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. 1975 Feb;146(2):425–437. doi: 10.1042/bj1460425

The synthesis of fatty acids in avocado mesocarp and cauliflower bud tissue.

P J Weaire, R G Kekwick
PMCID: PMC1165321  PMID: 1156380

Abstract

1. Plastid and mitochondrial preparations were obtained by density-gradient centrifugation of homogenates made by gentle disintergration of avocado fruit mesocarp and cauliflower bud tissue. 2. The mitochondrial preparations had respiratory activity but did not incorporate [1-14C]acetate into fatty acids. 3. The plastid preparations incorporated [1--14C]acetate into the range of fatty acids found in the parent tissue. No fatty acid synthetase activity could be detected in the 12000g supernatant of these homogenates. 4. Homogenates produced by rupture of the tissue in an Ato-Mix blender and plastid preparations disintegrated by ultrasonic treatment both had fatty acid synthetase activity which did not sediment at 105000g and which formed mainly [14-C]stearate from [2-14C]malonyl-CoA. 5. It is concluded that the plastids are the principal site of fatty acid biosynthesis in the tissues studied.

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Selected References

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  1. BARRON E. J., SQUIRES C., STUMPF P. K. Fat metabolism in higher plants. XV. Enzymic synthesis of fatty acids by an extract of avocado mesocarp. J Biol Chem. 1961 Oct;236:2610–2614. [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. Baker J. E., Elfvin L. G., Biale J. B., Honda S. I. Studies on ultrastructure and purification of isolated plant mitochondria. Plant Physiol. 1968 Dec;43(12):2001–2022. doi: 10.1104/pp.43.12.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHANCE B., WILLIAMS G. R. The respiratory chain and oxidative phosphorylation. Adv Enzymol Relat Subj Biochem. 1956;17:65–134. doi: 10.1002/9780470122624.ch2. [DOI] [PubMed] [Google Scholar]
  5. Chappell J. B. The oxidation of citrate, isocitrate and cis-aconitate by isolated mitochondria. Biochem J. 1964 Feb;90(2):225–237. doi: 10.1042/bj0900225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Everson R. G., Gibbs M. Photosynthetic assimilation of carbon dioxide and acetate by isolated chloroplasts. Plant Physiol. 1967 Aug;42(8):1153–1154. doi: 10.1104/pp.42.8.1153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Galliard T., Michell R. H., Hawthorne J. N. Incorporation of phosphate into diphosphoinositide by subcellular fractions from liver. Biochim Biophys Acta. 1965 Dec 2;106(3):551–563. doi: 10.1016/0005-2760(65)90071-8. [DOI] [PubMed] [Google Scholar]
  8. Higgins M. J., Kekwick R. G. An investigation into the role of malonyl-coenzyme A in isoprenoid biosynthesis. Biochem J. 1973 May;134(1):295–310. doi: 10.1042/bj1340295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MADSEN J., ABRAHAM S., CHAIKOFF I. L. THE CONVERSION OF GLUTAMATE CARBON TO FATTY ACID CARBON VIA CITRATE. I. THE INFLUENCE OF GLUCOSE IN LACTATING RAT MAMMARY GLAND SLICES. J Biol Chem. 1964 May;239:1305–1309. [PubMed] [Google Scholar]
  10. MUDD J. B., STUMPF P. K. Fat metabolism in higher plants. XIV. Factors affecting the synthesis of oleic acid by particulate preparations from avocado mesocarp. J Biol Chem. 1961 Oct;236:2602–2609. [PubMed] [Google Scholar]
  11. Mazliak P., Oursel A., Abdelkader A. B., Grosbois M. Biosynthèse des acides gras dans les mitochondries végétales isolées. Eur J Biochem. 1972 Jul 24;28(3):399–411. doi: 10.1111/j.1432-1033.1972.tb01926.x. [DOI] [PubMed] [Google Scholar]
  12. OVERATH P., STUMPF P. K. FAT METABOLISM IN HIGHER PLANTS. 23. PROPERTIES OF A SOLUBLE FATTY ACID SYNTHETASE FROM AVOCADO MESOCARP. J Biol Chem. 1964 Dec;239:4103–4110. [PubMed] [Google Scholar]
  13. STUMPF P. K., BARBER G. A. Fat metabolism in higher plants. IX. Enzymic synthesis of long chain fatty acids by avocado particles. J Biol Chem. 1957 Jul;227(1):407–417. [PubMed] [Google Scholar]
  14. STUMPF P. K., JAMES A. T. The biosynthesis of long-chain fatty acids by lettuce chloroplast preparations. Biochim Biophys Acta. 1963 Feb 19;70:20–32. doi: 10.1016/0006-3002(63)90715-7. [DOI] [PubMed] [Google Scholar]
  15. Simoni R. D., Criddle R. S., Stumpf P. K. Fat metabolism in higher plants. XXXI. Purification and properties of plant and bacterial acyl carrier proteins. J Biol Chem. 1967 Feb 25;242(4):573–581. [PubMed] [Google Scholar]
  16. Stumpf P. K., Boardman N. K. Fat metabolism in higher plants. XXXIX. Effect of adenosine triphosphate and triton X-100 on lipid synthesis by isolated spinach chloroplasts. J Biol Chem. 1970 May 25;245(10):2579–2587. [PubMed] [Google Scholar]
  17. YANG S. F., STUMPF P. K. FAT METABOLISM IN HIGHER PLANTS. XXI. BIOSYNTHESIS OF FATTY ACIDS BY AVOCADO MESOCARP ENZYME SYSTEMS. Biochim Biophys Acta. 1965 Feb 1;98:19–26. [PubMed] [Google Scholar]
  18. Zilkey B., Canvin D. T. Subcellular localization of oleic acid biosynthesis enzymes in the developing castor bean endosperm. Biochem Biophys Res Commun. 1969 Mar 10;34(5):646–653. doi: 10.1016/0006-291x(69)90787-6. [DOI] [PubMed] [Google Scholar]

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