Abstract
The kinetics of biliary excretion of the main two photoproducts after injection into Gunn rats were examined. The photoproducts that are obtained from experiments in vitro consist of unknown pigment, photobilirubin IXa and a small amount of (ZZ)-bilirubin IXa. It was confirmed previously that the first two photoproducts are identical with the main two photoproducts obtained in vivo. In experiments on four animals, the average of total biliary recoveries of unknown pigment was 81.4%, and that of photobilirubin IXa in the bile estimated by the Sigma-minus method was 29.8 min and that for unknown pigment was 4.3 min. The rate of thermal reversion of photobilirubin IXa to (ZZ)-bilirubin IXa in the bile at 37 degrees C was very rapid, i.e. its half-life was 6.2 min.
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Selected References
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- Cohen A. N., Ostrow J. D. New concepts in phototherapy: photoisomerization of bilirubin IX alpha and potential toxic effects of light. Pediatrics. 1980 Apr;65(4):740–750. [PubMed] [Google Scholar]
- Cummings A. J., King M. L., Martin B. K. A kinetic study of drug elimination: the excretion of paracetamol and its metabolites in man. Br J Pharmacol Chemother. 1967 Feb;29(2):150–157. doi: 10.1111/j.1476-5381.1967.tb01948.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isobe K., Onishi S. Kinetics of the photochemical interconversion among geometric photoisomers of bilirubin. Biochem J. 1981 Mar 1;193(3):1029–1031. doi: 10.1042/bj1931029. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lightner D. A., Wooldridge T. A., McDonagh A. F. Configurational isomerization of bilirubin and the mechanism of jaundice phototherapy. Biochem Biophys Res Commun. 1979 Jan 30;86(2):235–243. doi: 10.1016/0006-291x(79)90857-x. [DOI] [PubMed] [Google Scholar]
- Lightner D. A., Wooldridge T. A., McDonagh A. F. Photobilirubin: an early bilirubin photoproduct detected by absorbance difference spectroscopy. Proc Natl Acad Sci U S A. 1979 Jan;76(1):29–32. doi: 10.1073/pnas.76.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McDonagh A. F., Palma L. A., Lightner D. A. Blue light and bilirubin excretion. Science. 1980 Apr 11;208(4440):145–151. doi: 10.1126/science.7361112. [DOI] [PubMed] [Google Scholar]
- McDonagh A. F., Ramonas L. M. Jaundice phototherapy: micro flow-cell photometry reveals rapid biliary response of Gunn rats to light. Science. 1978 Sep 1;201(4358):829–831. doi: 10.1126/science.581101. [DOI] [PubMed] [Google Scholar]
- Onishi S., Isobe K., Itoh S., Kawade N., Sugiyama S. Demonstration of a geometric isomer of bilirubin-IX alpha in the serum of a hyperbilirubinaemic newborn infant and the mechanism of jaundice phototherapy. Biochem J. 1980 Sep 15;190(3):533–536. doi: 10.1042/bj1900533. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Onishi S., Itoh S., Kawade N., Isobe K., Sugiyama S. The separation of configurational isomers of bilirubin by high pressure liquid chromatography and the mechanism of jaundice phototherapy. Biochem Biophys Res Commun. 1979 Oct 12;90(3):890–896. doi: 10.1016/0006-291x(79)91911-9. [DOI] [PubMed] [Google Scholar]
- Onishi S., Kawade N., Itoh S., Isobe K., Sugiyama S. High-pressure liquid chromatographic analysis of anaerobic photoproducts of bilirubin-IX alpha in vitro and its comparison with photoproducts in vivo. Biochem J. 1980 Sep 15;190(3):527–532. doi: 10.1042/bj1900527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stoll M. S., Zenone E. A., Ostrow J. D., Zarembo J. E. Preparation and properties of bilirubin photoisomers. Biochem J. 1979 Oct 1;183(1):139–146. doi: 10.1042/bj1830139. [DOI] [PMC free article] [PubMed] [Google Scholar]