Abstract
Ultrastructural cytochemical study of the nuage in the human adult testis revealed that this structure was a cytoplasmic fine fibrillar electron-dense mass, similar to the chromatoid body of spermatids, in all spermatogonial types and spermatocytes. The nuage was often observed in relation with the nucleus or mitochondria. Cytochemical techniques showed staining affinity of the nuage for both ethanolic phosphotungstic acid and ethylene diamine tetra-acetic acid. The intermitochondrial bars were also stained with the two procedures. The results suggest that the nuage originates from the nucleus and migrates to the cytoplasm through nuclear pores, giving rise to the intermitochondrial bars.
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- ANDRE J. [Contribution to the knowledge of the chondriome. Study of its ultrastructural changes during spermatogenesis]. J Ultrastruct Res. 1962 May;Suppl 3:1–185. [PubMed] [Google Scholar]
- Bernhard W. A new staining procedure for electron microscopical cytology. J Ultrastruct Res. 1969 May;27(3):250–265. doi: 10.1016/s0022-5320(69)80016-x. [DOI] [PubMed] [Google Scholar]
- Comings D. E., Okada T. A. The chromatoid body in mouse spermatogenesis: evidence that it may be formed by the extrusion of nucleolar components. J Ultrastruct Res. 1972 Apr;39(1):15–23. doi: 10.1016/s0022-5320(72)80003-0. [DOI] [PubMed] [Google Scholar]
- Eddy E. M. Fine structural observations on the form and distribution of nuage in germ cells of the rat. Anat Rec. 1974 Apr;178(4):731–757. doi: 10.1002/ar.1091780406. [DOI] [PubMed] [Google Scholar]
- Fawcett D. W., Eddy E. M., Phillips D. M. Observations on the fine structure and relationships of the chromatoid body in mammalian spermatogenesis. Biol Reprod. 1970 Feb;2(1):129–153. doi: 10.1095/biolreprod2.1.129. [DOI] [PubMed] [Google Scholar]
- Gondos B., Zemjanis R. Fine structure of spermatogonia and intercellular bridges in Macaca nemestrina. J Morphol. 1970 Aug;131(4):431–446. doi: 10.1002/jmor.1051310406. [DOI] [PubMed] [Google Scholar]
- Kerr J. B., Dixon K. E. An ultrastructural study of germ plasm in spermatogenesis of Xenopus laevis. J Embryol Exp Morphol. 1974 Dec;32(3):573–592. [PubMed] [Google Scholar]
- Krimer D. B., Esponda P. Presence of polysaccharides and proteins in the chromatoid body of mouse spermatids. Cell Biol Int Rep. 1980 Mar;4(3):265–270. doi: 10.1016/0309-1651(80)90058-2. [DOI] [PubMed] [Google Scholar]
- Nicander L., Plöen L. Fine structure of spermatogonia and primary spermatocytes in rabbits. Z Zellforsch Mikrosk Anat. 1969;99(2):221–234. doi: 10.1007/BF00342223. [DOI] [PubMed] [Google Scholar]
- Parvinen M., Parvinen L. M. Active movements of the chromatoid body. A possible transport mechanism for haploid gene products. J Cell Biol. 1979 Mar;80(3):621–628. doi: 10.1083/jcb.80.3.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Russell L., Frank B. Ultrastructural characterization of nuage in spermatocytes of the rat testis. Anat Rec. 1978 Jan;190(1):79–97. doi: 10.1002/ar.1091900108. [DOI] [PubMed] [Google Scholar]
- Sheridan W. F., Barrnett R. J. Cytochemical studies on chromosome ultrastructure. J Ultrastruct Res. 1969 May;27(3):216–229. doi: 10.1016/s0022-5320(69)80013-4. [DOI] [PubMed] [Google Scholar]
- Stockert J. C., Colman O. D., Giménez-Martín G., Esponda P. Selective staining of rodent acrosomes with phosphotungstic acid. Mikroskopie. 1975 Apr;31(1-2):36–41. [PubMed] [Google Scholar]
- Susi F. R., Clermont Y. Fine structural modifications of the rat chromatoid body during spermiogenesis. Am J Anat. 1970 Oct;129(2):177–191. doi: 10.1002/aja.1001290205. [DOI] [PubMed] [Google Scholar]
- Söderström K. O., Parvinen M. Transport of material between the nucleus, the chromatoid body and the Golgi complex in the early spermatids of the rat. Cell Tissue Res. 1976 May 13;168(3):335–342. doi: 10.1007/BF00215311. [DOI] [PubMed] [Google Scholar]