Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1977 Feb 15;162(2):445–450. doi: 10.1042/bj1620445

Co-ordinate changes in enzymes of fatty acid synthesis, activation and esterification in rabbit mammary gland druing pregnancy and lactation.

V J Short, D N Brindley, R Dils
PMCID: PMC1164618  PMID: 192226

Abstract

1. The activities of fatty acid synthetase, acyl-CoA synthetase, glycerol phosphate acyltransferase and phosphatidate phosphatase were measured in the mammary glands of rabbits from day 16 of pregnancy to day 15 of post partum. 2. There were significant correlations between the increases in activities of these enzymes during this period. This was the case whether the activities were expressed per mg of homogenate protein, per g wet wt. of tissue or per total wet weight of the whole glands. The only exception was the lack of correlation between the activities of fatty acid synthetase and of phosphatidate phosphatase per g wet wt. of tissue. 3. These co-ordinate increases are discussed in relation to the changes which occur in fatty acid metabolism in the mammary gland during pregnancy and lactation.

Full text

PDF
449

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandes R., Zohar Y., Arad R., Shapiro B. Two forms of microsomal palmitoyl-coenzyme A synthetase. Eur J Biochem. 1973 Apr;34(2):329–332. doi: 10.1111/j.1432-1033.1973.tb02763.x. [DOI] [PubMed] [Google Scholar]
  2. Brindley D. N., Bowley M. Drugs affecting the synthesis of glycerides and phospholipids in rat liver. The effects of clofibrate, halofenate, fenfluramine, amphetamine, cinchocaine, chlorpromazine, demethylimipramine, mepyramine and some of their derivatives. Biochem J. 1975 Jun;148(3):461–469. doi: 10.1042/bj1480461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brindley D. N. The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate by the microsomal fraction of guinea-pig intestinal mucosa. Biochem J. 1973 Apr;132(4):707–715. doi: 10.1042/bj1320707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dodds P. F., Brindley D. N., Gurr M. I. The effects of diet on the esterification of glycerol phosphate, dihydroxyacetone phosphate and 2-hexadecylglycerol by homogenates of rat adipose tissue. Biochem J. 1976 Dec 15;160(3):701–706. doi: 10.1042/bj1600701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forsyth I. A., Strong C. R., Dils R. Interactions of insulin, corticosterone and prolactin in promoting milk-fat synthesis by mammary explants from pregnant rabbits. Biochem J. 1972 Oct;129(4):929–935. doi: 10.1042/bj1290929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gul B., Dils R. Enzymic changes in rabbit and rat mammary gland during the lactation cycle. Biochem J. 1969 Apr;112(3):293–301. doi: 10.1042/bj1120293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gumaa K. A., Greenbaum A. L., McLean P. Adaptive changes in satellite systems related to lipogenesis in rat and sheep mammary gland and in adipose tissue. Eur J Biochem. 1973 Apr 2;34(1):188–198. doi: 10.1111/j.1432-1033.1973.tb02745.x. [DOI] [PubMed] [Google Scholar]
  8. Hajra A. K., Seguin E. B., Agranoff B. W. Rapid labeling of mitochondrial lipids by labeled orthophosphate and adenosine triphosphate. J Biol Chem. 1968 Apr 10;243(7):1609–1616. [PubMed] [Google Scholar]
  9. Hartmann P. E., Jones E. A. Variations in the activity of several enzymes in the mammary glands of non-pregnant, pregnant and lactating rabbits. Biochem J. 1970 Feb;116(4):657–661. doi: 10.1042/bj1160657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kuhn N. J., Lowenstein J. M. Lactogenesis in the rat. Changes in metabolic parameters at parturition. Biochem J. 1967 Dec;105(3):995–1002. doi: 10.1042/bj1050995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lamb R. G., Fallon H. J. An enzymatic explanation for dietary induced alterations in hepatic glycerolipid metabolism. Biochim Biophys Acta. 1974 Apr 26;348(1):179–188. doi: 10.1016/0005-2760(74)90104-0. [DOI] [PubMed] [Google Scholar]
  12. Lloyd-Davies K. A., Brindley D. N. Palmitate activation and esterification in microsomal fractions of rat liver. Biochem J. 1975 Oct;152(1):39–49. doi: 10.1042/bj1520039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mangiapane E. H., Lloyd-Davies K. A., Brindley D. N. A study of some enzymes of glycerolipid biosynthesis in rat liver after subtotal hepatectomy. Biochem J. 1973 May;134(1):103–112. doi: 10.1042/bj1340103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mellenberger R. W., Bauman D. E. Metabolic adaptations during lactogenesis. Fatty acid synthesis in rabbit mammary tissue during pregnancy and lactation. Biochem J. 1974 Mar;138(3):373–379. doi: 10.1042/bj1380373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Short V. J., Dils R., Brindley D. N. Enzymes of glycerolipid synthesis in small-intestinal mucosa of foetal and neonatal guinea pigs. Biochem J. 1975 Dec;152(3):675–679. doi: 10.1042/bj1520675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sod-Moriah U. A., Schmidt G. H. Deoxyribonucleic acid content and proliferative activity of rabbit mammary gland epithelial cells. Exp Cell Res. 1968 Mar;49(3):584–597. doi: 10.1016/0014-4827(68)90206-1. [DOI] [PubMed] [Google Scholar]
  17. Speake B. K., Dils R., Mayer R. J. Effect of hormones on lipogenesis in mammary explants taken from rabbits at different stages of pregnancy and lactation. Biochem Soc Trans. 1976;4(2):238–240. doi: 10.1042/bst0040238. [DOI] [PubMed] [Google Scholar]
  18. Speake B. K., Dils R., Mayer R. J. Regulation of enzyme turnover during tissue differention. Studies on the effects of hormones on the turnover of fatty acid synthetase in rabbit mammary gland in organ culture. Biochem J. 1975 May;148(2):309–320. doi: 10.1042/bj1480309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Strong C. R., Dils R. Fatty acid biosynthesis in rabbit mammary gland during pregnancy and early lactation. Biochem J. 1972 Aug;128(5):1303–1309. doi: 10.1042/bj1281303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strong C. R., Forsyth I., Dils R. The effects of hormones on milk-fat synthesis in mammary explants from pseudopregnant rabbits. Biochem J. 1972 Jul;128(3):509–519. doi: 10.1042/bj1280509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sánchez M., Nicholls D. G., Brindley D. N. [The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate in rat liver mitochondria]. Biochem J. 1973 Apr;132(4):697–706. doi: 10.1042/bj1320697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wiegand R. D., Rao G. A., Reiser R. Dietary regulation of fatty acid synthetase and microsomal glycerophosphate acyltransferase activities in rat liver. J Nutr. 1973 Oct;103(10):1414–1424. doi: 10.1093/jn/103.10.1414. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES