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. 1974 Sep;141(3):745–752. doi: 10.1042/bj1410745

The formation of iron-binding fragments of hen ovotransferrin by limited proteolysis

John Williams 1
PMCID: PMC1168181  PMID: 4463961

Abstract

1. Iron was added to hen ovotransferrin to 30% saturation and the protein was digested with trypsin or chymotrypsin. 2. Iron-binding fragments were isolated. They carried one atom of iron/mol (mol.wt. 35000) and consisted of a single polypeptide chain derived from the N-terminal half of the protein. Carbohydrate was not present. 3. The fragments were able to bind a variety of metals and to donate iron to reticulocytes.

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Selected References

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