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. 1976 Oct 1;159(1):143–147. doi: 10.1042/bj1590143

A method for the determination of hyaluronate in the presence of other glycosaminoglycans and its application to human intervertebral disc.

T E Hardingham, P Adams
PMCID: PMC1164046  PMID: 999631

Abstract

A highly sensitive proteoglycan-binding assay was developed for the detection and determination of small amounts of hyaluronate. Application of this assay to human intervertebral-disc tissue showed that the radial distribution of hyaluronate closely followed the distribution of proteoglycans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANTONOPOULOS C. A., BORELIUS E., GARDELL S., HAMNSTROM B., SCOTT J. E. The precipitation of polyanions by long-chain aliphatic ammonium compounds. IV. Elution in salt solutions of mucopolysaccharide-quaternary ammonium complexes adsorbed on a support. Biochim Biophys Acta. 1961 Dec 9;54:213–226. doi: 10.1016/0006-3002(61)90360-2. [DOI] [PubMed] [Google Scholar]
  2. Adams P., Muir H. Qualitative changes with age of proteoglycans of human lumbar discs. Ann Rheum Dis. 1976 Aug;35(4):289–296. doi: 10.1136/ard.35.4.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CALLANAN M. J., CARROLL W. R., MITCHELL E. R. Physical and chemical properties of protamine from the sperm of salmon (Oncorhynchus tschawytscha). J Biol Chem. 1957 Nov;229(1):279–287. [PubMed] [Google Scholar]
  4. Emes J. H., Pearce R. H. The proteoglycans of the human intervertebral disc. Biochem J. 1975 Mar;145(3):549–556. doi: 10.1042/bj1450549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eyre D. R., Muir H. Types I and II collagens in intervertebral disc. Interchanging radial distributions in annulus fibrosus. Biochem J. 1976 Jul 1;157(1):267–270. doi: 10.1042/bj1570267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gower W. E., Pedrini V. Age-related variations in proteinpolysaccharides from human nucleus pulposus, annulus fibrosus, and costal cartilage. J Bone Joint Surg Am. 1969 Sep;51(6):1154–1162. [PubMed] [Google Scholar]
  7. Hardingham T. E., Muir H. Binding of oligosaccharides of hyaluronic acid to proteoglycans. Biochem J. 1973 Dec;135(4):905–908. doi: 10.1042/bj1350905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hardingham T. E., Muir H. Hyaluronic acid in cartilage and proteoglycan aggregation. Biochem J. 1974 Jun;139(3):565–581. doi: 10.1042/bj1390565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hardingham T. E., Muir H. The specific interaction of hyaluronic acid with cartillage proteoglycans. Biochim Biophys Acta. 1972 Sep 15;279(2):401–405. doi: 10.1016/0304-4165(72)90160-2. [DOI] [PubMed] [Google Scholar]
  10. Hascall V. C., Heinegård D. Aggregation of cartilage proteoglycans. I. The role of hyaluronic acid. J Biol Chem. 1974 Jul 10;249(13):4232–4241. [PubMed] [Google Scholar]
  11. Heinegård D., Gardell S. Studies on protein-polysaccharide complex (proteoglycan) from human nucleus pulposus. I. Isolation and preliminary characterisation. Biochim Biophys Acta. 1967 Oct 9;148(1):164–171. doi: 10.1016/0304-4165(67)90292-9. [DOI] [PubMed] [Google Scholar]
  12. Hopwood J. J., Robinson H. C. Studies on the polydispersity and heterogeneity of cartilage proteoglycans. Identification of 3 proteoglycan structures in bovine nasal cartilage. Biochem J. 1975 Dec;151(3):581–594. doi: 10.1042/bj1510581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laurent T. C., árány E., Carlsson B., Tidare E. Determination of hyaluronic acid in the microgram range. Anal Biochem. 1969 Oct 1;31(1):133–145. doi: 10.1016/0003-2697(69)90250-4. [DOI] [PubMed] [Google Scholar]
  14. Rosenberg L., Hellmann W., Kleinschmidt A. K. Electron microscopic studies of proteoglycan aggregates from bovine articular cartilage. J Biol Chem. 1975 Mar 10;250(5):1877–1883. [PubMed] [Google Scholar]

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