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. 1995 Dec;187(Pt 3):603–611.

Expression and upregulation of transferrin receptors and iron uptake in the epiplexus cells of different aged rats injected with lipopolysaccharide and interferon-gamma.

J Lu 1, C Kaur 1, E A Ling 1
PMCID: PMC1167463  PMID: 8586559

Abstract

The expression of transferrin receptors marked by the monoclonal antibody OX-26 and localisation of iron was studied in epiplexus cells in the lateral ventricles of different aged rats subjected to the challenge of the bacterial toxin, lipopolysaccharide, and treatment with interferon-gamma. Transferrin receptor expression in epiplexus cells was extremely low in normal rats, but was vigorously elevated in rats receiving intraperitoneal injections of lipopolysaccharide (LPS); other antigens such as complement type 3 receptors immunostained with OX-42 and macrophage antigen marked by ED-1 were also notably augmented. After 6 successive intraperitoneal injections of interferon-gamma, the expression of transferrin receptors showed only a moderate increase. Using Perls' staining method, some iron-containing epiplexus cells were observed in early normal postnatal rats, but their number was markedly decreased with age. After LPS administration, a moderate increase in the number of iron-containing epiplexus cells was observed in different aged rats. The significance of the upregulation and vigorous expression of transferrin receptors on epiplexus cells is speculative. One possible explanation would be that they facilitate an increase in the acquisition of iron by these cells for storage, oxidative killing and/or immunological activation. The localisation of iron in the choroid plexus in the lateral ventricles suggests that the latter may function as an important storage organ for iron. Present results also suggest the presence of an efficient transport system for the transfer of iron across the interface of the blood and cerebrospinal fluid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benkovic S. A., Connor J. R. Ferritin, transferrin, and iron in selected regions of the adult and aged rat brain. J Comp Neurol. 1993 Dec 1;338(1):97–113. doi: 10.1002/cne.903380108. [DOI] [PubMed] [Google Scholar]
  2. Britigan B. E., Serody J. S., Hayek M. B., Charniga L. M., Cohen M. S. Uptake of lactoferrin by mononuclear phagocytes inhibits their ability to form hydroxyl radical and protects them from membrane autoperoxidation. J Immunol. 1991 Dec 15;147(12):4271–4277. [PubMed] [Google Scholar]
  3. Byrd T. F., Horwitz M. A. Regulation of transferrin receptor expression and ferritin content in human mononuclear phagocytes. Coordinate upregulation by iron transferrin and downregulation by interferon gamma. J Clin Invest. 1993 Mar;91(3):969–976. doi: 10.1172/JCI116318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carpenter S. J., McCarthy L. E., Borison H. L. Electron microscopic study of the epiplexus (Kolmer) cells of the cat choroid plexus. Z Zellforsch Mikrosk Anat. 1970;110(4):471–486. doi: 10.1007/BF00330099. [DOI] [PubMed] [Google Scholar]
  5. Deiss A. Iron metabolism in reticuloendothelial cells. Semin Hematol. 1983 Apr;20(2):81–90. [PubMed] [Google Scholar]
  6. Dijkstra C. D., Döpp E. A., Joling P., Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 1985 Mar;54(3):589–599. [PMC free article] [PubMed] [Google Scholar]
  7. Dwork A. J., Schon E. A., Herbert J. Nonidentical distribution of transferrin and ferric iron in human brain. Neuroscience. 1988 Oct;27(1):333–345. doi: 10.1016/0306-4522(88)90242-4. [DOI] [PubMed] [Google Scholar]
  8. Espinosa de los Monteros A., Foucaud B. Effect of iron and transferrin on pure oligodendrocytes in culture; characterization of a high-affinity transferrin receptor at different ages. Brain Res. 1987 Sep;432(1):123–130. doi: 10.1016/0165-3806(87)90014-9. [DOI] [PubMed] [Google Scholar]
  9. Giometto B., Bozza F., Argentiero V., Gallo P., Pagni S., Piccinno M. G., Tavolato B. Transferrin receptors in rat central nervous system. An immunocytochemical study. J Neurol Sci. 1990 Aug;98(1):81–90. doi: 10.1016/0022-510x(90)90183-n. [DOI] [PubMed] [Google Scholar]
  10. Hamilton T. A. Regulation of transferrin receptor expression in concanavalin A stimulated and Gross virus transformed rat lymphoblasts. J Cell Physiol. 1982 Oct;113(1):40–46. doi: 10.1002/jcp.1041130109. [DOI] [PubMed] [Google Scholar]
  11. Hamilton T. A., Weiel J. E., Adams D. O. Expression of the transferrin receptor in murine peritoneal macrophages is modulated in the different stages of activation. J Immunol. 1984 May;132(5):2285–2290. [PubMed] [Google Scholar]
  12. Hill J. M., Switzer R. C., 3rd The regional distribution and cellular localization of iron in the rat brain. Neuroscience. 1984 Mar;11(3):595–603. doi: 10.1016/0306-4522(84)90046-0. [DOI] [PubMed] [Google Scholar]
  13. Jefferies W. A., Brandon M. R., Hunt S. V., Williams A. F., Gatter K. C., Mason D. Y. Transferrin receptor on endothelium of brain capillaries. Nature. 1984 Nov 8;312(5990):162–163. doi: 10.1038/312162a0. [DOI] [PubMed] [Google Scholar]
  14. Kaur C., Ling E. A., Gopalakrishnakone P., Wong W. C. Response of intraventricular macrophages to crotoxin-coated microcarrier beads injected into the lateral ventricle of postnatal rats. J Anat. 1990 Feb;168:63–72. [PMC free article] [PubMed] [Google Scholar]
  15. Laskey J., Webb I., Schulman H. M., Ponka P. Evidence that transferrin supports cell proliferation by supplying iron for DNA synthesis. Exp Cell Res. 1988 May;176(1):87–95. doi: 10.1016/0014-4827(88)90123-1. [DOI] [PubMed] [Google Scholar]
  16. Lazarus A. H., Baines M. G. Studies on the mechanism of specificity of human natural killer cells for tumor cells: correlation between target cell transferrin receptor expression and competitive activity. Cell Immunol. 1985 Dec;96(2):255–266. doi: 10.1016/0008-8749(85)90358-2. [DOI] [PubMed] [Google Scholar]
  17. Lin H. H., Connor J. R. The development of the transferrin-transferrin receptor system in relation to astrocytes, MBP and galactocerebroside in normal and myelin-deficient rat optic nerves. Brain Res Dev Brain Res. 1989 Oct 1;49(2):281–293. doi: 10.1016/0165-3806(89)90029-1. [DOI] [PubMed] [Google Scholar]
  18. Ling E. A. Scanning electron microscopic study of epiplexus cells in the lateral ventricles of the monkey (Macaca fascicularis). J Anat. 1983 Dec;137(Pt 4):645–652. [PMC free article] [PubMed] [Google Scholar]
  19. Ling E. A. Ultrastruct and origin of epiplexus cells in the telencephalic choroid plexus of postnatal rats studied by intravenous injection of carbon particles. J Anat. 1979 Oct;129(Pt 3):479–492. [PMC free article] [PubMed] [Google Scholar]
  20. Ling E. A. Ultrastructure and mode of formation of epiplexus cells in the choroid plexus in the lateral ventricles of the monkey (Macaca fascicularis). J Anat. 1981 Dec;133(Pt 4):555–569. [PMC free article] [PubMed] [Google Scholar]
  21. Lu J., Kaur C., Ling E. A. Immunophenotypic features of epiplexus cells and their response to interferon gamma injected intraperitoneally in postnatal rats. J Anat. 1994 Aug;185(Pt 1):75–84. [PMC free article] [PubMed] [Google Scholar]
  22. Lu J., Kaur C., Ling E. A. Intraventricular macrophages in the lateral ventricles with special reference to epiplexus cells: a quantitative analysis and their uptake of fluorescent tracer injected intraperitoneally in rats of different ages. J Anat. 1993 Oct;183(Pt 2):405–414. [PMC free article] [PubMed] [Google Scholar]
  23. Lu J., Kaur C., Ling E. A. Up-regulation of surface antigens on epiplexus cells in postnatal rats following intraperitoneal injections of lipopolysaccharide. Neuroscience. 1994 Dec;63(4):1169–1178. doi: 10.1016/0306-4522(94)90581-9. [DOI] [PubMed] [Google Scholar]
  24. Lu J., Kaur C., Ling E. A. Uptake of tracer by the epiplexus cells via the choroid plexus epithelium following an intravenous or intraperitoneal injection of horseradish peroxidase in rats. J Anat. 1993 Dec;183(Pt 3):609–617. [PMC free article] [PubMed] [Google Scholar]
  25. Maxwell W. L., Hardy I. G., Watt C., McGadey J., Graham D. I., Adams J. H., Gennarelli T. A. Changes in the choroid plexus, responses by intrinsic epiplexus cells and recruitment from monocytes after experimental head acceleration injury in the non-human primate. Acta Neuropathol. 1992;84(1):78–84. doi: 10.1007/BF00427218. [DOI] [PubMed] [Google Scholar]
  26. Maxwell W. L., McGadey J. Response of intraventricular macrophages after a penetrant cerebral lesion. J Anat. 1988 Oct;160:145–155. [PMC free article] [PubMed] [Google Scholar]
  27. McLean I. W., Nakane P. K. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem. 1974 Dec;22(12):1077–1083. doi: 10.1177/22.12.1077. [DOI] [PubMed] [Google Scholar]
  28. Neckers L. M., Yenokida G., James S. P. The role of the transferrin receptor in human B lymphocyte activation. J Immunol. 1984 Nov;133(5):2437–2441. [PubMed] [Google Scholar]
  29. Oh T. H., Markelonis G. J., Royal G. M., Bregman B. S. Immunocytochemical distribution of transferrin and its receptor in the developing chicken nervous system. Brain Res. 1986 Dec;395(2):207–220. doi: 10.1016/s0006-8993(86)80199-8. [DOI] [PubMed] [Google Scholar]
  30. Pardridge W. M., Eisenberg J., Yang J. Human blood-brain barrier transferrin receptor. Metabolism. 1987 Sep;36(9):892–895. doi: 10.1016/0026-0495(87)90099-0. [DOI] [PubMed] [Google Scholar]
  31. Raivich G., Graeber M. B., Gehrmann J., Kreutzberg G. W. Transferrin Receptor Expression and Iron Uptake in the Injured and Regenerating Rat Sciatic Nerve. Eur J Neurosci. 1991;3(10):919–927. doi: 10.1111/j.1460-9568.1991.tb00027.x. [DOI] [PubMed] [Google Scholar]
  32. Roberts R. L., Fine R. E., Sandra A. Receptor-mediated endocytosis of transferrin at the blood-brain barrier. J Cell Sci. 1993 Feb;104(Pt 2):521–532. doi: 10.1242/jcs.104.2.521. [DOI] [PubMed] [Google Scholar]
  33. Roskams A. J., Connor J. R. Transferrin receptor expression in myelin deficient (md) rats. J Neurosci Res. 1992 Mar;31(3):421–427. doi: 10.1002/jnr.490310304. [DOI] [PubMed] [Google Scholar]
  34. Sturrock R. R. A developmental study of epiplexus cells and supraependymal cells and their possible relationship to microglia. Neuropathol Appl Neurobiol. 1978 Sep-Oct;4(5):307–322. doi: 10.1111/j.1365-2990.1978.tb01345.x. [DOI] [PubMed] [Google Scholar]
  35. Taetle R., Honeysett J. M. Gamma-interferon modulates human monocyte/macrophage transferrin receptor expression. Blood. 1988 Jun;71(6):1590–1595. [PubMed] [Google Scholar]
  36. Taylor E. M., Morgan E. H. Developmental changes in transferrin and iron uptake by the brain in the rat. Brain Res Dev Brain Res. 1990 Aug 1;55(1):35–42. doi: 10.1016/0165-3806(90)90103-6. [DOI] [PubMed] [Google Scholar]

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