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. 1974 Apr;139(1):237–242. doi: 10.1042/bj1390237

A convenient spectrophotometric assay for the determination of l-ergothioneine in blood

Jan Carlsson 1,*, Marek P J Kierstan 1,†, Keith Brocklehurst 1
PMCID: PMC1166272  PMID: 4463946

Abstract

1. A convenient spectrophotometric assay for the determination of l-ergothioneine in solution including deproteinized blood haemolysate was developed. 2. The method consists of deproteinization by heat precipitation and Cu2+-catalysed oxidation of thiols such as glutathione and of l-ascorbic acid, both in alkaline media, and titration of l-ergothioneine (which is not oxidized under these conditions) by its virtually instantaneous reaction with 2,2′-dipyridyl disulphide at pH1. 3. This method and the results obtained with it for the analysis of human, horse, sheep and pig blood are compared with existing methods of l-ergothioneine analysis and the results obtained thereby.

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Selected References

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