165(68)90218-3. [DOI] [PubMed] [Google Scholar]
  10. Teichberg V. I., Silman I., Beitsch D. D., Resheff G. A beta-D-galactoside binding protein from electric organ tissue of Electrophorus electricus. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1383–1387. doi: 10.1073/pnas.72.4.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vesterberg O. Staining of protein zones after isoelectric focusing in polyacrylamide gels. Biochim Biophys Acta. 1971 Aug 27;243(2):345–348. doi: 10.1016/0005-2795(71)90094-8. [DOI] [PubMed] [Google Scholar]
  12. de Waard A., Hickman S., Kornfeld S. Isolation and properties of beta-galactoside binding lectins of calf heart and lung. J Biol Chem. 1976 Dec 10;251(23):7581–7587. [PubMed] [Google Scholar]

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