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. 1973 Oct;136(2):387–393. doi: 10.1042/bj1360387

Cellular reactions of O6-methylguanine, a product of some alkylating carcinogens

Carolyn Thatcher Miller 1, Philip D Lawley 1, Sudhirkumar A Shah 1
PMCID: PMC1165965  PMID: 4590203

Abstract

Cultures of a purine-requiring mutant of Chinese hamster ovary cells (CHO-104b), randomly bred hamster embryo cells, or Escherichia coli Bs−1 were treated with non-toxic doses of 3H-labelled O6-methylguanine. DNA and RNA were isolated and subjected to enzymic digestion to nucleosides at pH8. The products of digestion were analysed by ion-exchange chromatography on columns of Dowex 50 (NH4+ form) at pH8.9. No 3H-labelled O6-methylguanosine was detected in nucleic acid digests. 3H-labelled O6-methylguanine was O-demethylated yielding [3H]guanine in CHO-104b cells. Radioactivity in nucleic acid digests was associated with thymidine, guanosine, deoxyguanosine and an unidentified early-eluting product. Reports of similar unidentified products from nucleic acids labelled with various agents are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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