Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1976 Aug 1;157(2):521–524. doi: 10.1042/bj1570521

Absence of binding of pancreatic and urinary kallikreins to alpha 2-macroglobulin.

E Vahtera, U Hamberg
PMCID: PMC1163886  PMID: 61028

Abstract

Pancreatic and urinary kallikreins failed to form the typical serine proteinase complex with alpha2M (alpha2-macroglobulin). Studies were performed to compare this with the binding of trypsin to alpha2M at various molar binding ratios, with the use of Sephadex G-200 gel filtration to separate free and alpha2M-bound enzyme fractions. The subunit conversion was totally absent with pancreatic kallikrein from lhich traces of a binding proteinase had been removed. The lack of binding is believed to be the result of the restricted specificity of the kallikreins.

Full text

PDF
524

Images in this article

522-1PLATE 1
on p.522-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett A. J., Starkey P. M. The interaction of alpha 2-macroglobulin with proteinases. Characteristics and specificity of the reaction, and a hypothesis concerning its molecular mechanism. Biochem J. 1973 Aug;133(4):709–724. doi: 10.1042/bj1330709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beaven V. H., Pierce J. V., Pisano J. J. A sensitive isotopic procedure for the assay of esterase activity: measurement of human urinary kallikrein. Clin Chim Acta. 1971 Mar;32(1):67–73. doi: 10.1016/0009-8981(71)90465-7. [DOI] [PubMed] [Google Scholar]
  3. ERLANGER B. F., KOKOWSKY N., COHEN W. The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys. 1961 Nov;95:271–278. doi: 10.1016/0003-9861(61)90145-x. [DOI] [PubMed] [Google Scholar]
  4. Frénoy J. P., Razafimahaleo E., Bourrillon R. Preparation des 2 -,macroglobulines humaines individuelles. Proprietes chimiques comparees. Clin Chim Acta. 1972 Nov;42(1):51–56. doi: 10.1016/0009-8981(72)90375-0. [DOI] [PubMed] [Google Scholar]
  5. Ganrot P. O. The combining ratio between trypsin and serum alpha-2-macroglobulin. Acta Chem Scand. 1966;20(8):2299–2300. doi: 10.3891/acta.chem.scand.20-2299. [DOI] [PubMed] [Google Scholar]
  6. Gentou C., Yon J., Filitti-Wurmser S. Propriétés structurales et fonctionnelles des complexes entre l'alpha-2-macroglobuline humaine et la trypsine. Bull Soc Chim Biol (Paris) 1968;50(11):2003–2022. [PubMed] [Google Scholar]
  7. Habermann E., Klett W. Reinigung und einige Eigenschaften eines Kallikreins aus Schweineserum. Biochem Z. 1966 Sep 8;346(2):133–158. [PubMed] [Google Scholar]
  8. Hamberg U. G. The relation of the fibrinolytic mechanism to other systems. Influences on the kinin system by proteolysis in plasma. Proc R Soc Lond B Biol Sci. 1969 Jul 1;173(1032):393–406. doi: 10.1098/rspb.1969.0066. [DOI] [PubMed] [Google Scholar]
  9. Hamberg U., Stelwagen P., Ervast H. S. Human alpha-2-macroglobulin, characterization and trypsin binding. Purification methods, trypsin and plasmin complex formation. Eur J Biochem. 1973 Dec 17;40(2):439–451. doi: 10.1111/j.1432-1033.1973.tb03213.x. [DOI] [PubMed] [Google Scholar]
  10. Harpel P. C. Human plasma alpha 2-macroglobulin. An inhibitor of plasma kallikrein. J Exp Med. 1970 Aug 1;132(2):329–352. doi: 10.1084/jem.132.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Harpel P. C. Studies on human plasma alpha 2-macroglobulin-enzyme interactions. Evidence for proteolytic modification of the subunit chain structure. J Exp Med. 1973 Sep 1;138(3):508–521. doi: 10.1084/jem.138.3.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kutzbach C., Schmidt-Kastner G. Kallikrein from pig pancreas. Purification, separation of components A and B, and crystallization. Hoppe Seylers Z Physiol Chem. 1972 Jul;353(7):1099–1106. doi: 10.1515/bchm2.1972.353.2.1099. [DOI] [PubMed] [Google Scholar]
  13. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  14. Werb Z., Burleigh M. C., Barrett A. J., Starkey P. M. The interaction of alpha2-macroglobulin with proteinases. Binding and inhibition of mammalian collagenases and other metal proteinases. Biochem J. 1974 May;139(2):359–368. doi: 10.1042/bj1390359. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES