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. 1976 Nov 15;160(2):287–294. doi: 10.1042/bj1600287

A new method for the assay of tissue. S-adenosylhomocysteine and S-adenosylmethione. Effect of pyridoxine deficiency on the metabolism of S-adenosylhomocysteine, S-adenosylmethionine and polyamines in rat liver.

T O Eloranta, E O Kajander, A M Raina
PMCID: PMC1164235  PMID: 1008858

Abstract

The hepatic synthesis and accumulation of S-adenosylhomocysteine, S-adenosylmethionine and polyamines were studied in normal and vitamin B-6-deficient male albino rats. A method involving a single chromatography on a phosphocellulose column was developed for the determination of S-adenosylhomocysteine and S-adenosylmethionine from tissue samples. Feeding the rat with pyridoxine-deficient diet for 3 or 6 weeks resulted in a four- to five-fold increase in the concentration of S-adenosylhomocysteine, whereas that of S-adenosylmethionine was only slighly elevated. The concentration of putrescine was decreased to half, that of spermidine was somewhat decreased and that of spermine remained fairly constant. The activities of L-ornithine decarboxylase, S-adenosyl-L-methionine decarboxylase, L-methionine adenosyltransferase and S-adenosyl-L-homocysteine hydrolase were moderately increased. S-Adenosylmethionine decarboxylase showed no requirement for pyridoxal 5'-phosphate. The major effect of pyridoxine deficiency of S-adenosylmethionine metabolism seems to be a block in the utilization of S-adenosylhomocysteine, resulting in the accumulation of this metabolite to a concentration that may inhibit biological methylation reactions.

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Selected References

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  1. Adibi S. A., Modesto T. A., Morse E. L., Amin P. M. Amino acid levels in plasma, liver, and skeletal muscle during protein deprivation. Am J Physiol. 1973 Aug;225(2):408–414. doi: 10.1152/ajplegacy.1973.225.2.408. [DOI] [PubMed] [Google Scholar]
  2. An F., Tarver H. Effects of diet and other factors on methionine adenosyltransferase levels in rat liver. J Nutr. 1967 Jun;92(2):274–280. doi: 10.1093/jn/92.2.274. [DOI] [PubMed] [Google Scholar]
  3. Chou T. C., Talalay P. The mechanism of S-adenosyl-L-methionine synthesis by purified preparations of bakers' yeast. Biochemistry. 1972 Mar 14;11(6):1065–1073. doi: 10.1021/bi00756a019. [DOI] [PubMed] [Google Scholar]
  4. Cohen S. S., Morgan S., Streibel E. The polyamine content of the tRNA of E. coli. Proc Natl Acad Sci U S A. 1969 Oct;64(2):669–676. doi: 10.1073/pnas.64.2.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DE LA HABA G., CANTONI G. L. The enzymatic synthesis of S-adenosyl-L-homocysteine from adenosine and homocysteine. J Biol Chem. 1959 Mar;234(3):603–608. [PubMed] [Google Scholar]
  6. DUERRE J. A. Preparation and properties of S-adenosyl-L-homocysteine, S-adenosyl-L-homocysteine sulfoxide and S-ribosyl-L-homocysteine. Arch Biochem Biophys. 1962 Jan;96:70–76. doi: 10.1016/0003-9861(62)90453-8. [DOI] [PubMed] [Google Scholar]
  7. Eloranta T. O., Mäenpä P. H., Raina A. M. Synthesis of hepatic polyamines, ribonucleic acid and S-adenosylmethionine in normal and oestrogen-treated chicks. Biochem J. 1976 Jan 15;154(1):95–103. doi: 10.1042/bj1540095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Finkelstein J. D., Chalmers F. T. Pyridoxine effects on cystathionine synthase in rat liver. J Nutr. 1970 Apr;100(4):467–469. doi: 10.1093/jn/100.4.467. [DOI] [PubMed] [Google Scholar]
  9. Finkelstein J. D., Kyle W. E., Harris B. J. Methionine metabolism in mammals: regulatory effects of S-adenosylhomocysteine. Arch Biochem Biophys. 1974 Dec;165(2):774–779. doi: 10.1016/0003-9861(74)90306-3. [DOI] [PubMed] [Google Scholar]
  10. Finkelstein J. D. Methionine metabolism in mammals: the biochemical basis for homocystinuria. Metabolism. 1974 Apr;23(4):387–398. doi: 10.1016/0026-0495(74)90057-2. [DOI] [PubMed] [Google Scholar]
  11. Finkelstein J. D., Mudd S. H. Trans-sulfuration in mammals. The methionine-sparing effect of cystine. J Biol Chem. 1967 Mar 10;242(5):873–880. [PubMed] [Google Scholar]
  12. Hannonen P. Decarboxylation of S-adenosyl-L-methionine in vitamin B6 deficiency in rat liver. Acta Chem Scand B. 1976;30(2):121–124. doi: 10.3891/acta.chem.scand.30b-0121. [DOI] [PubMed] [Google Scholar]
  13. Hoffman J. A rapid liquid chromatographic determination of S-adenosylhomocysteine in subgram amounts of tissue. Anal Biochem. 1975 Oct;68(2):522–530. doi: 10.1016/0003-2697(75)90647-8. [DOI] [PubMed] [Google Scholar]
  14. Hölttä E., Raina A. Stimulation of ornithine decarboxylase and nuclear RNA polymerase activity in rat liver by glucagon and dibutyryl cyclic amp. Acta Endocrinol (Copenh) 1973 Aug;73(4):794–800. doi: 10.1530/acta.0.0730794. [DOI] [PubMed] [Google Scholar]
  15. Kajander O., Eloranta T., Raina A. A sensitive isotopic assay method for S-adenosylhomocysteine hydrolase. Some properties of the enzyme from rat liver. Biochim Biophys Acta. 1976 Jul 8;438(2):522–531. doi: 10.1016/0005-2744(76)90268-0. [DOI] [PubMed] [Google Scholar]
  16. Kerr S. J. Competing methyltransferase systems. J Biol Chem. 1972 Jul 10;247(13):4248–4252. [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Lombardini J. B., Talalay P. Formation, functions and regulatory importance of S-adenosyl-L-methionine. Adv Enzyme Regul. 1970;9:349–384. doi: 10.1016/s0065-2571(71)80054-7. [DOI] [PubMed] [Google Scholar]
  19. McKenzie R. M., Gholson R. K. A simple assay for methionine adenosyltransferase using cation exchange paper and liquid scintillation spectrometry. Anal Biochem. 1973 Jun;53(2):384–391. doi: 10.1016/0003-2697(73)90084-5. [DOI] [PubMed] [Google Scholar]
  20. Ohmori S. Biosynthesis of homocysteine sulfinic acid in the vitamin B6-deficient rat. Hoppe Seylers Z Physiol Chem. 1975 Sep;356(9):1369–1373. doi: 10.1515/bchm2.1975.356.2.1369. [DOI] [PubMed] [Google Scholar]
  21. Okada M., Suzuki K. Amino acid metabolism in rats fed a high protein diet without pyridoxine. J Nutr. 1974 Mar;104(3):287–293. doi: 10.1093/jn/104.3.287. [DOI] [PubMed] [Google Scholar]
  22. Pegg A. E., Williams-Ashman H. G. On the role of S-adenosyl-L-methionine in the biosynthesis of spermidine by rat prostate. J Biol Chem. 1969 Feb 25;244(4):682–693. [PubMed] [Google Scholar]
  23. Raina A., Jansen M., Cohen S. S. Polyamines and the accumulation of ribonucleic acid in some polyauxotrophic strains of Escherichia coli. J Bacteriol. 1967 Nov;94(5):1684–1696. doi: 10.1128/jb.94.5.1684-1696.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Raina A., Jänne J. Physiology of the natural polyamines putrescine, spermidine and spermine. Med Biol. 1975 Jun;53(3):121–147. [PubMed] [Google Scholar]
  25. Salvatore F., Utili R., Zappia V., Shapiro S. K. Quantitative analysis of S-adenosylmethionine and S-adenosylhomocysteine in animal tissues. Anal Biochem. 1971 May;41(1):16–28. doi: 10.1016/0003-2697(71)90187-4. [DOI] [PubMed] [Google Scholar]
  26. Seiler N., Knödgen B. Die Umwandlung von Glutaminsäure, Putrescin und Ornithin in die gamma-Aminobuttersäure im Gehirn. Hoppe Seylers Z Physiol Chem. 1971 Jan;352(1):97–105. doi: 10.1515/bchm2.1971.352.1.97. [DOI] [PubMed] [Google Scholar]
  27. Shapiro S. K., Ehninger D. J. Methods for the analysis and preparation of adenosylmethionine and adenosylhomocysteine. Anal Biochem. 1966 May;15(2):323–333. doi: 10.1016/0003-2697(66)90038-8. [DOI] [PubMed] [Google Scholar]
  28. Shin H. K., Linkswiler H. M. Tryptophan and methionine metabolism of adult females as affected by vitamin B-6 deficiency. J Nutr. 1974 Oct;104(10):1348–1355. doi: 10.1093/jn/104.10.1348. [DOI] [PubMed] [Google Scholar]
  29. Sturman J. A., Kremzner L. T. Polyamine biosynthesis and vitamin B6 deficiency. Evidence for pyridoxal phosphate as coenzyme for S-adenosylmethionine decarboxylase. Biochim Biophys Acta. 1974 Nov 4;372(1):162–170. doi: 10.1016/0304-4165(74)90083-x. [DOI] [PubMed] [Google Scholar]
  30. Uerre J. A., Miller C. H. Preparation of L-homocysteine from L-homocysteine thiolactone. Anal Biochem. 1966 Nov;17(2):310–315. doi: 10.1016/0003-2697(66)90209-0. [DOI] [PubMed] [Google Scholar]
  31. Walker R. D., Duerre J. A. S-adenosylhomocysteine metabolism in various species. Can J Biochem. 1975 Mar;53(3):312–319. doi: 10.1139/o75-044. [DOI] [PubMed] [Google Scholar]

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