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. 1974 May;140(2):135–142. doi: 10.1042/bj1400135

The effect of nickel on secretory systems. Studies on the release of amylase, insulin and growth hormone

Robert L Dormer 1, Alan L Kerbey 1, Margaret McPherson 1, Susan Manley 1, Stephen J H Ashcroft 1, J George Schofield 1, Philip J Randle 1
PMCID: PMC1167985  PMID: 4375956

Abstract

The effects of Ni2+ on the release of amylase from rat parotids, insulin from mouse pancreatic islets and growth hormone from bovine pituitary slices were investigated. In all these secretory systems, Ni2+ was shown to inhibit release evoked by a variety of stimuli both physiological and pharmacological. Measurements of rates of substrate oxidation and tissue concentrations of ATP and 3′:5′-cyclic AMP suggest that this inhibitory action of Ni2+ does not arise through an effect on energy metabolism or cyclic AMP metabolism. It is concluded that although some effects of Ni2+ may involve antagonism between Ni2+ and Ca2+ in stimulus–secretion coupling, others appear to be independent of Ca2+. It is suggested that Ni2+ may block exocytosis by interfering with either secretory-granule migration or membrane fusion and microvillus formation. The possible mode of action of Ni2+ and its potential use as a tool in the study of exocytosis are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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