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. 1998 Sep 1;17(17):5161–5169. doi: 10.1093/emboj/17.17.5161

TAFII105 mediates activation of anti-apoptotic genes by NF-kappaB.

A Yamit-Hezi 1, R Dikstein 1
PMCID: PMC1170844  PMID: 9724652

Abstract

The transcription factor NF-kappaB is important for expression of genes involved in immune responses, viral infections, cytokine signaling and stress. In addition NF-kappaB plays a crucial role in protecting cells from TNF-alpha-induced apoptotic stimuli, presumably by activating anti-apoptotic genes. Here we report that the sub-stoichiometric TFIID subunit TAFII105 is essential for activation of anti-apoptotic genes in response to TNF-alpha, serving as a transcriptional coactivator for NF-kappaB. The putative coactivator domain of TAFII105 interacts with the activation domain of the p65/RelA member of the NF-kappaB family, and further stimulates p65-induced transcription in human 293 cells. Moreover, inhibition of TAFII105 activity by overexpression of a dominant negative mutant of TAFII105 decreased NF-kappaB transcriptional activity and severely reduced cell survival in response to TNF-alpha. Similarly, expression of anti-sense TAFII105 RNA sensitized the cells to TNF-alpha cytotoxicity. These results suggest that TAFII105 is involved in activation of anti-apoptotic genes by NF-kappaB.

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Selected References

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  1. Altschmied J., Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. Biotechniques. 1997 Sep;23(3):436–438. doi: 10.2144/97233bm19. [DOI] [PubMed] [Google Scholar]
  2. Attardi L. D., Tjian R. Drosophila tissue-specific transcription factor NTF-1 contains a novel isoleucine-rich activation motif. Genes Dev. 1993 Jul;7(7B):1341–1353. doi: 10.1101/gad.7.7b.1341. [DOI] [PubMed] [Google Scholar]
  3. Baeuerle P. A., Baltimore D. NF-kappa B: ten years after. Cell. 1996 Oct 4;87(1):13–20. doi: 10.1016/s0092-8674(00)81318-5. [DOI] [PubMed] [Google Scholar]
  4. Baldwin A. S., Jr The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol. 1996;14:649–683. doi: 10.1146/annurev.immunol.14.1.649. [DOI] [PubMed] [Google Scholar]
  5. Beg A. A., Baltimore D. An essential role for NF-kappaB in preventing TNF-alpha-induced cell death. Science. 1996 Nov 1;274(5288):782–784. doi: 10.1126/science.274.5288.782. [DOI] [PubMed] [Google Scholar]
  6. Brunvand M. W., Krumm A., Groudine M. In vivo footprinting of the human IL-2 gene reveals a nuclear factor bound to the transcription start site in T cells. Nucleic Acids Res. 1993 Oct 11;21(20):4824–4829. doi: 10.1093/nar/21.20.4824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chen J. L., Attardi L. D., Verrijzer C. P., Yokomori K., Tjian R. Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activators. Cell. 1994 Oct 7;79(1):93–105. doi: 10.1016/0092-8674(94)90403-0. [DOI] [PubMed] [Google Scholar]
  8. Chiang C. M., Roeder R. G. Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators. Science. 1995 Jan 27;267(5197):531–536. doi: 10.1126/science.7824954. [DOI] [PubMed] [Google Scholar]
  9. Chu Z. L., McKinsey T. A., Liu L., Gentry J. J., Malim M. H., Ballard D. W. Suppression of tumor necrosis factor-induced cell death by inhibitor of apoptosis c-IAP2 is under NF-kappaB control. Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10057–10062. doi: 10.1073/pnas.94.19.10057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dikstein R., Zhou S., Tjian R. Human TAFII 105 is a cell type-specific TFIID subunit related to hTAFII130. Cell. 1996 Oct 4;87(1):137–146. doi: 10.1016/s0092-8674(00)81330-6. [DOI] [PubMed] [Google Scholar]
  11. Dynlacht B. D., Hoey T., Tjian R. Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation. Cell. 1991 Aug 9;66(3):563–576. doi: 10.1016/0092-8674(81)90019-2. [DOI] [PubMed] [Google Scholar]
  12. Goodrich J. A., Hoey T., Thut C. J., Admon A., Tjian R. Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB. Cell. 1993 Nov 5;75(3):519–530. doi: 10.1016/0092-8674(93)90386-5. [DOI] [PubMed] [Google Scholar]
  13. Hoey T., Weinzierl R. O., Gill G., Chen J. L., Dynlacht B. D., Tjian R. Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators. Cell. 1993 Jan 29;72(2):247–260. doi: 10.1016/0092-8674(93)90664-c. [DOI] [PubMed] [Google Scholar]
  14. Hoffmann A., Oelgeschläger T., Roeder R. G. Considerations of transcriptional control mechanisms: do TFIID-core promoter complexes recapitulate nucleosome-like functions? Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):8928–8935. doi: 10.1073/pnas.94.17.8928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Horikoshi M., Hai T., Lin Y. S., Green M. R., Roeder R. G. Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complex. Cell. 1988 Sep 23;54(7):1033–1042. doi: 10.1016/0092-8674(88)90118-3. [DOI] [PubMed] [Google Scholar]
  16. Jacq X., Brou C., Lutz Y., Davidson I., Chambon P., Tora L. Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor. Cell. 1994 Oct 7;79(1):107–117. doi: 10.1016/0092-8674(94)90404-9. [DOI] [PubMed] [Google Scholar]
  17. Karsan A., Yee E., Harlan J. M. Endothelial cell death induced by tumor necrosis factor-alpha is inhibited by the Bcl-2 family member, A1. J Biol Chem. 1996 Nov 1;271(44):27201–27204. doi: 10.1074/jbc.271.44.27201. [DOI] [PubMed] [Google Scholar]
  18. Künzler M., Braus G. H., Georgiev O., Seipel K., Schaffner W. Functional differences between mammalian transcription activation domains at the yeast GAL1 promoter. EMBO J. 1994 Feb 1;13(3):641–645. doi: 10.1002/j.1460-2075.1994.tb06302.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Liu Z. G., Hsu H., Goeddel D. V., Karin M. Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-kappaB activation prevents cell death. Cell. 1996 Nov 1;87(3):565–576. doi: 10.1016/s0092-8674(00)81375-6. [DOI] [PubMed] [Google Scholar]
  20. May M., Mengus G., Lavigne A. C., Chambon P., Davidson I. Human TAF(II28) promotes transcriptional stimulation by activation function 2 of the retinoid X receptors. EMBO J. 1996 Jun 17;15(12):3093–3104. [PMC free article] [PubMed] [Google Scholar]
  21. Mengus G., May M., Carré L., Chambon P., Davidson I. Human TAF(II)135 potentiates transcriptional activation by the AF-2s of the retinoic acid, vitamin D3, and thyroid hormone receptors in mammalian cells. Genes Dev. 1997 Jun 1;11(11):1381–1395. doi: 10.1101/gad.11.11.1381. [DOI] [PubMed] [Google Scholar]
  22. Miyamoto S., Verma I. M. Rel/NF-kappa B/I kappa B story. Adv Cancer Res. 1995;66:255–292. [PubMed] [Google Scholar]
  23. Moore P. A., Ruben S. M., Rosen C. A. Conservation of transcriptional activation functions of the NF-kappa B p50 and p65 subunits in mammalian cells and Saccharomyces cerevisiae. Mol Cell Biol. 1993 Mar;13(3):1666–1674. doi: 10.1128/mcb.13.3.1666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moqtaderi Z., Bai Y., Poon D., Weil P. A., Struhl K. TBP-associated factors are not generally required for transcriptional activation in yeast. Nature. 1996 Sep 12;383(6596):188–191. doi: 10.1038/383188a0. [DOI] [PubMed] [Google Scholar]
