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. 1976 Apr 15;156(1):103–110. doi: 10.1042/bj1560103

Lipid composition of lymphocyte plasma membrane from pig mesenteric lymph node.

G M Levis, G P Evangelatos, M J Crumpton
PMCID: PMC1163721  PMID: 942395

Abstract

1. The lipid fraction of the plasma membrane of pig mesenteric lymph-node lymphocytes contained primarily (94%) neutral lipids and phospholipids in about equal weights. The remianing lipid comprised glycosphingolipids (1.8%), gangliosides (o.27%)and probably ceramides (1.3%).The major phospholipid was phosphatidylcholine (46% of the total), and mono- and tri-hexosylceramides accounted for 72% of the glycosphingolipids. Haematoside was distributed between the glycosphingolipid and ganglioside fractions. The major ganglioside was monosialoganglioside. About 90% of the sialic acid was N-glycollylated. 2. A comparision of the lipid composition of the plasma-membrane fraction with that of the initial lymph-node homogenate showed that the purified membrane contained increased proportions of phospholipids, especially sphingomyelin, glycosphingolipids and gangliosides. 3. Fatty acid analyses showed that the membrane phosphatidylcholine was rich in palmitic acid, that the sphingomeyelin and phosphatidylethanolamine were high in myristic acid and that the glycosphingolipids were rich in oleic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allan D., Auger J., Crumpton M. J. Interaction of phytohemagglutinin with plasma membranes of pig lymphocytes and thymus cells. Exp Cell Res. 1971 Jun;66(2):362–368. doi: 10.1016/0014-4827(71)90689-6. [DOI] [PubMed] [Google Scholar]
  2. Allan D., Crumpton M. J. Preparation and characterization of the plasma membrane of pig lymphocytes. Biochem J. 1970 Nov;120(1):133–143. doi: 10.1042/bj1200133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  4. Carter H. E., Gaver R. C. Improved reagent for trimethylsilylation of sphingolipid bases. J Lipid Res. 1967 Jul;8(4):391–395. [PubMed] [Google Scholar]
  5. Chavin S. I., Johnson S. M., Holliman A. Plasma-membrane-associated immunoglobulins and other polypeptides of pig mesenteric node lymphocytes. Biochem J. 1975 Jun;148(3):417–423. doi: 10.1042/bj1480417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Emmelot P., Van Hoeven R. P. Phospholipid unsaturation and plasma membrane organization. Chem Phys Lipids. 1975 May;14(3):236–246. doi: 10.1016/0009-3084(75)90005-5. [DOI] [PubMed] [Google Scholar]
  7. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  8. Forstner G. G., Wherrett J. R. Plasma membrane and mucosal glycosphingolipids in the rat intestine. Biochim Biophys Acta. 1973 Jun 21;306(3):446–459. doi: 10.1016/0005-2760(73)90183-5. [DOI] [PubMed] [Google Scholar]
  9. Gray G. M. Chromatography of lipids. I. An improved chromatographic procedure for the quantitative isolation of the neutral ceramide-containing glycolipids from mammalian tissues. Biochim Biophys Acta. 1967 Dec 5;144(3):511–518. doi: 10.1016/0005-2760(67)90039-2. [DOI] [PubMed] [Google Scholar]
  10. Hakomori S. Glycolipids of tumor cell membrane. Adv Cancer Res. 1973;18:265–315. doi: 10.1016/s0065-230x(08)60755-1. [DOI] [PubMed] [Google Scholar]
  11. Kanfer J. N., Spielvogel C. On the loss of gangliosides by dialysis. J Neurochem. 1973 May;20(5):1483–1485. doi: 10.1111/j.1471-4159.1973.tb00262.x. [DOI] [PubMed] [Google Scholar]
  12. Karli J. N., Levis G. M. Glycosphingolipids of human thyroid. Lipids. 1974 Oct;9(10):819–824. doi: 10.1007/BF02532151. [DOI] [PubMed] [Google Scholar]
  13. Kwiterovich P. O., Jr, Sloan H. R., Fredrickson D. S. Glycolipids and other lipid constituents of normal human liver. J Lipid Res. 1970 Jul;11(4):322–330. [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Ledeen R., Salsman K., Cabrera M. Gangliosides of bovine adrenal medulla. Biochemistry. 1968 Jun;7(6):2287–2295. doi: 10.1021/bi00846a035. [DOI] [PubMed] [Google Scholar]
  16. Levis G. M., Karli J. N., Malamos B. The phospholipids of the thyroid gland. Clin Chim Acta. 1972 Oct;41:335–345. doi: 10.1016/0009-8981(72)90529-3. [DOI] [PubMed] [Google Scholar]
  17. Levis G. M., Kesse-Elias M. Glycosphingolipids of white cells, lymphoid tissue and bone marrow. Lipids. 1974 Sep;9(9):651–657. doi: 10.1007/BF02532170. [DOI] [PubMed] [Google Scholar]
  18. Maddy A. H. The chemical organization of the plasma membrane of animal cells. Int Rev Cytol. 1966;20:1–65. doi: 10.1016/s0074-7696(08)60796-2. [DOI] [PubMed] [Google Scholar]
  19. Mcelhaney R. N., de Gier J., van der Neut-Kok E. C. The effect of alterations in fatty acid composition and cholesterol content on the nonelectrolyte permeability of Acholeplasma laidlawii B cells and derived liposomes. Biochim Biophys Acta. 1973 Mar 16;298(2):500–512. doi: 10.1016/0005-2736(73)90376-3. [DOI] [PubMed] [Google Scholar]
  20. Miras C. J., Mantzos J. D., Levis G. M. The isolation and partial characterization of glycolipids of normal human leucocytes. Biochem J. 1966 Mar;98(3):782–786. doi: 10.1042/bj0980782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Puro K., Keränen A. Fatty acids and sphingosines of bovine-kidney gangliosides. Biochim Biophys Acta. 1969 Oct 28;187(3):393–400. doi: 10.1016/0005-2760(69)90013-7. [DOI] [PubMed] [Google Scholar]
  22. Ray T. K., Skipski V. P., Barclay M., Essner E., Archibald F. M. Lipid composition of rat liver plasma membranes. J Biol Chem. 1969 Oct 25;244(20):5528–5536. [PubMed] [Google Scholar]
  23. Renkonen O., Gahmberg C. G., Simons K., Käriäinen L. The lipids of the plasma membranes and endoplasmic reticulum from cultured baby hamster kidney cells (BHK21). Biochim Biophys Acta. 1972 Jan 17;255(1):66–78. doi: 10.1016/0005-2736(72)90008-9. [DOI] [PubMed] [Google Scholar]
  24. Roth S. A molecular model for cell interactions. Q Rev Biol. 1973 Dec;48(4):541–563. doi: 10.1086/407816. [DOI] [PubMed] [Google Scholar]
  25. SVENNERHOLM L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957 Jun;24(3):604–611. doi: 10.1016/0006-3002(57)90254-8. [DOI] [PubMed] [Google Scholar]
  26. Vance D. E., Sweeley C. C. Quantitative determination of the neutral glycosyl ceramides in human blood. J Lipid Res. 1967 Nov;8(6):621–630. [PubMed] [Google Scholar]
  27. Weinstein D. B., Marsh J. B., Glick M. C., Warren L. Membranes of animal cells. IV. Lipids of the L cell and its surface membrane. J Biol Chem. 1969 Aug 10;244(15):4103–4111. [PubMed] [Google Scholar]
  28. Weinstein D. B., Marsh J. B., Glick M. C., Warren L. Membranes of animal cells. VI. The glycolipids of the L cell and its surface membrane. J Biol Chem. 1970 Aug 10;245(15):3928–3937. [PubMed] [Google Scholar]
  29. Wiegandt H., Bücking H. W. Carbohydrate components of extraneuronal gangliosides from bovine and human spleen, and bovine kidney. Eur J Biochem. 1970 Aug;15(2):287–292. doi: 10.1111/j.1432-1033.1970.tb01006.x. [DOI] [PubMed] [Google Scholar]
  30. Yu R. K., Ledeen R. W. Gangliosides of human, bovine, and rabbit plasma. J Lipid Res. 1972 Sep;13(5):680–686. [PubMed] [Google Scholar]
  31. Yu R. K., Ledeen R. W. Gas--liquid chromatographic assay of lipid-bound sialic acids: measurement of gangliosides in brain of several species. J Lipid Res. 1970 Nov;11(6):506–516. [PubMed] [Google Scholar]
  32. de Kruyff B., de Greef W. J., van Eyk R. V., Demel R. A., van Deenen L. L. The effect of different fatty acid and sterol composition on the erythritol flux through the cell membrane of Acholeplasma laidlawii. Biochim Biophys Acta. 1973 Mar 16;298(2):479–499. doi: 10.1016/0005-2736(73)90375-1. [DOI] [PubMed] [Google Scholar]
  33. van Hoeven R. P., Emmelot P. Studies on plasma membranes. 18. Lipid class composition of plasma membranes isolated from rat and mouse liver and hepatomas. J Membr Biol. 1972;9(2):105–126. [PubMed] [Google Scholar]

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