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. 1996 Apr;188(Pt 2):383–393.

Morphometric study on the uterine horn and thyroid gland in hypothyroid, and thyroxine treated hypothyroid rats.

I Inuwa 1, M A Williams 1
PMCID: PMC1167575  PMID: 8621338

Abstract

A wide range of reproductive disorders such as irregular menstruation and frank infertility is found in women with hypothyroidism. Most research done on these patients has focused on steroid and gonadotropin hormone profiles, whilst there has been little work on uterine morphology. Studies on hypothyroid animals have also demonstrated increases in fetal wastage, but there have been few studies of uterine structure in the hypothyroid rat. The present study has used hypothyroid Wistar rats as a model for investigating the effects of hypothyroidism on uterine structure. Three groups of Wistar rats were studied. One was made hypothyroid with methimazole (MMI), the 2nd was also made hypothyroid with methimazole but in addition the rats were simultaneously given daily thyroxine intraperitoneally (MMI + T4), and the 3rd was an untreated euthyroid group (control). Daily vaginal smears were obtained from rats in all 3 groups. All rats were aged 6 wk at the start of treatment and were killed after a further 6 wk. Uterine horns were removed and studied. Systematic random transverse sections were obtained from the proximal, middle, and distal regions of the horn and subjected to morphometric analysis. Difference between regions was assessed using 2-way analysis of variance. Absolute volume of endometrium in the uteri of hypothyroid rats was reduced by 45.1% (P < 0.05), whilst that of the muscle layer was decreased by 33.6% (P < 0.05). The cross-sectional area and absolute volume of the uterine horns were also reduced in hypothyroid animals (P < 0.05). In hypothyroid rats given thyroxine (MMI + T4) all variables increased significantly above those of hypothyroid rats. These changes suggest that hypothyroidism has an effect on uterine structure, which demonstrably improves under exogenous thyroxine administration. The observed structural changes might well play a significant role in the reproductive difficulties observed during hypothyroidism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akande E. O. Plasma concentration of gonadotrophins, oestrogen and progesterone in hypothyroid women. Br J Obstet Gynaecol. 1975 Jul;82(7):552–556. doi: 10.1111/j.1471-0528.1975.tb00685.x. [DOI] [PubMed] [Google Scholar]
  2. Balen A. H., Kurtz A. B. Successful outcome of pregnancy with severe hypothyroidism. Case report and literature review. Br J Obstet Gynaecol. 1990 Jun;97(6):536–539. doi: 10.1111/j.1471-0528.1990.tb02526.x. [DOI] [PubMed] [Google Scholar]
  3. Boylan P., Drury M. I. Pregnancy in untreated hypothyroidism. Ir J Med Sci. 1979 Jan;148(1):10–11. doi: 10.1007/BF02938040. [DOI] [PubMed] [Google Scholar]
  4. DeCherney A., Polan M. L. Evaluation and management of habitual abortion. Br J Hosp Med. 1984 Apr;31(4):261-2, 266-8. [PubMed] [Google Scholar]
  5. Distiller L. A., Sagel J., Morley J. E., Oxenham E. Assessment of pituitary gonadotropin reserve using luteinizing hormone-releasing hormone (LRH) in states of altered thyroid function. J Clin Endocrinol Metab. 1975 Mar;40(3):512–515. doi: 10.1210/jcem-40-3-512. [DOI] [PubMed] [Google Scholar]
  6. EVANS E. S., ROSENBERG L. L., SIMPSON M. E. Relative sensitivity of different biological responses to thyroxine. Endocrinology. 1960 Mar;66:433–440. doi: 10.1210/endo-66-3-433. [DOI] [PubMed] [Google Scholar]
  7. Evans R. W., Farwell A. P., Braverman L. E. Nuclear thyroid hormone receptor in the rat uterus. Endocrinology. 1983 Oct;113(4):1459–1463. doi: 10.1210/endo-113-4-1459. [DOI] [PubMed] [Google Scholar]
  8. Fish L. H., Mariash C. N. Hyperprolactinemia, infertility, and hypothyroidism. A case report and literature review. Arch Intern Med. 1988 Mar;148(3):709–711. [PubMed] [Google Scholar]
  9. GOLDSMITH R. E., STURGIS S. H., LERMAN J., STANBURY J. B. The menstrual pattern in thyroid disease. J Clin Endocrinol Metab. 1952 Jul;12(7):846–855. doi: 10.1210/jcem-12-7-846. [DOI] [PubMed] [Google Scholar]
  10. Gundersen H. J., Jensen E. B. The efficiency of systematic sampling in stereology and its prediction. J Microsc. 1987 Sep;147(Pt 3):229–263. doi: 10.1111/j.1365-2818.1987.tb02837.x. [DOI] [PubMed] [Google Scholar]
  11. Hardy M. P., Kirby J. D., Hess R. A., Cooke P. S. Leydig cells increase their numbers but decline in steroidogenic function in the adult rat after neonatal hypothyroidism. Endocrinology. 1993 Jun;132(6):2417–2420. doi: 10.1210/endo.132.6.8504746. [DOI] [PubMed] [Google Scholar]
  12. Hemady Z. S., Siler-Khodr T. M., Najjar S. Precocious puberty in juvenile hypothyroidism. J Pediatr. 1978 Jan;92(1):55–59. doi: 10.1016/s0022-3476(78)80070-5. [DOI] [PubMed] [Google Scholar]
  13. Heyburn P. J., Gibby O. M., Hourihan M., Hall R., Scanlon M. F. Primary hypothyroidism presenting as amenorrhoea and galactorrhoea with hyperprolactinaemia and pituitary enlargement. Br Med J (Clin Res Ed) 1986 Jun 21;292(6536):1660–1661. doi: 10.1136/bmj.292.6536.1660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Imamura Y. [Uterine response to estrogen in the rat of hypothyroidism]. Nihon Naibunpi Gakkai Zasshi. 1982 Jul 20;58(7):867–875. doi: 10.1507/endocrine1927.58.7_867. [DOI] [PubMed] [Google Scholar]
  15. KROHN P. L., WHITE H. C. The effect of hypothyroidism on reproduction in the female albino rat. J Endocrinol. 1950 Jul;6(4):375–385. [PubMed] [Google Scholar]
  16. Kennedy A. L., Montgomery D. A. Hypothyroidism in pregnancy. Br J Obstet Gynaecol. 1978 Mar;85(3):225–230. doi: 10.1111/j.1471-0528.1978.tb10487.x. [DOI] [PubMed] [Google Scholar]
  17. Kirby J. D., Jetton A. E., Cooke P. S., Hess R. A., Bunick D., Ackland J. F., Turek F. W., Schwartz N. B. Developmental hormonal profiles accompanying the neonatal hypothyroidism-induced increase in adult testicular size and sperm production in the rat. Endocrinology. 1992 Aug;131(2):559–565. doi: 10.1210/endo.131.2.1639007. [DOI] [PubMed] [Google Scholar]
  18. Meisami E., Sendera T. J., Clay L. B. Paradoxical hypertrophy and plasticity of the testis in rats recovering from early thyroid deficiency: a growth study including effects of age and duration of hypothyroidism. J Endocrinol. 1992 Dec;135(3):495–505. doi: 10.1677/joe.0.1350495. [DOI] [PubMed] [Google Scholar]
  19. Montoro M., Collea J. V., Frasier S. D., Mestman J. H. Successful outcome of pregnancy in women with hypothyroidism. Ann Intern Med. 1981 Jan;94(1):31–34. doi: 10.7326/0003-4819-94-1-31. [DOI] [PubMed] [Google Scholar]
  20. Mukku V. R., Kirkland J. L., Hardy M., Stancel G. M. Evidence for thyroid hormone receptors in uterine nuclei. Metabolism. 1983 Feb;32(2):142–145. doi: 10.1016/0026-0495(83)90219-6. [DOI] [PubMed] [Google Scholar]
  21. Ortega E., Rodriguez E., Ruiz E., Osorio C. Activity of the hypothalamo-pituitary ovarian axis in hypothyroid rats with or without triiodothyronine replacement. Life Sci. 1990;46(6):391–395. doi: 10.1016/0024-3205(90)90081-2. [DOI] [PubMed] [Google Scholar]
  22. Silva J. E., Leonard J. L. Regulation of rat cerebrocortical and adenohypophyseal type II 5'-deiodinase by thyroxine, triiodothyronine, and reverse triiodothyronine. Endocrinology. 1985 Apr;116(4):1627–1635. doi: 10.1210/endo-116-4-1627. [DOI] [PubMed] [Google Scholar]
  23. Valle L. B., Oliveira-Filho R. M., Romaldini J. H., Lara P. F. Pituitary-testicular axis abnormalities in immature male hypothyroid rats. J Steroid Biochem. 1985 Sep;23(3):253–257. doi: 10.1016/0022-4731(85)90402-9. [DOI] [PubMed] [Google Scholar]
  24. Vriend J., Bertalanffy F. D., Ralcewicz T. A. The effects of melatonin and hypothyroidism on estradiol and gonadotropin levels in female Syrian hamsters. Biol Reprod. 1987 Apr;36(3):719–728. doi: 10.1095/biolreprod36.3.719. [DOI] [PubMed] [Google Scholar]

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