Abstract
A technique has been described which permits the maintenance for up to 6 days in organ culture of explanted thyroid glands. This provided a suitable model for an electron microscope study to be carried out on glands under experimental conditions. The appearance of the control gland was consistent with stimulation by TSH. The formation of 'intracytoplasmic' follicles was caused by injection of TSH into the culture. Parafollicular (light) cells were described for the first time in organ cultured thyroid glands.
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- Baker T. G., Neal P. The effects of x-irradiation on mammalian oocytes in organ culture. Biophysik. 1969;6(1):39–45. doi: 10.1007/BF01403867. [DOI] [PubMed] [Google Scholar]
- Baker T. G., Young B. A. Fine structure of gonadotrophs and prolactin producing cells in the rat pars distalis in organ culture. J Anat. 1979 Dec;129(Pt 4):759–767. [PMC free article] [PubMed] [Google Scholar]
- Baker T. G., Young B. A. Organ culture of the rat thyroid gland. Experientia. 1973 Dec;29(12):1548–1550. doi: 10.1007/BF01943911. [DOI] [PubMed] [Google Scholar]
- Fujita H. Fine structure of the thyroid gland. Int Rev Cytol. 1975;40:197–280. doi: 10.1016/s0074-7696(08)60954-7. [DOI] [PubMed] [Google Scholar]
- THOMPSON B., ISLER H., SARKAR S. K. Effect of hypophysectomy and growth hormone on the light cells of the thyroid gland. Endocrinology. 1962 Jun;70:786–795. doi: 10.1210/endo-70-6-786. [DOI] [PubMed] [Google Scholar]
- WISSIG S. L. The anatomy of secretion in the follicular cells of the thyroid gland. I. The fine structure of the gland in the normal rat. J Biophys Biochem Cytol. 1960 Jun;7:419–432. doi: 10.1083/jcb.7.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YOSHIMURA F., YONETSU T., NAKAMURA M. Hormonal regulation of parafollicular cell in thyroid gland. Endocrinol Jpn. 1962 Dec;9:284–301. doi: 10.1507/endocrj1954.9.284. [DOI] [PubMed] [Google Scholar]
- YOUNG B. A., LEBLOND C. P. THE LIGHT CELL AS COMPARED TO THE FOLLICULAR CELL IN THE THYROID GLAND OF THE RAT. Endocrinology. 1963 Dec;73:669–686. doi: 10.1210/endo-73-6-669. [DOI] [PubMed] [Google Scholar]
- Yoshimura F., Yachi H., Ishikawa H., Otsuka Y., Kiguchi Y. Morphological and functional differentiation of isolated parafollicular and follicular epithelial cells of the rat thyroids during culture. Endocrinol Jpn. 1972 Apr;19(2):151–161. doi: 10.1507/endocrj1954.19.151. [DOI] [PubMed] [Google Scholar]
- Young B. A. Intercellular channels in the canine and porcine thyroid gland. J Anat. 1966 Oct;100(Pt 4):895–898. [PMC free article] [PubMed] [Google Scholar]