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. 1973 Nov;135(3):457–462. doi: 10.1042/bj1350457

N-Acetyl-β-d-hexosaminidase component A. Different forms in human tissues and fluids

J Uzoma Ikonne 1, Roland B Ellis 1
PMCID: PMC1165847  PMID: 4359018

Abstract

1. Hexosaminidase A of human serum was resolved into two components, a minor form with properties identical with those of the single hexosaminidase A component of human liver, and a major form with significantly different properties. 2. The major serum hexosaminidase A form was eluted from a DEAE-cellulose column at a lower salt concentration than that required to elute the liver form. 3. A multiple-pass technique was used to elute the major serum enzyme A from a Sephadex G-150 column before that of liver enzyme A. 4. Clostridium perfringens neuraminidase converted the major component of serum hexosaminidase A into a form that was held less tightly by DEAE-cellulose, but the minor component of the A enzyme of serum, and the A enzyme of liver were not affected. 5. The hexosaminidase A from tears was similar to the A enzyme from serum, whereas those from several human tissues and from urine and lymph were similar to the liver form. 6. The A enzyme from serum may be derived from the A enzyme from liver by glycosylation before secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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