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. 1981 Nov 15;200(2):365–372. doi: 10.1042/bj2000365

Hydrogenosomes in the rumen protozoon Dasytricha ruminantium Schuberg.

N Yarlett, A C Hann, D Lloyd, A Williams
PMCID: PMC1163544  PMID: 6803778

Abstract

This paper reports for the first time the presence in the anaerobic rumen ciliate Dasytricha ruminantium (Schuberg) of microbody-like organelles, about 0.5 micrometer diameter, with a granular matrix and an equilibrium density of approx. 1.18 g/ml. These organelles can be isolated in a fraction sedimented at 10(5) g-min that contains 67% of the total pyruvate synthase (EC 1.2.7.1), 66% of the hydrogenase (EC 1.18.3.1) and 20% of the lactate dehydrogenase (EC 1.1.1.27). Thus in several respects this fraction is enzymically similar to those containing hydrogenosomes in some other parasitic anaerobic protozoa (the trichomonads). However, in contrast with the hydrogenosomes of trichomonads, the oxygen-tolerant enzyme malate dehydrogenase (decarboxylating) (EC 1.1.1.40) is not particulate, but occurs only in the cytosol. These results enable the proposal of a scheme for the pathway of product formation (acetate, lactate, CO2 and H2) from carbohydrates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAILEY R. W., HOWARD B. H. Preparation of enzymes from rumen protozoa by indole disintegration. Arch Biochem Biophys. 1962 Nov;99:299–303. doi: 10.1016/0003-9861(62)90014-0. [DOI] [PubMed] [Google Scholar]
  2. BRAGG P. D., REEVES R. E. Pathways of glucose dissimilation in Laredo strain of Entamoeba histolytica. Exp Parasitol. 1962 Oct;12:393–400. doi: 10.1016/0014-4894(62)90050-4. [DOI] [PubMed] [Google Scholar]
  3. Bauchop T. Mechanism of hydrogen formation in Trichomonas foetus. J Gen Microbiol. 1971 Sep;68(1):27–33. doi: 10.1099/00221287-68-1-27. [DOI] [PubMed] [Google Scholar]
  4. Baudhuin P., Beaufay H., Rahman-Li Y., Sellinger O. Z., Wattiaux R., Jacques P., De Duve C. Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, D-amino acid oxidase and catalase in rat-liver tissue. Biochem J. 1964 Jul;92(1):179–184. doi: 10.1042/bj0920179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Czerkawski J. W. Methods for determining 2-6-diaminopimelic acid and 2-aminoethylphosphonic acid in gut contents. J Sci Food Agric. 1974 Jan;25(1):45–55. doi: 10.1002/jsfa.2740250106. [DOI] [PubMed] [Google Scholar]
  6. Forsberg C. W., Cheng K. J. The constitutive nature of alkaline phosphatase in rumen bacteria. Can J Microbiol. 1980 Feb;26(2):268–272. doi: 10.1139/m80-043. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Lindmark D. G., Müller M. Biochemical cytology of trichomonad flagellates. II. Subcellular distribution of oxidoreductases and hydrolases in Monocercomonas sp. J Protozool. 1974 May;21(2):374–378. doi: 10.1111/j.1550-7408.1974.tb03673.x. [DOI] [PubMed] [Google Scholar]
  9. Lindmark D. G., Müller M. Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate Tritrichomonas foetus, and its role in pyruvate metabolism. J Biol Chem. 1973 Nov 25;248(22):7724–7728. [PubMed] [Google Scholar]
  10. McCord J. M., Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem. 1969 Nov 25;244(22):6049–6055. [PubMed] [Google Scholar]
  11. Prins R. A., Prast E. R. Oxidation of NADH in a coupled oxidase-peroxidase reaction and its significance for the fermentation in rumen protozoa of the genus Isotricha. J Protozool. 1973 Aug;20(3):471–477. doi: 10.1111/j.1550-7408.1973.tb00929.x. [DOI] [PubMed] [Google Scholar]
  12. Reeves R. E., Warren L. G., Susskind B., Lo H. S. An energy-conserving pyruvate-to-acetate pathway in Entamoeba histolytica. Pyruvate synthase and a new acetate thiokinase. J Biol Chem. 1977 Jan 25;252(2):726–731. [PubMed] [Google Scholar]
  13. Williams A. G., Harfoot C. G. Factors affecting the uptake and metabolism of soluble carbohydrates by the rumen ciliate Dasytricha ruminantium isolated from ovine rumen contents by filtration. J Gen Microbiol. 1976 Sep;96(1):125–136. doi: 10.1099/00221287-96-1-125. [DOI] [PubMed] [Google Scholar]

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