Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1975 Feb;145(2):373–378. doi: 10.1042/bj1450373

Oxidation of polymines by diamine oxidase from human seminal plasma.

E Hölttä, P Pulkkinen, K Elfving, J Jänne
PMCID: PMC1165227  PMID: 239684

Abstract

1. Diamine oxidase [amine-oxygen oxidoreductase (deaminating)(pyridoxal-containing), EC 1.4.3.6] was purified from human seminal plasma more than 1,700-fold. The enzyme appeared to be homogeneous on polyacrylamide-gel electrophoresis at two different pH values. 2. The general properties of the enzyme were comparable with those described for other diamine oxidases from different mammalian sources. The molecular weight of the enzyme was calculated to be about 182,000. 3. The enzyme had highest affinity for diamines, but polyamines spermidine and spermine were also degraded at concentrations that can be considered physiological in human semen. 3. The possible degradation of spermine by diamine oxidase in human semen in vivo may give rise to the formation of cytotoxic aldehydes that conceivably can influence the motility and survival of the spermatozoa.

Full text

PDF
375

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AARSEN P. N. RAPID SPECTROPHOTOMETRIC MICROMETHOD FOR DETERMINATION OF HISTAMINASE ACTIVITY. Nature. 1964 Dec 19;204:1195–1195. doi: 10.1038/2041195a0. [DOI] [PubMed] [Google Scholar]
  2. Bachrach U. Metabolism and function of spermine and related polyamines. Annu Rev Microbiol. 1970;24:109–134. doi: 10.1146/annurev.mi.24.100170.000545. [DOI] [PubMed] [Google Scholar]
  3. Bradsley W. G., Crabbe M. J., Scott I. V. The amine oxidases of human placenta and pregnancy plasma. Biochem J. 1974 Apr;139(1):169–181. doi: 10.1042/bj1390169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crabbe M. J., Bradsley W. G. The inhibition of human placental diamine oxidase by substrate analogues. Biochem J. 1974 Apr;139(1):183–189. doi: 10.1042/bj1390183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fair W. R., Clark R. B., Wehner N. A correlation of seminal polyamine levels and semen analysis in the human. Fertil Steril. 1972 Jan;23(1):38–42. doi: 10.1016/s0015-0282(16)38707-6. [DOI] [PubMed] [Google Scholar]
  6. Gunther R. E., Glick D. Determination of histaminase activity in histologic samples and its quantitative distribution in intact human placenta and uterus. J Histochem Cytochem. 1967 Aug;15(8):431–435. doi: 10.1177/15.8.431. [DOI] [PubMed] [Google Scholar]
  7. Hampton J. K., Jr, Rider L. J., Goka T. J., Preslock J. P. The histaminase activity of ceruloplasmin. Proc Soc Exp Biol Med. 1972 Dec;141(3):974–977. doi: 10.3181/00379727-141-36913. [DOI] [PubMed] [Google Scholar]
  8. Jänne J., Hölttä E., Haaranen P., Elfving K. Polyamines and polyamine-metabolizing enzyme activities in human semen. Clin Chim Acta. 1973 Nov 15;48(4):393–401. doi: 10.1016/0009-8981(73)90418-x. [DOI] [PubMed] [Google Scholar]
  9. Kimes B. W., Morris D. R. Inhibition of nucleic acid and protein synthesis in Escherichia coli by oxidized polyamines and acrolein. Biochim Biophys Acta. 1971 Jan 1;228(1):235–244. doi: 10.1016/0005-2787(71)90563-6. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. OKUYAMA T., KOBAYASHI Y. Determination of diamine oxidase activity by liquid scintillation counting. Arch Biochem Biophys. 1961 Nov;95:242–250. doi: 10.1016/0003-9861(61)90141-2. [DOI] [PubMed] [Google Scholar]
  12. Raina A., Cohen S. S. Polyamines and RNA synthesis in a polyauxotrophic strain of E. coli. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1587–1593. doi: 10.1073/pnas.55.6.1587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TABOR C. W., ROSENTHAL S. M. Pharmacology of spermine and spermidine; some effects on animals and bacteria. J Pharmacol Exp Ther. 1956 Feb;116(2):139–155. [PubMed] [Google Scholar]
  14. TABOR C. W., TABOR H., BACHRACH U. IDENTIFICATION OF THE AMINOALDEHYDES PRODUCED BY THE OXIDATION OF SPERMINE AND SPERMIDINE WITH PURIFIED PLASMA AMINE OXIDASE. J Biol Chem. 1964 Jul;239:2194–2203. [PubMed] [Google Scholar]
  15. TABOR C. W., TABOR H., ROSENTHAL S. M. Purification of amine oxidase from beef plasma. J Biol Chem. 1954 Jun;208(2):645–661. [PubMed] [Google Scholar]
  16. Tryding N., Willert B. Determination of plasma diamine oxidase (histaminase) in clinical practice. A comparison between a biological method and a radiochemical micromethod. Scand J Clin Lab Invest. 1968;22(1):29–32. doi: 10.3109/00365516809160732. [DOI] [PubMed] [Google Scholar]
  17. WEAVER R. H., HERBST E. J. Metabolism of diamines and polyamines in microorganisms. J Biol Chem. 1958 Apr;231(2):637–646. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES