Skip to main content
PMC home page
Journal of Anatomy logoLink to Journal of Anatomy
. 1981 Sep;133(Pt 2):281–300.

Fibroblast cell population kinetics in the mouse molar periodontal ligament and tooth eruption.

K A Perera, C H Tonge
PMCID: PMC1167671  PMID: 7333954

Abstract

Fibroblast cell population kinetics in the developing molar periodontal ligament was investigated in 10, 12, 16 and 20 days old mice by autoradiography after the administration of [3H]thymidine. Labelled mitoses, in number per unit area, were counted over the apical zones of the sections and percentage labelled mitoses (PLM) curves were drawn. Median values for some phases durations were read off at the 0.5 level of mitotic labelling. In determining other kinetic parameters the periodontal fibroblast population was considered separately as (1) a steady state system, (2) an exponentially growing system. An attempt was made to estimate mean values for these parameters using the Gilbert Computer programme. The programme generated the original data points and the fitted curve in graphical and numerical form together with the mean values and standard errors. The fact that the Gilbert programme assumes a stationary population in its theoretical PLM curve analysis was used to establish the kinetic type of the periodontal fibroblast population. The present study has demonstrated that in the growing periodontal ligament where cell specialisation and migration occurs steady state system is the kinetic model applicable. Failure of the computed PLM curves to fit the data adequately confirmed the fibroblast migration (apico-occlusal migration) from the apical zone to other zones. A definite cytokinetic basis for the periodontal ligament fibroblast proliferation and migration was established. Accordingly, fibroblast proliferation and migration takes place by a reduction in the DNA synthesis time (T8), cell generation tie (Tc) and possibly by a similar reduction in other parameters. Maximum reduction in these parameters is associated with the peak proliferative and migratory activity in the 12 days old group which is also the time that tooth eruption takes place in the mouse. Thus fibroblast proliferation and migration are major causative factors responsible for tooth eruption. Based on these results, a mechanism for tooth eruption is proposed.

Full text

PDF
283

Images in this article

295Fig. 7
on p.295
296Fig. 8
on p.296

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett J. C. A mathematical model of the mitotic cycle and its application to the interpretation of percentage labeled mitoses data. J Natl Cancer Inst. 1966 Oct;37(4):443–450. [PubMed] [Google Scholar]
  2. Barrett J. C. Optimized parameters for the mitotic cycle. Cell Tissue Kinet. 1970 Oct;3(4):349–353. doi: 10.1111/j.1365-2184.1970.tb00342.x. [DOI] [PubMed] [Google Scholar]
  3. Baserga R., Wiebel F. The cell cycle of mammalian cells. Int Rev Exp Pathol. 1969;7:1–30. [PubMed] [Google Scholar]
  4. Beertsen W., Everts V., van den Hooff A. Fine structure of fibroblasts in the periodontal ligament of the rat incisor and their possible role in tooth eruption. Arch Oral Biol. 1974 Dec;19(12):1087–1098. doi: 10.1016/0003-9969(74)90235-0. [DOI] [PubMed] [Google Scholar]
  5. Bronk B. V. On radioactive labeling of proliferating cells: the graph of labeled mitosis. J Theor Biol. 1969 Mar;22(3):468–492. doi: 10.1016/0022-5193(69)90016-2. [DOI] [PubMed] [Google Scholar]
  6. Cleaver J. E. The relationship between the duration of the S phase and the fraction of cells which incorporate 3-H-thymidine during exponential growth. Exp Cell Res. 1965 Sep;39(2):697–700. doi: 10.1016/0014-4827(65)90074-1. [DOI] [PubMed] [Google Scholar]
  7. Gilbert C. W. The labelled mitoses curve and the estimation of the parameters of the cell cycle. Cell Tissue Kinet. 1972 Jan;5(1):53–63. doi: 10.1111/j.1365-2184.1972.tb00355.x. [DOI] [PubMed] [Google Scholar]
  8. Kameyama Y. The pattern of alveolar bone activity during development and eruption of the molar in the rat. J Periodontal Res. 1973;8(3):179–191. doi: 10.1111/j.1600-0765.1973.tb01756.x. [DOI] [PubMed] [Google Scholar]
  9. LEBLOND C. P. CLASSIFICATION OF CELL POPULATIONS ON THE BASIS OF THEIR PROLIFERATIVE BEHAVIOR. Natl Cancer Inst Monogr. 1964 May;14:119–150. [PubMed] [Google Scholar]
  10. Melcher A. H., Correia M. A. Remodelling of periodontal ligament in erupting molars of mature rats. J Periodontal Res. 1971;6(2):118–125. doi: 10.1111/j.1600-0765.1971.tb00598.x. [DOI] [PubMed] [Google Scholar]
  11. NESS A. R. MOVEMENT AND FORCES IN TOOTH ERUPTION. Adv Oral Biol. 1964;1:33–75. doi: 10.1016/b978-1-4832-3117-4.50008-5. [DOI] [PubMed] [Google Scholar]
  12. Perera K. A., Tonge C. H. Fibroblast cell proliferation in the mouse molar periodontal ligament. J Anat. 1981 Aug;133(Pt 1):77–90. [PMC free article] [PubMed] [Google Scholar]
  13. Roberts W. E., Chase D. C., Jee S. S. Counts of labelled mitoses in the orthodontically-stimulated periodontal ligament in the rat. Arch Oral Biol. 1974 Aug;19(8):665–670. doi: 10.1016/0003-9969(74)90135-6. [DOI] [PubMed] [Google Scholar]
  14. Roberts W. E., Jee W. S. Cell kinetics of orthodontically-stimulated and non-stimulated periodontal ligament in the rat. Arch Oral Biol. 1974 Jan;19(1):17–21. doi: 10.1016/0003-9969(74)90219-2. [DOI] [PubMed] [Google Scholar]
  15. SMITH C. L., DENDY P. P. Relation between mitotic index, duration of mitosis, generation time and fraction of dividing cells in a cell population. Nature. 1962 Feb 10;193:555–556. doi: 10.1038/193555a0. [DOI] [PubMed] [Google Scholar]
  16. Simnett J. D., Heppleston A. G. Cell renewal in the mouse lung. The influence of sex, strain, and age. Lab Invest. 1966 Nov;15(11):1793–1801. [PubMed] [Google Scholar]
  17. Steel G. G., Hanes S. The technique of labelled mitoses: analysis by automatic curve-fitting. Cell Tissue Kinet. 1971 Jan;4(1):93–105. doi: 10.1111/j.1365-2184.1971.tb01520.x. [DOI] [PubMed] [Google Scholar]
  18. Takahashi M. Theoretical basis for cell cycle analysis: II. Further studies on labelled mitosis wave method. J Theor Biol. 1968 Feb;18(2):195–209. doi: 10.1016/0022-5193(68)90161-6. [DOI] [PubMed] [Google Scholar]
  19. Ten Cate A. R., Deporter D. A. The role of the fibroblast in collagen turnover in the functioning periodontal ligament of the mouse. Arch Oral Biol. 1974 Apr;19(4):339–340. doi: 10.1016/0003-9969(74)90199-x. [DOI] [PubMed] [Google Scholar]
  20. Trucco E., Brockwell P. J. Percentage labeled mitoses curves in exponentially growing cell populations. J Theor Biol. 1968 Sep;20(3):321–337. doi: 10.1016/0022-5193(68)90132-x. [DOI] [PubMed] [Google Scholar]
  21. Zajicek G. Fibroblast cell kinetics in the periodontal ligament of the mouse. Cell Tissue Kinet. 1974 Sep;7(5):479–492. doi: 10.1111/j.1365-2184.1974.tb00431.x. [DOI] [PubMed] [Google Scholar]
  22. ten Cate A. R. Cell division and periodontal ligament formation in the mouse. Arch Oral Biol. 1972 Dec;17(12):1781–1784. doi: 10.1016/0003-9969(72)90242-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Anatomy are provided here courtesy of Anatomical Society of Great Britain and Ireland

RESOURCES