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. 1974 Mar;138(3):407–413. doi: 10.1042/bj1380407

Rabbit β-glucuronidase. Subcellular distribution and immunochemical properties

Roger T Dean 1,*
PMCID: PMC1166226  PMID: 4215419

Abstract

1. The subcellular distribution of β-glucuronidase and other hydrolases in rabbit liver was investigated. β-Glucuronidase was found in both microsomal and lysosomal fractions. 2. Multiple forms of β-glucuronidase were present in extracts of microsomal and lysosomal fractions. All forms were common to both fractions. 3. A specific antiserum against β-glucuronidase was raised, and characterized by immunoprecipitation and affinity-chromatography procedures. 4. The immunological identity of the multiple forms in the pure β-glucuronidase preparation, and the immunological identity of the β-glucuronidase complement of lysosomal extracts with that of microsomal extracts, were demonstrated by means of the antiserum. The presence of inactive enzyme in various enzyme preparations was shown.

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Selected References

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