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. 1974 Aug;141(2):413–418. doi: 10.1042/bj1410413

Purification and subunit structure of legumin of Vicia faba L. (broad bean)

David J Wright 1, Donald Boulter 1
PMCID: PMC1168094  PMID: 4455215

Abstract

Zonal isoelectric precipitation was shown to be an effective method for the preparation of legumin which was homogeneous as judged by ultracentrifugation and polyacrylamide-gel electrophoresis. The subunit structure of legumin was investigated by preparative sodium dodecyl sulphate–polyacrylamide-gel electrophoresis and ion-exchange chromatography in urea. Five distinct subunits, of which two were acidic (α) and had a molecular weight of 37000, and three were basic (β) with molecular weights of 20100, 20900 and 23800, were identified. The α and β subunits were present in equimolar amounts in the legumin molecule and, in view of this and molecular-weight considerations, an α6β6 subunit model was proposed for legumin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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