Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1974 Aug;141(2):407–411. doi: 10.1042/bj1410407

A new assay procedure for monoglyceride acyltransferase

Verena J Short 1, David N Brindley 1, Raymond Dils 1
PMCID: PMC1168093  PMID: 4455214

Abstract

1. A new assay system is described for monoglyceride acyltransferase (acylglycerol palmitoyltransferase, EC 2.3.1.22) in which palmitoyl-CoA is generated from palmitoyl-(−)-carnitine. 2. With the microsomal fraction from homogenates of guinea-pig intestinal mucosa, the Vmax. of this enzyme decreased with different acyl acceptors in the order 2-monopalmitoylglycerol>2-hexadecylglycerol>rac-1-monopalmitoylglycerol. 3. There were highly significant correlations between the monoglyceride acyltransferase activity as measured with these three substrates. This demonstrates that each of these substrates can be used to measure the same enzyme activity. 4. The advantages of using generated palmitoyl-CoA with 2-hexadecylglycerol and rac-1-monopalmitoylglycerol as model substrates for this enzyme are discussed.

Full text

PDF
410

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brindley D. N., Hübscher G. The intracellular distribution of the enzymes catalysing the biosynthesis of glycerides in the intestinal mucosa. Biochim Biophys Acta. 1965 Dec 2;106(3):495–509. doi: 10.1016/0005-2760(65)90066-4. [DOI] [PubMed] [Google Scholar]
  2. Brindley D. N. The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate by the microsomal fraction of guinea-pig intestinal mucosa. Biochem J. 1973 Apr;132(4):707–715. doi: 10.1042/bj1320707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Daae L. N., Bremer J. The acylation of glycerophosphate in rat liver. A new assay procedure for glycerophosphate acylation, studies on its subcellular and submitochondrial localization and determination of the reaction products. Biochim Biophys Acta. 1970 Jun 9;210(1):92–104. doi: 10.1016/0005-2760(70)90065-2. [DOI] [PubMed] [Google Scholar]
  4. Hübscher G., West G. R., Brindley D. N. Studies on the fractionation of mucosal homogenates from the small intestine. Biochem J. 1965 Dec;97(3):629–642. doi: 10.1042/bj0970629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kuhn N. J. Esterification of glycerol 3-phosphate in lactating guinea-pig mammary gland. Biochem J. 1967 Oct;105(1):213–223. doi: 10.1042/bj1050213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mangiapane E. H., Lloyd-Davies K. A., Brindley D. N. A study of some enzymes of glycerolipid biosynthesis in rat liver after subtotal hepatectomy. Biochem J. 1973 May;134(1):103–112. doi: 10.1042/bj1340103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Paltauf F., Johnston J. M. The metabolism in vitro of enantiomeric I-O-alkyl glycerols and I,2-and I,3-alkyl acyl glycerols in the intestinal mucosa. Biochim Biophys Acta. 1971 Jun 8;239(1):47–56. doi: 10.1016/0005-2760(71)90191-3. [DOI] [PubMed] [Google Scholar]
  8. Rao G. A., Johnston J. M. Purification and properties of triglyceride synthetase from the intestinal mucosa. Biochim Biophys Acta. 1966 Dec 7;125(3):465–473. doi: 10.1016/0005-2760(66)90035-x. [DOI] [PubMed] [Google Scholar]
  9. Rodgers J. B., Jr Assay of acyl-CoA:monoglyceride acyltransferase from rat small intestine using continuous recording spectrophotometry. J Lipid Res. 1969 Jul;10(4):427–432. [PubMed] [Google Scholar]
  10. Schultz F. M., Johnston J. M. The synthesis of higher glycerides via the monoglyceride pathway in hamster adipose tissue. J Lipid Res. 1971 Mar;12(2):132–138. [PubMed] [Google Scholar]
  11. Sánchez M., Nicholls D. G., Brindley D. N. [The relationship between palmitoyl-coenzyme A synthetase activity and esterification of sn-glycerol 3-phosphate in rat liver mitochondria]. Biochem J. 1973 Apr;132(4):697–706. doi: 10.1042/bj1320697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wood R., Snyder F. Chemical and physical properties of isomeric glyceryl monoethers. Lipids. 1967 Mar;2(2):161–171. doi: 10.1007/BF02530917. [DOI] [PubMed] [Google Scholar]
  13. Zahler W. L., Cleland W. W. Studies on the microsomal acylation of L-glycerol-3-phosphate. 3. Time course of the reaction. Biochim Biophys Acta. 1969 Jun 10;176(4):699–703. doi: 10.1016/0005-2760(69)90250-1. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES