Abstract
Reduction of the chromophores of mitochondrial NADH-ubiquinone reductase by NADPH reaches only 50% of the extent of reduction by NADH, monitored at 450 nm. This effect is due to autoxidation of an enzyme component at a higher rate than its reduction by NADPH.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Djavadi-Ohaniance L., Hatefi H. Oxidation of NADPH by submitochondrial particles from beef heart in complete absence of transhydrogenase activity from NADPH to NAD. J Biol Chem. 1975 Dec 25;250(24):9397–9403. [PubMed] [Google Scholar]
- Gutman M., Singer T. P. Spectrophotometric observations on the oxidation-reduction cycle of the respiratory chain-linked reduced nicotinamide-adenine dinucleotide dehydrogenase. Biochemistry. 1970 Nov 24;9(24):4750–4758. doi: 10.1021/bi00826a019. [DOI] [PubMed] [Google Scholar]
- HATEFI Y., HAAVIK A. G., GRIFFITHS D. E. Studies on the electron transfer system. XL. Preparation and properties of mitochondrial DPNH-coenzyme Q reductase. J Biol Chem. 1962 May;237:1676–1680. [PubMed] [Google Scholar]
- Hatefi Y., Hanstein W. G. Interactions of reduced and oxidized triphosphopyridine nucleotides with the electron-transport system of bovine heart mitochondria. Biochemistry. 1973 Aug 28;12(18):3515–3522. doi: 10.1021/bi00742a026. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Ragan C. I. The effects of proteolytic digestion by trypsin on the structure and catalytic properties of reduced nicotinamide-adenine dinucleotide dehydrogenase from bovine heart mitochondria. Biochem J. 1976 May 15;156(2):367–374. doi: 10.1042/bj1560367. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ragan C. I., Widger W. R., King T. E. Pyridine nucleotide transhydrogenase activity of soluble cardiac NADH dehydrogenase and particulate NADH-ubiquinone reductase. Biochem Biophys Res Commun. 1974 Oct 8;60(3):894–900. doi: 10.1016/0006-291x(74)90399-4. [DOI] [PubMed] [Google Scholar]
- Rydström J., Hoek J. B., Ernster L. On the oxidation of reduced nicotinamide dinucleotide phosphate by submitochondrial particles from beef heart. Biochim Biophys Acta. 1973 Jun 28;305(3):694–698. doi: 10.1016/0005-2728(73)90095-9. [DOI] [PubMed] [Google Scholar]