Abstract
Increasing concentrations of CDP-choline progressively inhibit the measured incorporation of CDP-[2-14C]ethanolamine into phosphatidylethanolamine catalysed by the ethanolaminephosphotransferase present in endoplasmic-reticulum membranes isolated from castor-bean endosperm cells. This inhibition parallels that observed during CDP-[Me-14C]choline incorporation and suggests that a single enzyme utilizes both these substrates.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bowden L., Lord J. M. Development of phospholipid synthesizing enzymes in castor bean endosperm. FEBS Lett. 1975 Jan 1;49(3):369–371. doi: 10.1016/0014-5793(75)80787-3. [DOI] [PubMed] [Google Scholar]
- CHOJNACKI T. ON THE IN VITRO FORMATION OF PHOSPHOLIPIDS CONTAINING UNNATURAL BASES, VIA THE CYTIDINE MECHANISM. Acta Biochim Pol. 1964;11:11–23. [PubMed] [Google Scholar]
- Donaldson R. P., Tolbert N. E., Schnarrenberger C. A comparison of microbody membranes with microsomes and mitochondria from plant and animal tissue. Arch Biochem Biophys. 1972 Sep;152(1):199–215. doi: 10.1016/0003-9861(72)90208-1. [DOI] [PubMed] [Google Scholar]
- Haubrich D. R. Partial purification and properties of choline kinase (EC 2. 7. 1. 32) from rabbit brain: measurement of acetylcholine. J Neurochem. 1973 Aug;21(2):315–328. doi: 10.1111/j.1471-4159.1973.tb04252.x. [DOI] [PubMed] [Google Scholar]
- KENNEDY E. P. Biosynthesis of complex lipids. Fed Proc. 1961 Dec;20:934–940. [PubMed] [Google Scholar]
- KENNEDY E. P. The synthesis of cytidine diphosphate choline, cytidine diphosphate ethanolamine, and related compounds. J Biol Chem. 1956 Sep;222(1):185–191. [PubMed] [Google Scholar]
- Lord J. M., Kagawa T., Beevers H. Intracellular distribution of enzymes of the cytidine diphosphate choline pathway in castor bean endosperm. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2429–2432. doi: 10.1073/pnas.69.9.2429. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lord J. M., Kagawa T., Moore T. S., Beevers H. Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J Cell Biol. 1973 Jun;57(3):659–667. doi: 10.1083/jcb.57.3.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macher B. A., Mudd J. B. Biosynthesis of phosphatidylethanolamine by enzyme preparations from plant tissues. Plant Physiol. 1974 Feb;53(2):171–175. doi: 10.1104/pp.53.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILGRAM G. F., KENNEDY E. P. INTRACELLULAR DISTRIBUTION OF SOME ENZYMES CATALYZING REACTIONS IN THE BIOSYNTHESIS OF COMPLEX LIPIDS. J Biol Chem. 1963 Aug;238:2615–2619. [PubMed] [Google Scholar]
- WITTENBERG J., KORNBERG A. Choline phosphokinase. J Biol Chem. 1953 May;202(1):431–444. [PubMed] [Google Scholar]