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. 1974 Mar;138(3):445–451. doi: 10.1042/bj1380445

Guinea-pig liver tryptophan pyrrolase. Absence of detectable apoenzyme activity and of hormonal induction by cortisol and possible regulation by tryptophan

Abdulla A-B Badawy 1, Myrddin Evans 1
PMCID: PMC1166230  PMID: 4429542

Abstract

1. When assayed in fresh homogenates, guinea-pig liver tryptophan pyrrolase exists only as holoenzyme. It does not respond to agents that activate or inhibit the rat liver enzyme in vitro. Only by aging (for 30min at 5°C) does the guinea-pig enzyme develop a requirement for ascorbate. 2. The guinea-pig liver enzyme is activated by the administration of tryptophan but not cortisol, salicylate, ethanol or 5-aminolaevulinate. 3. The tryptophan enhancement of the guinea-pig liver pyrrolase activity is prevented by 0, 34 and 86% by pretreatment with actinomycin D, cycloheximide or allopurinol respectively. 4. The guinea-pig liver tryptophan pyrrolase is more sensitive to tryptophan administration than is the rat enzyme. On the other hand, the concentrations of tryptophan in sera and livers of guinea pigs are 45–52% less than those in rats. 5. It is suggested that tryptophan may regulate the activity of guinea-pig liver tryptophan pyrrolase by mobilizing a latent form of the enzyme whose primary function is the detoxication of its substrate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARVOLA A., FORSANDER O. Comparison between water and alcohol consumption in six animal species infree choice experiments. Nature. 1961 Aug 19;191:819–820. doi: 10.1038/191819a0. [DOI] [PubMed] [Google Scholar]
  2. Badawy A. A., Evans M. The mechanism of inhibition of rat liver tryptophan pyrrolase activity by 4-hydroxypyrazolo(3,4-d)pyrimidine (Allopurinol). Biochem J. 1973 Jul;133(3):585–591. doi: 10.1042/bj1330585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Badawy A. A., Smith M. J. Changes in liver tryptophan and tryptophan pyrrolase activity after administration of salicylate and tryptophan to the rat. Biochem Pharmacol. 1972 Jan;21(1):97–101. doi: 10.1016/0006-2952(72)90254-7. [DOI] [PubMed] [Google Scholar]
  4. Badawy A. A., Smith M. J. The effects of salicylate on the activity of rat liver tryptophan pyrrolase in vitro and in vivo. Biochem J. 1971 Jun;123(2):171–174. doi: 10.1042/bj1230171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown J. N., Dodgen C. L. Fish liver tryptophan pyrrolase: the apparent absence of both hormonal regulation and substrate induction. Biochim Biophys Acta. 1968 Oct 15;165(3):463–469. doi: 10.1016/0304-4165(68)90226-2. [DOI] [PubMed] [Google Scholar]
  6. CIVEN M., KNOX W. E. The independence of hydrocortisone and tryptophan inductions of tryptophan pyrrolase. J Biol Chem. 1959 Jul;234(7):1787–1790. [PubMed] [Google Scholar]
  7. Cho-Chung Y. S., Pitot H. C. Feedback control of rat liver tryptophan pyrrolase. I. End product inhibition of trytophan pyrrolase activity. J Biol Chem. 1967 Mar 25;242(6):1192–1198. [PubMed] [Google Scholar]
  8. Druyan R., Kelly A. The effect of exogenous -aminolaevulinate on rat liver haem and cytochromes. Biochem J. 1972 Oct;129(5):1095–1099. doi: 10.1042/bj1291095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FEIGELSON P., GREENGARD O. A microsomal iron-porphyrin activator of rat liver tryptophan pyrrolase. J Biol Chem. 1961 Jan;236:153–157. [PubMed] [Google Scholar]
  10. GREENGARD O., FEIGELSON P. The activation and induction of rat liver tryptophan pyrrolase in vivo by its substrate. J Biol Chem. 1961 Jan;236:158–161. [PubMed] [Google Scholar]
  11. GREENGARD O., SMITH M. A., ACS G. Relation of cortisone and synthesis of ribonucleic acid to induced and developmental enzyme formation. J Biol Chem. 1963 Apr;238:1548–1551. [PubMed] [Google Scholar]
  12. HESS S. M., UDENFRIEND S. A fluorometric procedure for the measurement of tryptamine in tissues. J Pharmacol Exp Ther. 1959 Nov;127:175–177. [PubMed] [Google Scholar]
  13. Hvitefelt J., Santti R. S. Tryptophan pyrrolase in the liver of guinea pig: the absence of hydrocortisone induction. Biochim Biophys Acta. 1972 Feb 28;258(2):358–365. doi: 10.1016/0005-2744(72)90227-6. [DOI] [PubMed] [Google Scholar]
  14. Johnson R. J., Dyer I. A. Effect of orally administered tryptophan on tryptophan pyrrolase activity in ovine and bovine. Life Sci. 1966 Jun;5(12):1121–1124. doi: 10.1016/0024-3205(66)90095-6. [DOI] [PubMed] [Google Scholar]
  15. Knox W. E. The regulation of tryptophan pyrrolase activity by tryptophan. Adv Enzyme Regul. 1966;4:287–297. doi: 10.1016/0065-2571(66)90023-9. [DOI] [PubMed] [Google Scholar]
  16. Yang J. N., Carlson J. R. Effects of high tryptophan doses and two experimental rations on the excretion of urinary tryptophan metabolites in cattle. J Nutr. 1972 Dec;102(12):1655–1665. doi: 10.1093/jn/102.12.1655. [DOI] [PubMed] [Google Scholar]

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