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. 1986 Feb;5(2):369–373. doi: 10.1002/j.1460-2075.1986.tb04221.x

Transcription of the cdc2 cell cycle control gene of the fission yeast Schizosaccharomyces pombe

Barbara Durkacz 1,1, Antony Carr 1, Paul Nurse 1
PMCID: PMC1166741  PMID: 16453669

Abstract

The cdc2 gene plays a central role in the control of the mitotic cell cycle of the fission yeast Schizosaccharomyces pombe. It is required in G1 at start for commitment to the mitotic cycle and then again in G2 where it determines the timing of mitosis. We have identified the cdc2 gene transcript as a 1.6-kb polyadenylated mRNA. This transcript is generated after four introns have been spliced out; there is no evidence for differential splicing. The level of cdc2 transcript does not change during a shift between cell proliferation and stationary phase or during the mitotic cell cycle. Overproduction of the cdc2 transcript does not alter the normal cell cycle. We conclude that the cell cycle is not controlled by changes in either the cdc2 transcript level or in its processing. A gene adjacent to cdc2 called cdc2L has also been identified. This encodes three transcripts of 1.0–1.3 kb in length, at least two of which are cell cycle regulated. Their levels peak during S-phase and are increased in certain cell cycle mutants. This gene may code for a product which is required for the mitotic cell cycle.

Keywords: cell cycle, Schizosaccharomyces pombe, start

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aves S. J., Durkacz B. W., Carr A., Nurse P. Cloning, sequencing and transcriptional control of the Schizosaccharomyces pombe cdc10 'start' gene. EMBO J. 1985 Feb;4(2):457–463. doi: 10.1002/j.1460-2075.1985.tb03651.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beach D., Durkacz B., Nurse P. Functionally homologous cell cycle control genes in budding and fission yeast. Nature. 1982 Dec 23;300(5894):706–709. doi: 10.1038/300706a0. [DOI] [PubMed] [Google Scholar]
  3. Beach D., Piper M., Nurse P. Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation. Mol Gen Genet. 1982;187(2):326–329. doi: 10.1007/BF00331138. [DOI] [PubMed] [Google Scholar]
  4. Fantes P. A. Isolation of cell size mutants of a fission yeast by a new selective method: characterization of mutants and implications for division control mechanisms. J Bacteriol. 1981 May;146(2):746–754. doi: 10.1128/jb.146.2.746-754.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Groffen J., Heisterkamp N., Reynolds F. H., Jr, Stephenson J. R. Homology between phosphotyrosine acceptor site of human c-abl and viral oncogene products. Nature. 1983 Jul 14;304(5922):167–169. doi: 10.1038/304167a0. [DOI] [PubMed] [Google Scholar]
  6. Hindley J., Phear G. A. Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases. Gene. 1984 Nov;31(1-3):129–134. doi: 10.1016/0378-1119(84)90203-8. [DOI] [PubMed] [Google Scholar]
  7. Huysmans E., Dams E., Vandenberghe A., De Wachter R. The nucleotide sequences of the 5S rRNAs of four mushrooms and their use in studying the phylogenetic position of basidiomycetes among the eukaryotes. Nucleic Acids Res. 1983 May 11;11(9):2871–2880. doi: 10.1093/nar/11.9.2871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lörincz A. T., Reed S. I. Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes. Nature. 1984 Jan 12;307(5947):183–185. doi: 10.1038/307183a0. [DOI] [PubMed] [Google Scholar]
  9. Nasmyth K. A. A control acting over the initiation of DNA replication in the yeast Schizosaccharomyces pombe. J Cell Sci. 1979 Apr;36:155–168. doi: 10.1242/jcs.36.1.155. [DOI] [PubMed] [Google Scholar]
  10. Nurse P., Bissett Y. Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast. Nature. 1981 Aug 6;292(5823):558–560. doi: 10.1038/292558a0. [DOI] [PubMed] [Google Scholar]
  11. Nurse P. Genetic control of cell size at cell division in yeast. Nature. 1975 Aug 14;256(5518):547–551. doi: 10.1038/256547a0. [DOI] [PubMed] [Google Scholar]
  12. Nurse P., Thuriaux P. Controls over the timing of DNA replication during the cell cycle of fission yeast. Exp Cell Res. 1977 Jul;107(2):365–375. doi: 10.1016/0014-4827(77)90358-5. [DOI] [PubMed] [Google Scholar]
  13. Nurse P., Thuriaux P. Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe. Genetics. 1980 Nov;96(3):627–637. doi: 10.1093/genetics/96.3.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shoji S., Parmelee D. C., Wade R. D., Kumar S., Ericsson L. H., Walsh K. A., Neurath H., Long G. L., Demaille J. G., Fischer E. H. Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1981 Feb;78(2):848–851. doi: 10.1073/pnas.78.2.848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thuriaux P., Nurse P., Carter B. Mutants altered in the control co-ordinating cell division with cell growth in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1978 May 3;161(2):215–220. doi: 10.1007/BF00274190. [DOI] [PubMed] [Google Scholar]
  16. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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