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. 1975 Aug;149(2):447–461. doi: 10.1042/bj1490447

Kinetic studies on the hydroxylation of p-coumaric acid to caffeic acid by spinach-beet phenolase.

R J McIntyre, P F Vaughan
PMCID: PMC1165638  PMID: 170916

Abstract

1. A spectrophotometric assay is described that enables the hydroxylation of p-coumaric acid to caffeic acid, catalysed by spinach-beet phenolase, to be followed continuously. 2. Initial-velocity and inhibitor studies indicate that the order of substrate addition is oxygen, p-coumaric acid and electron donor, with an irreversible step separating the binding of each substrate. 3. Caffeic acid is most likely to act as electron donor at the active site; other electron donors, such as ascorbic acid, NADH and dimethyltetrahydropteridine, function mainly to recycle cofactor amounts of caffeic acid. 4. A reaction scheme, consistent with these data, is proposed.

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Selected References

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