Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1981 Jun 1;195(3):701–713. doi: 10.1042/bj1950701

The structure of carbohydrate unit B of porcine thyroglobulin.

K Yamamoto, T Tsuji, T Irimura, T Osawa
PMCID: PMC1162943  PMID: 7316980

Abstract

The oligosaccharide fraction was obtained from porcine thyroglobulin by hydrazinolysis. Four fractions of unit B-type oligosaccharides were purified by successive chromatographies on columns of DEAE-cellulose and concanavalin A-Sepharose, and their structures were investigated by the combination of endo- and exo-glycosidase digestions, methylation analysis and Smith degradation. From the results of these studies, the structures of the unit B oligosaccharides were proposed to be as follows: see formula in text. Thus the glycoprotein was found to have triantennary and biantennary complex-type oligosaccharides as acidic sugar chains. Concerning the triantennary oligosaccharides, the following structural features were shown: (1) the sialic acid residues were not localized on certain specific branches but distributed on all three branches; (2) however, alpha (2 leads to 3)-linked sialic acid residues were exclusively located on the terminal of the branch arising from C-4 of the branching alpha-mannose residue, whereas alpha (2 leads to 6)-linked sialic acid residues occupied terminals of the other branches; (3) the outer branching alpha-mannose residue was attached to C-3 or C-6 of an inner branching beta-linked mannose residue, and both types were observed to exist.

Full text

PDF
713

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arima T., Spiro M. J., Spiro R. G. Studies on the carbohydrate units of thyroglobulin. Evaluation of their microheterogeneity in the human and calf proteins. J Biol Chem. 1972 Mar 25;247(6):1825–1835. [PubMed] [Google Scholar]
  2. Baenziger J. U., Fiete D. Structure of the complex oligosaccharides of fetuin. J Biol Chem. 1979 Feb 10;254(3):789–795. [PubMed] [Google Scholar]
  3. Fukuda M., Egami F. Isolation and fractionation of glycopeptides from porcine thyroglobulin. Biochem J. 1971 Jul;123(3):407–414. doi: 10.1042/bj1230407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fukuda M., Kondo T., Osawa T. Studies on the hydrazinolysis of glycoproteins. Core structures of oligosaccharides obtained from Porcine thyroglobulin and pineapple stem bromelain. J Biochem. 1976 Dec;80(6):1223–1232. doi: 10.1093/oxfordjournals.jbchem.a131393. [DOI] [PubMed] [Google Scholar]
  5. HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
  6. Hodges L. C., Laine R., Chan S. K. Structure of the oligosaccharide chains in human alpha 1-protease inhibitor. J Biol Chem. 1979 Sep 10;254(17):8208–8212. [PubMed] [Google Scholar]
  7. Irimura T., Tsuji T., Tagami S., Yamamoto K., Osawa T. Structure of a complex-type sugar chain of human glycophorin A. Biochemistry. 1981 Feb 3;20(3):560–566. doi: 10.1021/bi00506a018. [DOI] [PubMed] [Google Scholar]
  8. Jourdian G. W., Dean L., Roseman S. The sialic acids. XI. A periodate-resorcinol method for the quantitative estimation of free sialic acids and their glycosides. J Biol Chem. 1971 Jan 25;246(2):430–435. [PubMed] [Google Scholar]
  9. Kondo T., Fukuda M., Osawa T. The structure of unit B-type glycopeptides from porcine thyroglobulin. Carbohydr Res. 1977 Oct;58(2):405–414. doi: 10.1016/s0008-6215(00)84367-0. [DOI] [PubMed] [Google Scholar]
  10. Kornfeld R., Kornfeld S. Comparative aspects of glycoprotein structure. Annu Rev Biochem. 1976;45:217–237. doi: 10.1146/annurev.bi.45.070176.001245. [DOI] [PubMed] [Google Scholar]
  11. Krusius T., Finne J., Rauvala H. The structural basis of the different affinities of two types of acidic N-glycosidic glycopeptides for concanavalin A--sepharose. FEBS Lett. 1976 Nov 15;72(1):117–120. doi: 10.1016/0014-5793(76)80911-8. [DOI] [PubMed] [Google Scholar]
  12. Liang C. J., Yamashita K., Muellenberg C. G., Shichi H., Kobata A. Structure of the carbohydrate moieties of bovine rhodopsin. J Biol Chem. 1979 Jul 25;254(14):6414–6418. [PubMed] [Google Scholar]
  13. Matsumoto I., Seno N., Golovtchenko-Matsumoto A. M., Osawa T. Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. J Biochem. 1980 Feb;87(2):535–540. doi: 10.1093/oxfordjournals.jbchem.a132775. [DOI] [PubMed] [Google Scholar]
  14. Mega T., Lujan E., Yoshida A. Studies on the oligosaccharide chains of human alpha 1-protease inhibitor. II. Structure of oligosaccharides. J Biol Chem. 1980 May 10;255(9):4057–4061. [PubMed] [Google Scholar]
  15. Nilsson B., Nordén N. E., Svensson S. Structural studies on the carbohydrate portion of fetuin. J Biol Chem. 1979 Jun 10;254(11):4545–4553. [PubMed] [Google Scholar]
  16. Ogata S., Muramatsu T., Kobata A. Fractionation of glycopeptides by affinity column chromatography on concanavalin A-sepharose. J Biochem. 1975 Oct;78(4):687–696. doi: 10.1093/oxfordjournals.jbchem.a130956. [DOI] [PubMed] [Google Scholar]
  17. Reading C. L., Penhoet E. E., Ballou C. E. Carbohydrate structure of vesicular stomatitis virus glycoprotein. J Biol Chem. 1978 Aug 25;253(16):5600–5612. [PubMed] [Google Scholar]
  18. Stellner K., Saito H., Hakomori S. I. Determination of aminosugar linkages in glycolipids by methylation. Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen. Arch Biochem Biophys. 1973 Apr;155(2):464–472. doi: 10.1016/0003-9861(73)90138-0. [DOI] [PubMed] [Google Scholar]
  19. Tai T., Yamashita K., Ogata-Arakawa M., Koide N., Muramatsu T., Iwashita S., Inoue Y., Kobata A. Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity. J Biol Chem. 1975 Nov 10;250(21):8569–8575. [PubMed] [Google Scholar]
  20. Tsuji T., Irimura T., Osawa T. The carbohydrate moiety of band-3 glycoprotein of human erythrocyte membranes. Biochem J. 1980 Jun 1;187(3):677–686. doi: 10.1042/bj1870677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tsuji T., Yamamoto K., Irimura T., Osawa T. Structure of carbohydrate unit A or porcine thyroglobulin. Biochem J. 1981 Jun 1;195(3):691–699. doi: 10.1042/bj1950691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. UI N., TARUTANI O. Purification of hog thyroglobulin. J Biochem. 1961 Dec;50:508–518. doi: 10.1093/oxfordjournals.jbchem.a127483. [DOI] [PubMed] [Google Scholar]
  23. Yu R. K., Ledeen R. W. Gas--liquid chromatographic assay of lipid-bound sialic acids: measurement of gangliosides in brain of several species. J Lipid Res. 1970 Nov;11(6):506–516. [PubMed] [Google Scholar]
  24. Zinn A. B., Marshall J. S., Carlson D. M. Carbohydrate structures of thyroxine-binding globulin and their effects on hepatocyte membrane binding. J Biol Chem. 1978 Oct 10;253(19):6768–6773. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES