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. 1981 Aug;133(Pt 1):77–90.

Fibroblast cell proliferation in the mouse molar periodontal ligament.

K A Perera, C H Tonge
PMCID: PMC1167726  PMID: 7319901

Abstract

Cytogenesis of the developing molar periodontal ligament was studied by determining labelling indices in 4 groups of young mice. Autoradiographs were made using paraffin sections of demineralised specimens from 10 days, 12 days, 16 days and 20 days old mice allowed to survive a varying period (1--96 hours) after the administration of [3H]thymidine. Labelled fibroblasts in number per unit area were counted over three different zones (apical, middle and cervical) of the sections and labelling index distribution curves were drawn. The present study has demonstrated the existence of a progenitor area with proliferating fibroblasts in the apical zone and a migration of these cells into other zones (middle and cervical). However, it has also shown that a measurable proliferative rate is present at all levels of the molar periodontal ligament in all ages of animals investigated. Such an observation if compatible with fibroblasts migrating from the apical zone in an occlusal direction and maintaining a decreasing degree of proliferative activity as they progress. Consequently, a tentative apico-occlusal migratory model for the periodontal ligament fibroblast proliferation and migration is proposed. Accordingly, fibroblast proliferation takes place in the apical progenitor zone, and cells migrate from the apical zone to middle and cervical zones, but maintaining a decreasing degree of proliferative activity as they migrate. Peak proliferative and migratory activity is seen in the 12 days group which is also the time that tooth eruption takes place in the mouse. Thus, a close relationship exists between fibroblast cell proliferation, migration and tooth eruption.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abercrombie M., Heaysman J. E., Pegrum S. M. The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella. Exp Cell Res. 1971 Aug;67(2):359–367. doi: 10.1016/0014-4827(71)90420-4. [DOI] [PubMed] [Google Scholar]
  2. Beertsen W., Everts V., van den Hooff A. Fine structure of fibroblasts in the periodontal ligament of the rat incisor and their possible role in tooth eruption. Arch Oral Biol. 1974 Dec;19(12):1087–1098. doi: 10.1016/0003-9969(74)90235-0. [DOI] [PubMed] [Google Scholar]
  3. Beertsen W. Tissue dynamics in the periodontal ligament of the mandibular incisor of the mouse: a preliminary report. Arch Oral Biol. 1973 Jan;18(1):61–66. doi: 10.1016/0003-9969(73)90020-4. [DOI] [PubMed] [Google Scholar]
  4. Chiba M. Cellular proliferation in the tooth germ of the rat incisor. Arch Oral Biol. 1965 Jul-Aug;10(4):707–718. doi: 10.1016/0003-9969(65)90015-4. [DOI] [PubMed] [Google Scholar]
  5. Melcher A. H. Remodelling of the periodontal ligament during eruption of the rat incisor. Arch Oral Biol. 1967 Dec;12(12):1649–1651. doi: 10.1016/0003-9969(67)90199-9. [DOI] [PubMed] [Google Scholar]
  6. NESS A. R. MOVEMENT AND FORCES IN TOOTH ERUPTION. Adv Oral Biol. 1964;1:33–75. doi: 10.1016/b978-1-4832-3117-4.50008-5. [DOI] [PubMed] [Google Scholar]
  7. Ross R. The fibroblast and wound repair. Biol Rev Camb Philos Soc. 1968 Feb;43(1):51–96. doi: 10.1111/j.1469-185x.1968.tb01109.x. [DOI] [PubMed] [Google Scholar]
  8. Ten Cate A. R., Mills C., Solomon G. The development of the periodontium. A transplantation and autoradiographic study. Anat Rec. 1971 Jul;170(3):365–379. doi: 10.1002/ar.1091700312. [DOI] [PubMed] [Google Scholar]
  9. Ten Cate A. R., Mills C. The development of the periodontium: the origin of alveolar bone. Anat Rec. 1972 May;173(1):69–77. doi: 10.1002/ar.1091730106. [DOI] [PubMed] [Google Scholar]
  10. Van Winkle W., Jr The fibroblast in wound healing. Surg Gynecol Obstet. 1967 Feb;124(2):369–386. [PubMed] [Google Scholar]
  11. Van Winkle W., Jr Wound contraction. Surg Gynecol Obstet. 1967 Jul;125(1):131–142. [PubMed] [Google Scholar]
  12. Vasiliev J. M., Gelfand I. M., Domnina L. V., Ivanova O. Y., Komm S. G., Olshevskaja L. V. Effect of colcemid on the locomotory behaviour of fibroblasts. J Embryol Exp Morphol. 1970 Nov;24(3):625–640. [PubMed] [Google Scholar]
  13. Zajicek G. Fibroblast cell kinetics in the periodontal ligament of the mouse. Cell Tissue Kinet. 1974 Sep;7(5):479–492. doi: 10.1111/j.1365-2184.1974.tb00431.x. [DOI] [PubMed] [Google Scholar]
  14. ten Cate A. R. Cell division and periodontal ligament formation in the mouse. Arch Oral Biol. 1972 Dec;17(12):1781–1784. doi: 10.1016/0003-9969(72)90242-7. [DOI] [PubMed] [Google Scholar]

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