Abstract
Sodium perchlorate in high concentrations will remove from solution the detergent sodium dodecyl sulphate and protein complexed with it. This and the failure of proteins to be precipitated by ethanol from solutions containing a high concentration of sodium perchlorate can be utilized as efficient, rapid and simple deproteinization procedures during the preparation of nucleic acids.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauer W. R. Structure of DNA in denaturing solvents. I. Bacteriophage PM2 DNA in aqueous sodium perchlorate. J Mol Biol. 1972 Jun 20;67(2):183–198. doi: 10.1016/0022-2836(72)90235-5. [DOI] [PubMed] [Google Scholar]
- Kirby K. S., Fox-Carter E., Guest M. Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria. Biochem J. 1967 Jul;104(1):258–262. doi: 10.1042/bj1040258. [DOI] [PMC free article] [PubMed] [Google Scholar]