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. 1982 Mar;134(Pt 2):399–405.

The behaviour of cow blastocyst in vitro: cinematographic and morphometric analysis.

A Massip, J Mulnard, P Vanderzwalmen, C Hanzen, F Ectors
PMCID: PMC1167926  PMID: 7076563

Abstract

The behaviour of the cow blastocyst in vitro was studied by time-lapse cinematography and analysed by morphometry. Three types of behaviour were observed: continuous expansion followed by hatching; discontinuous expansion interrupted by few contractions and followed by hatching; discontinuous expansion interrupted by several rapid contractions without hatching. This demonstrated that the pulsatile activity of the blastocyst is not a necessary condition of hatching but also that only a moderate pulsatile activity is compatible with normal hatching. The time of hatching in vitro corresponded approximately with the time of zona loss in vivo (9-10 days). Rupture of the zona occurred at any point of the trophoblast layer. Hatching by herniation through a reduced opening of the zona was occasionally observed. The behavior of the embryos from a particular animal was very similar but differences were noted between embryos from different animals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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