  25. Opipari A. W., Jr, Hu H. M., Yabkowitz R., Dixit V. M. The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity. J Biol Chem. 1992 Jun 25;267(18):12424–12427. [PubMed] [Google Scholar]
  26. Orphanides G., Lagrange T., Reinberg D. The general transcription factors of RNA polymerase II. Genes Dev. 1996 Nov 1;10(21):2657–2683. doi: 10.1101/gad.10.21.2657. [DOI] [PubMed] [Google Scholar]
  27. Ponticelli A. S., Pardee T. S., Struhl K. The glutamine-rich activation domains of human Sp1 do not stimulate transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1995 Feb;15(2):983–988. doi: 10.1128/mcb.15.2.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Poon D., Weil P. A. Immunopurification of yeast TATA-binding protein and associated factors. Presence of transcription factor IIIB transcriptional activity. J Biol Chem. 1993 Jul 25;268(21):15325–15328. [PubMed] [Google Scholar]
  29. Pugh B. F., Tjian R. Mechanism of transcriptional activation by Sp1: evidence for coactivators. Cell. 1990 Jun 29;61(7):1187–1197. doi: 10.1016/0092-8674(90)90683-6. [DOI] [PubMed] [Google Scholar]
  30. Reese J. C., Apone L., Walker S. S., Griffin L. A., Green M. R. Yeast TAFIIS in a multisubunit complex required for activated transcription. Nature. 1994 Oct 6;371(6497):523–527. doi: 10.1038/371523a0. [DOI] [PubMed] [Google Scholar]
  31. Sauer F., Hansen S. K., Tjian R. DNA template and activator-coactivator requirements for transcriptional synergism by Drosophila bicoid. Science. 1995 Dec 15;270(5243):1825–1828. doi: 10.1126/science.270.5243.1825. [DOI] [PubMed] [Google Scholar]
  32. Sauer F., Hansen S. K., Tjian R. Multiple TAFIIs directing synergistic activation of transcription. Science. 1995 Dec 15;270(5243):1783–1788. doi: 10.1126/science.270.5243.1783. [DOI] [PubMed] [Google Scholar]
  33. Schmitz M. L., Baeuerle P. A. Multi-step activation of NF-kappa B/Rel transcription factors. Immunobiology. 1995 Jul;193(2-4):116–127. doi: 10.1016/s0171-2985(11)80534-6. [DOI] [PubMed] [Google Scholar]
  34. Schmitz M. L., Baeuerle P. A. The p65 subunit is responsible for the strong transcription activating potential of NF-kappa B. EMBO J. 1991 Dec;10(12):3805–3817. doi: 10.1002/j.1460-2075.1991.tb04950.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Suzuki-Yagawa Y., Guermah M., Roeder R. G. The ts13 mutation in the TAF(II)250 subunit (CCG1) of TFIID directly affects transcription of D-type cyclin genes in cells arrested in G1 at the nonpermissive temperature. Mol Cell Biol. 1997 Jun;17(6):3284–3294. doi: 10.1128/mcb.17.6.3284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Tanese N., Pugh B. F., Tjian R. Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex. Genes Dev. 1991 Dec;5(12A):2212–2224. doi: 10.1101/gad.5.12a.2212. [DOI] [PubMed] [Google Scholar]
  37. Tanese N., Saluja D., Vassallo M. F., Chen J. L., Admon A. Molecular cloning and analysis of two subunits of the human TFIID complex: hTAFII130 and hTAFII100. Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13611–13616. doi: 10.1073/pnas.93.24.13611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Tansey W. P., Herr W. TAFs: guilt by association? Cell. 1997 Mar 21;88(6):729–732. doi: 10.1016/s0092-8674(00)81916-9. [DOI] [PubMed] [Google Scholar]
  39. Thut C. J., Chen J. L., Klemm R., Tjian R. p53 transcriptional activation mediated by coactivators TAFII40 and TAFII60. Science. 1995 Jan 6;267(5194):100–104. doi: 10.1126/science.7809597. [DOI] [PubMed] [Google Scholar]
  40. Van Antwerp D. J., Martin S. J., Kafri T., Green D. R., Verma I. M. Suppression of TNF-alpha-induced apoptosis by NF-kappaB. Science. 1996 Nov 1;274(5288):787–789. doi: 10.1126/science.274.5288.787. [DOI] [PubMed] [Google Scholar]
  41. Verma I. M., Stevenson J. K., Schwarz E. M., Van Antwerp D., Miyamoto S. Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation. Genes Dev. 1995 Nov 15;9(22):2723–2735. doi: 10.1101/gad.9.22.2723. [DOI] [PubMed] [Google Scholar]
  42. Verrijzer C. P., Tjian R. TAFs mediate transcriptional activation and promoter selectivity. Trends Biochem Sci. 1996 Sep;21(9):338–342. [PubMed] [Google Scholar]
  43. Walker S. S., Reese J. C., Apone L. M., Green M. R. Transcription activation in cells lacking TAFIIS. Nature. 1996 Sep 12;383(6596):185–188. doi: 10.1038/383185a0. [DOI] [PubMed] [Google Scholar]
  44. Wang C. Y., Mayo M. W., Baldwin A. S., Jr TNF- and cancer therapy-induced apoptosis: potentiation by inhibition of NF-kappaB. Science. 1996 Nov 1;274(5288):784–787. doi: 10.1126/science.274.5288.784. [DOI] [PubMed] [Google Scholar]
  45. Wang E. H., Zou S., Tjian R. TAFII250-dependent transcription of cyclin A is directed by ATF activator proteins. Genes Dev. 1997 Oct 15;11(20):2658–2669. doi: 10.1101/gad.11.20.2658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Wong G. H., Elwell J. H., Oberley L. W., Goeddel D. V. Manganous superoxide dismutase is essential for cellular resistance to cytotoxicity of tumor necrosis factor. Cell. 1989 Sep 8;58(5):923–931. doi: 10.1016/0092-8674(89)90944-6. [DOI] [PubMed] [Google Scholar]
  47. Zhou Q., Lieberman P. M., Boyer T. G., Berk A. J. Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter. Genes Dev. 1992 Oct;6(10):1964–1974. doi: 10.1101/gad.6.10.1964. [DOI] [PubMed] [Google Scholar]

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