Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1977 Sep 15;166(3):627–630. doi: 10.1042/bj1660627

Evidence that fatty acid synthesis in the interscapular brown adipose tissue of cold-adapted rats is increased in vivo by insulin by mechanisms involving parallel activation of pyruvate dehydrogenase and acetyl-coenzyme A carboxylase.

J G McCormack, R M Denton
PMCID: PMC1165048  PMID: 23106

Abstract

Plasma insulin concentrations in cold-adapted rats were altered acutely by administration of glucose or anti-insulin serum. Rates of fatty acid synthesis in interscapular brown adipose tissue were determined from the incorporation of 3H from 3H2O into tissue lipid. Rates of synthesis were greatly elevated after glucose administration and markedly decreased after injection with anti-insulin serum. Parallel changes in the initial activities of both acetyl-CoA carboxylase and pyruvate dehydrogenase were observed under these conditions, but no changes in total activities were evident. The results suggest that this tissue is an important site of fatty acid synthesis in the cold-adapted rat and that this feature of the tissue is sensitive to changes in plasma insulin concentrations.

Full text

PDF
627

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey K., Hahn P., Palaty V. Pyruvate dehydrogenase activity in liver and brown fat of the developing rat. Can J Biochem. 1976 Jun;54(6):534–538. doi: 10.1139/o76-078. [DOI] [PubMed] [Google Scholar]
  2. CROFFORD O. B., RENOLD A. E. GLUCOSE UPTAKE BY INCUBATED RAT EPIDIDYMAL ADIPOSE TISSUE. RATE-LIMITING STEPS AND SITE OF INSULIN ACTION. J Biol Chem. 1965 Jan;240:14–21. [PubMed] [Google Scholar]
  3. Denton R. M. Hormonal regulation of fatty acid synthesis in adipose tissue through changes in the activities of pyruvate dehydrogenase (EC 1.2.4.1) and acetyl-CoA carboxylase (EC 6.4.1.2). Proc Nutr Soc. 1975 Dec;34(3):217–224. doi: 10.1079/pns19750042. [DOI] [PubMed] [Google Scholar]
  4. Denton R. M., Randle P. J., Bridges B. J., Cooper R. H., Kerbey A. L., Pask H. T., Severson D. L., Stansbie D., Whitehouse S. Regulation of mammalian pyruvate dehydrogenase. Mol Cell Biochem. 1975 Oct 31;9(1):27–53. doi: 10.1007/BF01731731. [DOI] [PubMed] [Google Scholar]
  5. Denton R., Bridges B., Brownsey R., Evans G., Hughes W., Stansbie D. Regulation of the conversion of glucose into fat in white adipose tissue by insulin [proceedings]. Biochem Soc Trans. 1977;5(4):894–900. doi: 10.1042/bst0050894. [DOI] [PubMed] [Google Scholar]
  6. Fain J. N., Loken S. C. Response of trypsin-treated brown and white fat cells to hormones. Preferential inhibition of insulin action. J Biol Chem. 1969 Jul 10;244(13):3500–3506. [PubMed] [Google Scholar]
  7. Fain J. N., Reed N., Saperstein R. The isolation and metabolism of brown fat cells. J Biol Chem. 1967 Apr 25;242(8):1887–1894. [PubMed] [Google Scholar]
  8. HIMMS-HAGEN J. LIPID METABOLISM IN WARM-ACCLIMATED AND COLD-ACCLIMATED RATS EXPOSED TO COLD. Can J Physiol Pharmacol. 1965 May;43:379–403. doi: 10.1139/y65-039. [DOI] [PubMed] [Google Scholar]
  9. Halestrap A. P., Denton R. M. Hormonal regulation of adipose-tissue acetyl-Coenzyme A carboxylase by changes in the polymeric state of the enzyme. The role of long-chain fatty acyl-Coenzyme A thioesters and citrate. Biochem J. 1974 Aug;142(2):365–377. doi: 10.1042/bj1420365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hennig G., Löffler G., Wieland O. H. Active and inactive forms of pyruvatedehydrogenase in skeletal muscle as related to the metabolic and functional state of the muscle cell. FEBS Lett. 1975 Nov 15;59(2):142–145. doi: 10.1016/0014-5793(75)80361-9. [DOI] [PubMed] [Google Scholar]
  11. Jungas R. L. Fatty acid synthesis in adipose tissue incubated in tritiated water. Biochemistry. 1968 Oct;7(10):3708–3717. doi: 10.1021/bi00850a050. [DOI] [PubMed] [Google Scholar]
  12. Kerbey A. L., Randle P. J., Cooper R. H., Whitehouse S., Pask H. T., Denton R. M. Regulation of pyruvate dehydrogenase in rat heart. Mechanism of regulation of proportions of dephosphorylated and phosphorylated enzyme by oxidation of fatty acids and ketone bodies and of effects of diabetes: role of coenzyme A, acetyl-coenzyme A and reduced and oxidized nicotinamide-adenine dinucleotide. Biochem J. 1976 Feb 15;154(2):327–348. doi: 10.1042/bj1540327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Knight B. L., Myant N. B. A comparison between the effects of cold exposure in vivo and of noradrenaline in vitro on the metabolism of the brown fat of new-born rabbits. Biochem J. 1970 Aug;119(1):103–111. doi: 10.1042/bj1190103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Severson D. L., Denton R. M., Bridges B. J., Randle P. J. Exchangeable and total calcium pools in mitochondria of rat epididymal fat-pads and isolated fat-cells. Role in the regulation of pyruvate dehydrogenase activity. Biochem J. 1976 Jan 15;154(1):209–223. doi: 10.1042/bj1540209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stansbie D., Brownsey R. W., Crettaz M., Denton R. M. Acute effects in vivo of anti-insulin serum on rates of fatty acid synthesis and activities of acetyl-coenzyme A carboxylase and pyruvate dehydrogenase in liver and epididymal adipose tissue of fed rats. Biochem J. 1976 Nov 15;160(2):413–416. doi: 10.1042/bj1600413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stansbie D., Denton R. M., Bridges B. J., Pask H. T., Randle P. J. Regulation of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads. Effects of starvation, alloxan-diabetes and high-fat diet. Biochem J. 1976 Jan 15;154(1):225–236. doi: 10.1042/bj1540225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vinten J., Gliemann J., Osterlind K. Exchange of 3-O-methylglucose in isolated fat cells. Concentration dependence and effect of insulin. J Biol Chem. 1976 Feb 10;251(3):794–800. [PubMed] [Google Scholar]
  18. Volpe J. J., Vagelos P. R. Mechanisms and regulation of biosynthesis of saturated fatty acids. Physiol Rev. 1976 Apr;56(2):339–417. doi: 10.1152/physrev.1976.56.2.339. [DOI] [PubMed] [Google Scholar]
  19. Windmueller H. G., Spaeth A. E. De novo synthesis of fatty acid in perfused rat liver as a determinant of plasma lipoprotein production. Arch Biochem Biophys. 1967 Nov;122(2):362–369. doi: 10.1016/0003-9861(67)90206-8. [DOI] [PubMed] [Google Scholar]
  20. Windmueller H. G., Spaeth A. E. Perfusion in situ with tritium oxide to measure hepatic lipogenesis and lipid secretion. Normal and orotic acid-fed rats. J Biol Chem. 1966 Jun 25;241(12):2891–2899. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES