Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1981 Aug 1;197(2):259–268. doi: 10.1042/bj1970259

Bovine aortic chondroitin sulphate- and dermatan sulphate-containing proteoglycans. Isolation, fractionation and chemical characterization.

R Kapoor, C F Phelps, L Cöster, L A Fransson
PMCID: PMC1163122  PMID: 6798960

Abstract

1. Guanidinium chloride (4M) in the presence of proteinase inhibitors extracted 90% of bovine aorta galactosaminoglycans as proteoglycans that were subsequently purified by ion-exchange and gel chromatography. 2. Fractionation of the calcium salts of the purified proteoglycans with increasing concentration of ethanol yielded fractions PG-25 (28%), PG-35 (45%) and PG-50 (37%). 3. Fraction PG-50 contained proteochondroitin 6-sulphate, whereas fractions PG-25 and PG-35 were proteodermatan sulphates of greatly different carbohydrate composition; the molar proportions of L-iduronate-N-acetylgalactosamine 4-sulphate, D-glucuronate-N-acetyl-galactosamine 4-sulphate and D-glucuronate-N-acetylgalactosamine 6-sulphate were 75: 18 :7 in fraction PG-25 and 14 :46 :40 in fraction PG-35. 4. The presence of alternating or mixed sequences with L-iduronate- and D-glucuronate-containing repeating disaccharides was indicated by the formation of tetrasaccharides after chondroitinase AC digestion (single L-iduronate residues) and by the release of fragments containing four or five consecutive D-glucuronate-N-acetylgalactosamine repeats after periodate oxidation and alkaline elimination. 5. The amino acid compositions of fractions PG-25 and PG-35 were similar and markedly different from that of fraction PG-50, which also contained more side chains.

Full text

PDF
259

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelsson I., Heinegård D. Fractionation of proteoglycans from bovine corneal stroma. Biochem J. 1975 Mar;145(3):491–500. doi: 10.1042/bj1450491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  3. BUDDECKE E., KROEZ W., LANKA E. [Chemical composition and macromolecular structure of chondroitin sulfate proteins]. Hoppe Seylers Z Physiol Chem. 1963 Mar;331:196–218. doi: 10.1515/bchm2.1963.331.1.196. [DOI] [PubMed] [Google Scholar]
  4. Bhatti T., Chambers R. E., Clamp J. R. The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides. Biochim Biophys Acta. 1970 Nov 24;222(2):339–347. doi: 10.1016/0304-4165(70)90122-4. [DOI] [PubMed] [Google Scholar]
  5. Cöster L., Fransson L. A. Isolation and characterization of dermatan sulphate proteoglycans from bovine sclera. Biochem J. 1981 Jan 1;193(1):143–153. doi: 10.1042/bj1930143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dobrin P. B. Mechanical properties of arterises. Physiol Rev. 1978 Apr;58(2):397–460. doi: 10.1152/physrev.1978.58.2.397. [DOI] [PubMed] [Google Scholar]
  7. Ehrlich K. C., Radhakrishnamurthy B., Berenson G. S. Isolation of a chondroitin sulfate--dermatan sulfate proteoglycan from bovine aorta. Arch Biochem Biophys. 1975 Nov;171(1):361–369. doi: 10.1016/0003-9861(75)90043-0. [DOI] [PubMed] [Google Scholar]
  8. Eisenstein R., Larsson S. E., Kuettner K. E., Sorgente N., Hascal V. C. The ground substance of the arterial wall. Part 1. Extractability of glycosaminoglycans and the isolation of a proteoglycan from bovine aorta. Atherosclerosis. 1975 Jul-Aug;22(1):1–17. doi: 10.1016/0021-9150(75)90064-7. [DOI] [PubMed] [Google Scholar]
  9. Fransson L. A., Cöster L., Havasmark B., Malmström A., Sjöberg I. The copolymeric structure of pig skin dermatan sulphate. Isolation and characterization of L-idurono-sulphate-containing oligosaccharides from copolymeric chains. Biochem J. 1974 Nov;143(2):379–389. doi: 10.1042/bj1430379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fransson L. A., Cöster L. Interaction between dermatan sulphate chains. II. Structural studies on aggregating glycan chains and oligosaccharides with affinity for dermatan sulphate-substituted agarose. Biochim Biophys Acta. 1979 Jan 4;582(1):132–144. doi: 10.1016/0304-4165(79)90296-4. [DOI] [PubMed] [Google Scholar]
  11. Fransson L. A., Cöster L., Malmstrom A., Sjöberg I. The copolymeric structure of pig skin dermatan suplhate. Characterization of D-glucuronic acid-containing oligosaccharides isolated after controlled degradation of oxydermatan sulphate. Biochem J. 1974 Nov;143(2):369–378. doi: 10.1042/bj1430369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fransson L. A., Havsmark B. Structure of dermatan sulfate. VII. The copolymeric structure of dermatan sulfate from horse aorta. J Biol Chem. 1970 Sep 25;245(18):4770–4783. [PubMed] [Google Scholar]
  13. GARDELL S. Determination of hexosamines. Methods Biochem Anal. 1958;6:289–317. [PubMed] [Google Scholar]
  14. Gardell S., Baker J., Caterson B., Heinegård D., Rodén L. Link protein and a hyaluronic acid-binding region as components of aorta proteoglycan. Biochem Biophys Res Commun. 1980 Aug 29;95(4):1823–1831. doi: 10.1016/s0006-291x(80)80111-2. [DOI] [PubMed] [Google Scholar]
  15. Hascall V. C., Riolo R. L., Hayward J., Jr, Reynolds C. C. Treatment of bovine nasal cartilage proteoglycan with chondroitinases from Flavobacterium heparinum and Proteus vulgaris. J Biol Chem. 1972 Jul 25;247(14):4521–4528. [PubMed] [Google Scholar]
  16. Heinegård D., Axelsson I. Distribution of keratan sulfate in cartilage proteoglycans. J Biol Chem. 1977 Mar 25;252(6):1971–1979. [PubMed] [Google Scholar]
  17. Heinegård D. Extraction, fractionation and characterization of proteoglycans from bovine tracheal cartilage. Biochim Biophys Acta. 1972 Nov 28;285(1):181–192. doi: 10.1016/0005-2795(72)90190-0. [DOI] [PubMed] [Google Scholar]
  18. KAPLAN D., MEYER K. Mucopolysaccharides of aorta at various ages. Proc Soc Exp Biol Med. 1960 Oct;105:78–81. doi: 10.3181/00379727-105-26015. [DOI] [PubMed] [Google Scholar]
  19. KIMMEL J. R., SMITH E. L. Crystalline papain. I. Preparation, specificity, and activation. J Biol Chem. 1954 Apr;207(2):515–531. [PubMed] [Google Scholar]
  20. Kresse H., Heidel H., Buddecke E. Chemical and metabolic heterogeneity of a bovine aorta chondroitin sulfate-dermatan sulfate proteoglycan. Eur J Biochem. 1971 Oct 26;22(4):557–562. doi: 10.1111/j.1432-1033.1971.tb01577.x. [DOI] [PubMed] [Google Scholar]
  21. Kumar V., Berenson G. S., Ruiz H., Dalferes E. R., Jr, Strong J. P. Acid mucopolysaccharides of human aorta. 1. Variations with maturation. J Atheroscler Res. 1967 Sep-Oct;7(5):573–581. doi: 10.1016/s0368-1319(67)80035-8. [DOI] [PubMed] [Google Scholar]
  22. Lindahl U., Bäckström G., Jansson L., Hallén A. Biosynthesis of heparin. II. Formation of sulfamino groups. J Biol Chem. 1973 Oct 25;248(20):7234–7241. [PubMed] [Google Scholar]
  23. Lohmander L. S., De Luca S., Nilsson B., Hascall V. C., Caputo C. B., Kimura J. H., Heinegard D. Oligosaccharides on proteoglycans from the swarm rat chondrosarcoma. J Biol Chem. 1980 Jul 10;255(13):6084–6091. [PubMed] [Google Scholar]
  24. Madhavan M., Chandra K. Acid mucopolysaccharide distribution in normal human aorta in the various anatomic segments. Indian Heart J. 1971 Jul;23(3):202–204. [PubMed] [Google Scholar]
  25. McMurtrey J., Radhakrishnamurthy B., Dalferes E. R., Jr, Berenson G. S., Gregory J. D. Isolation of proteoglycan-hyaluronate complexes from bovine aorta. J Biol Chem. 1979 Mar 10;254(5):1621–1626. [PubMed] [Google Scholar]
  26. Neville D. M., Jr Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem. 1971 Oct 25;246(20):6328–6334. [PubMed] [Google Scholar]
  27. Nozaki Y. The preparation of guanidine hydrochloride. Methods Enzymol. 1972;26:43–50. doi: 10.1016/s0076-6879(72)26005-0. [DOI] [PubMed] [Google Scholar]
  28. Oegema T. R., Jr, Hascall V. C., Dziewiatkowski D. D. Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma. J Biol Chem. 1975 Aug 10;250(15):6151–6159. [PubMed] [Google Scholar]
  29. Oegema T. R., Jr, Hascall V. C., Eisenstein R. Characterization of bovine aorta proteoglycan extracted with guanidine hydrochloride in the presence of protease inhibitors. J Biol Chem. 1979 Feb 25;254(4):1312–1318. [PubMed] [Google Scholar]
  30. REISSIG J. L., STORMINGER J. L., LELOIR L. F. A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem. 1955 Dec;217(2):959–966. [PubMed] [Google Scholar]
  31. Radhakrishnamurthy B., Ruiz H. A., Jr, Berenson G. S. Isolation and characterization of proteoglycans from bovine aorta. J Biol Chem. 1977 Jul 25;252(14):4831–4841. [PubMed] [Google Scholar]
  32. SCOTT J. E. Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues. Methods Biochem Anal. 1960;8:145–197. doi: 10.1002/9780470110249.ch4. [DOI] [PubMed] [Google Scholar]
  33. SIREK O. V., SCHILLER S., DORFMAN A. ACID MUCOPOLYSACCHARIDES IN AORTIC TISSUE OF THE DOG. Biochim Biophys Acta. 1964 Jul 7;83:148–151. doi: 10.1016/0926-6526(64)90030-8. [DOI] [PubMed] [Google Scholar]
  34. Sumner D. S., Hokanson D. E., Strandness D. E., Jr Arterial walls before and after endarterectomy. Stress-strain characteristics and collagen-elastin content. Arch Surg. 1969 Nov;99(5):606–611. doi: 10.1001/archsurg.1969.01340170058013. [DOI] [PubMed] [Google Scholar]
  35. Terho T. T., Hartiala K. Method for determination of the sulfate content of glycosaminoglycans. Anal Biochem. 1971 Jun;41(2):471–476. doi: 10.1016/0003-2697(71)90167-9. [DOI] [PubMed] [Google Scholar]
  36. Yamagata T., Saito H., Habuchi O., Suzuki S. Purification and properties of bacterial chondroitinases and chondrosulfatases. J Biol Chem. 1968 Apr 10;243(7):1523–1535. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES