Abstract
Uptake and translocation of particulates across the mucosal barrier of the gastrointestinal (GI) tract is now generally recognised but the effect of pathophysiologically induced changes on this process is less well established. This study evaluated the effect of diabetes mellitus on GI absorption of particles, comparing particle localisation and particle loading in different microanatomical sites of the primary organ (small intestine) and possible particle translocation pathways to selected secondary organs (mesenteric lymph nodes, liver, spleen) in normal and streptozotocin-induced diabetic animals. Fluorescent polystyrene latex particles (approximately 2 microns diameter) were fed orally to young adult Sprague-Dawley rats and quantitative bulk tissue and morphological techniques used to chart particle transit across the small intestine to secondary organs 0.5 h postadministration. In the normal animal, epifluorescence and confocal laser scanning microscopy provided confirmatory evidence for particle absorption within the primary organ and transport to other sites in the body. By contrast, in the diabetic animal, particle translocation and peripheral distribution were reduced with approximately 30% decrease in particle loading in the epithelial/nonepithelial tissue compartments. This could be a consequence of gastric retention and altered intestinal motility and permeability which are known to be associated with diabetes.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bergström S., Norrby K. Hyperplasia of the mesenterial windows precedes that of the small gut in the streptozotocin-diabetic rat. APMIS. 1988 May;96(5):407–414. doi: 10.1111/j.1699-0463.1988.tb05323.x. [DOI] [PubMed] [Google Scholar]
- Deitch E. A. Bacterial translocation: the influence of dietary variables. Gut. 1994 Jan;35(1 Suppl):S23–S27. doi: 10.1136/gut.35.1_suppl.s23. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fedorak R. N. Adaptation of small intestinal membrane transport processes during diabetes mellitus in rats. Can J Physiol Pharmacol. 1990 May;68(5):630–635. doi: 10.1139/y90-092. [DOI] [PubMed] [Google Scholar]
- Hodges G. M., Carr E. A., Hazzard R. A., Carr K. E. Uptake and translocation of microparticles in small intestine. Morphology and quantification of particle distribution. Dig Dis Sci. 1995 May;40(5):967–975. doi: 10.1007/BF02064184. [DOI] [PubMed] [Google Scholar]
- Jani P., Halbert G. W., Langridge J., Florence A. T. Nanoparticle uptake by the rat gastrointestinal mucosa: quantitation and particle size dependency. J Pharm Pharmacol. 1990 Dec;42(12):821–826. doi: 10.1111/j.2042-7158.1990.tb07033.x. [DOI] [PubMed] [Google Scholar]
- Jani P., Halbert G. W., Langridge J., Florence A. T. The uptake and translocation of latex nanospheres and microspheres after oral administration to rats. J Pharm Pharmacol. 1989 Dec;41(12):809–812. doi: 10.1111/j.2042-7158.1989.tb06377.x. [DOI] [PubMed] [Google Scholar]
- Mantle M., Thakore E., Atkins E., Mathison R., Davison J. S. Effects of streptozotocin-diabetes on rat intestinal mucin and goblet cells. Gastroenterology. 1989 Jul;97(1):68–75. doi: 10.1016/0016-5085(89)91417-0. [DOI] [PubMed] [Google Scholar]
- Mathison R., Davison J. S. Modified smooth muscle responses of jejunum in streptozotocin-diabetic rats. J Pharmacol Exp Ther. 1988 Mar;244(3):1045–1050. [PubMed] [Google Scholar]
- Miller D. L., Hanson W., Schedl H. P., Osborne J. W. Proliferation rate and transit time of mucosal cells in small intestine of the diabetic rat. Gastroenterology. 1977 Dec;73(6):1326–1332. [PubMed] [Google Scholar]
- Van Leeuwen P. A., Boermeester M. A., Houdijk A. P., Ferwerda C. C., Cuesta M. A., Meyer S., Wesdorp R. I. Clinical significance of translocation. Gut. 1994 Jan;35(1 Suppl):S28–S34. doi: 10.1136/gut.35.1_suppl.s28. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Volkheimer G. Persorption of particles: physiology and pharmacology. Adv Pharmacol Chemother. 1977;14:163–187. doi: 10.1016/s1054-3589(08)60188-x. [DOI] [PubMed] [Google Scholar]