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. 1976 Aug 1;157(2):495–497. doi: 10.1042/bj1570495

Some properties of a soluble methane mono-oxygenase from Methylococcus capsulatus strain Bath.

J Colby, H Dalton
PMCID: PMC1163879  PMID: 962879

Abstract

Soluble extracts of Methylococcus capsulatus (Bath), obtained by centrifugation of crude extracts at 160000g for 1h, catalyse the NAD(P)H- and O2-dependent disappearance of bromomethane, and also the formation of methanol from methane. Soluble methane mono-oxygenase is not inhibited by chelating agents or by most electron-transport inhibitors, and is a multicomponent enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colby J., Dalton H., Whittenbury R. An improved assay for bacterial methane mono-oxygenase: some properties of the enzyme from Methylomonas methanica. Biochem J. 1975 Nov;151(2):459–462. doi: 10.1042/bj1510459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ferenci T., Strom T., Quayle J. R. Oxidation of carbon monoxide and methane by Pseudomonas methanica. J Gen Microbiol. 1975 Nov;91(1):79–91. doi: 10.1099/00221287-91-1-79. [DOI] [PubMed] [Google Scholar]
  3. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  4. Ribbons D. W. Oxidation of C1 Compounds by Particulate fractions from Methylococcus capsulatus: distribution and properties of methane-dependent reduced nicotinamide adenine dinucleotide oxidase (methane hydroxylase). J Bacteriol. 1975 Jun;122(3):1351–1363. doi: 10.1128/jb.122.3.1351-1363.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Tonge G. M., Harrison D. E., Knowles C. J., Higgins I. J. Properties and partial purification of the methane-oxidising enzyme system from Methylosinus trichosporium. FEBS Lett. 1975 Oct 15;58(1):293–299. doi: 10.1016/0014-5793(75)80282-1. [DOI] [PubMed] [Google Scholar]
  6. Whittenbury R., Phillips K. C., Wilkinson J. F. Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol. 1970 May;61(2):205–218. doi: 10.1099/00221287-61-2-205. [DOI] [PubMed] [Google Scholar]

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