Abstract
1. Ricin, a toxic protein from the seeds of Ricinus communis which inhibits poly(U)-directed polyphenylalanine synthesis by rat liver ribosomes (Montanaro et al., 1973), does not affect protein synthesis by isolated rat liver mitochondria. 2. The toxin is ineffective also on poly(U)-directed polyphenylalanine synthesis in reconstituted systems with ribosomes isolated from rat liver mitochondria or from Escherichia coli. 3. Ricin inhibits protein synthesis by isolated rat liver nuclei, but at concentrations much higher than those affecting rat liver ribosomes.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Greco M., Cantatore P., Pepe G., Saccone C. Isolation and characterization of rat-liver mitochondrial ribosomes highly active in poly(U)-directed polyphenylalanine synthesis. Eur J Biochem. 1973 Aug 1;37(1):171–177. doi: 10.1111/j.1432-1033.1973.tb02972.x. [DOI] [PubMed] [Google Scholar]
- Laval M., Bouteille M. Synthetic activity of isolated rat liver nuclei. I. Ultrastructural study at various steps of isolation. Exp Cell Res. 1973 Feb;76(2):337–348. doi: 10.1016/0014-4827(73)90385-6. [DOI] [PubMed] [Google Scholar]
- Laval M., Bouteille M. Synthetic activity of isolated rat liver nuclei. II. Evidence for protein formation. Exp Cell Res. 1973 Jun;79(2):391–403. doi: 10.1016/0014-4827(73)90459-x. [DOI] [PubMed] [Google Scholar]
- Montanaro L., Sperti S., Stirpe F. Inhibition by ricin of protein synthesis in vitro. Ribosomes as the target of the toxin. Biochem J. 1973 Nov;136(3):677–683. doi: 10.1042/bj1360677. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NIRENBERG M. W., MATTHAEI J. H. The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1588–1602. doi: 10.1073/pnas.47.10.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Olsnes S., Pihl A. Ricin - a potent inhibitor of protein synthesis. FEBS Lett. 1972 Feb 15;20(3):327–329. doi: 10.1016/0014-5793(72)80098-x. [DOI] [PubMed] [Google Scholar]
- Richter D., Lipmann F. Separation of mitochondrial and cytoplasmic peptide chain elongation factors from yeast. Biochemistry. 1970 Dec 22;9(26):5065–5070. doi: 10.1021/bi00828a004. [DOI] [PubMed] [Google Scholar]
- Saccone C., Gadaleta M. N., Gallerani R. RNA synthesis in isolated rat liver mitochondria. Eur J Biochem. 1969 Aug;10(1):61–65. doi: 10.1111/j.1432-1033.1969.tb00655.x. [DOI] [PubMed] [Google Scholar]
- Sperti S., Montanaro L., Mattioli A., Stirpe F. Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin. Biochem J. 1973 Nov;136(3):813–815. doi: 10.1042/bj1360813. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOOD W. B., BERG P. The effect of enzymatically synthesized ribonucleic acid on amino acid incorporation by a soluble protein-ribosome system from Escherichia coli. Proc Natl Acad Sci U S A. 1962 Jan 15;48:94–104. doi: 10.1073/pnas.48.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]