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. 1976 Nov;159(2):317–322. doi: 10.1042/bj1590317

Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.

K Murray, S G Hughes, J S Brown, S A Bruce
PMCID: PMC1164119  PMID: 11780

Abstract

Two endonucleases, AvaI and AvaII, were isolated from Anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage lambda DNA. Neither enzyme has cofactor requirements beyond Mg2+. Endonuclease AvaI makes eight breaks in the phage lambda chromosome at which the 5'-terminal sequence is pPy-C-G-N. AvaII endonuclease cuts phage lambda DNA more extensively, yielding fragments with the 5'-terminal sequence G-T-C-N or G-A-C-N. Neither enzyme generates cohesive ends.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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