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. 1977 Feb 15;162(2):457–459. doi: 10.1042/bj1620457

Long-chain acyl-coenzyme A synthetase activity of spinach chloroplasts is concentrated in the envelope.

P G Roughan, C R Slack
PMCID: PMC1164620  PMID: 849293

Abstract

Purified chloroplasts were disrupted and then fractionated by discontinuous sucrose-density-gradient centrifugation. Envelopes contained long-chain acyl-CoA synthetase at a specific activity 80 times the activity in the lamellae or the stroma. Acetyl-CoA synthetase was concentrated in the stroma, and chlorophyll was confined to the lamellae membranes. Phospholipase D was not detected in any fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker N., Lynen F. Factors involved in fatty acyl CoA desaturation by fungal microsomes. The relative roles of acyl CoA and phospholipids as substrates. Eur J Biochem. 1971 Mar 11;19(2):200–210. doi: 10.1111/j.1432-1033.1971.tb01305.x. [DOI] [PubMed] [Google Scholar]
  3. Dawson R. M., Hemington N. Some properties of purified phospholipase D and especially the effect of amphipathic substances. Biochem J. 1967 Jan;102(1):76–86. doi: 10.1042/bj1020076. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diesperger H., Müller C. R., Sandermann H., Jr Rapid isolation of a plant microsomal fraction by Mg2+--precipitation. FEBS Lett. 1974 Jul 15;43(2):155–158. doi: 10.1016/0014-5793(74)80990-7. [DOI] [PubMed] [Google Scholar]
  5. Douce R., Holtz R. B., Benson A. A. Isolation and properties of the envelope of spinach chloroplasts. J Biol Chem. 1973 Oct 25;248(20):7215–7222. [PubMed] [Google Scholar]
  6. Douce R. Site of biosynthesis of galactolipids in spinach chloroplasts. Science. 1974 Mar 1;183(4127):852–853. doi: 10.1126/science.183.4127.852. [DOI] [PubMed] [Google Scholar]
  7. Galton D. J., Fraser T. R. An improved assay for long-chain acyl-CoA synthetase. Anal Biochem. 1969 Apr 4;28(1):59–64. doi: 10.1016/0003-2697(69)90156-0. [DOI] [PubMed] [Google Scholar]
  8. Joyard J., Douce R. Mise en évidence et rôle des diacylglycerols de l'enveloppe des chloroplastes d'épinard. Biochim Biophys Acta. 1976 Jan 22;424(1):125–131. doi: 10.1016/0005-2760(76)90057-6. [DOI] [PubMed] [Google Scholar]
  9. Mancha M., Stokes G. B., Stumpf P. K. Fat metabolism in higher plants. The determination of acyl-acyl carrier protein and acyl coenzyme A in a complex lipid mixture 1,2. Anal Biochem. 1975 Oct;68(2):600–608. doi: 10.1016/0003-2697(75)90655-7. [DOI] [PubMed] [Google Scholar]
  10. Poincelot R. P. Isolation and lipid composition of spinach chloroplast envelope membranes. Arch Biochem Biophys. 1973 Nov;159(1):134–142. doi: 10.1016/0003-9861(73)90437-2. [DOI] [PubMed] [Google Scholar]
  11. Roughan P. G., Slack C. R., Holland R. High rates of [1-14C]acetate incorporation into the lipid of isolated spinach chloroplasts. Biochem J. 1976 Sep 15;158(3):593–601. doi: 10.1042/bj1580593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rouser G., Kritchevsky G., Simon G., Nelson G. J. Quantitative analysis of brain and spinach leaf lipids employing silicic acid column chromatography and acetone for elution of glycolipids. Lipids. 1967 Jan;2(1):37–40. doi: 10.1007/BF02531998. [DOI] [PubMed] [Google Scholar]
  13. Schacterle G. R., Pollack R. L. A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal Biochem. 1973 Feb;51(2):654–655. doi: 10.1016/0003-2697(73)90523-x. [DOI] [PubMed] [Google Scholar]
  14. Slack C. R., Roughan P. G., Terpstra J. Some properties of a microsomal oleate desaturase from leaves. Biochem J. 1976 Apr 1;155(1):71–80. doi: 10.1042/bj1550071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Slack C. R., Roughan P. G. The kinetics of incorporation in vivo of (14C)acetate and (14C)carbon dioxide into the fatty acids of glycerolipids in developing leaves. Biochem J. 1975 Nov;152(2):217–228. doi: 10.1042/bj1520217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Van Hummel H. C., Hulsebos T. H., Wintermans J. F. Biosynthesis of galactosyl diglycerides by non-green fractions from chloroplasts. Biochim Biophys Acta. 1975 Feb 20;380(2):219–226. doi: 10.1016/0005-2760(75)90008-9. [DOI] [PubMed] [Google Scholar]
  17. Vijay I. K., Stumpf P. K. Fat metabolism in higher plants. XLVI. Nature of the substrate and the product of oleyl coenzyme A desaturase from Carthamus tinctorius. J Biol Chem. 1971 May 10;246(9):2910–2917. [PubMed] [Google Scholar]

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