Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1975 Dec;151(3):469–475. doi: 10.1042/bj1510469a

Immunological characterization of human liver alpha-D-mannosidase.

N Phillips, D Robinson, B Winchester
PMCID: PMC1172393  PMID: 814895

Abstract

Antiserum was raised against purified human liver alpha-D-mannosidase B. It precipitated alpha-mannosidases A and B from solution, demonstrating the close structural resemblance of these 2 forms of acidic alpha-mannosidase activity. A continuous enzymically active precipitin line with no spurs was obtained when alpha-mannosidase A and B were placed in adjacent wells on Ouchterlony double-diffusion plates. The antiserum precipitated acidic but not neutral alpha-mannosidase from an extract of human liver, confirming that the acidic and neutral activities are not closely related. Acidic activity was also precipitated from extracts of human brain, kidney and leucocytes by the antiserum. However, it did not cross-react with bovine acidic alpha-mannosidase activity or with the activity in human plasma that has an optimum pH of 5.5. The two acidic forms of human liver alpha-mannosidase, A and B, are immunologically identical but distinct from neutral alpha-mannosidase and that activity with an optimum pH of 5.5.

Full text

PDF
475

Images in this article

474-1PLATE 1
on p.474-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beutler E., Kuhl W. Purification and properties of human alpha-galactosidases. J Biol Chem. 1972 Nov 25;247(22):7195–7200. [PubMed] [Google Scholar]
  2. Braidman I., Carroll M., Dance N., Robinson D., Poenaru L., Weber A., Dreyfus J. C., Overdijk B., Hooghwinkel G. J. Characterisation of human N-acetyl-beta-hexosaminidase C. FEBS Lett. 1974 May 1;41(2):181–184. doi: 10.1016/0014-5793(74)81206-8. [DOI] [PubMed] [Google Scholar]
  3. Carroll M., Dance N., Masson P. K., Robinson D., Winchester B. G. Human mannosidosis--the enzyme defect. Biochem Biophys Res Commun. 1972 Oct 17;49(2):579–583. doi: 10.1016/0006-291x(72)90450-0. [DOI] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Dingle J. T., Barrett A. J., Weston P. D. Cathepsin D. Characteristics of immunoinhibition and the confirmation of a role in cartilage breakdown. Biochem J. 1971 Jun;123(1):1–13. doi: 10.1042/bj1230001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hocking J. D., Jolly R. D., Batt R. D. Deficiency of alpha-mannosidase in Angus cattle. An inherited lysosomal storage disease. Biochem J. 1972 Jun;128(1):69–78. doi: 10.1042/bj1280069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LEABACK D. H., WALKER P. G. Studies on glucosaminidase. 4. The fluorimetric assay of N-acetyl-beta-glucosaminidase. Biochem J. 1961 Jan;78:151–156. doi: 10.1042/bj0780151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Meisler M., Rattazzi M. C. Immunological studies of beta galactosidase in normal human liver and in GM1 gangliosidosis. Am J Hum Genet. 1974 Nov;26(6):683–691. [PMC free article] [PubMed] [Google Scholar]
  9. Neuwelt E., Stumpf D., Austin J., Kohler P. A monospecific antibody to human sulfatase A. Preparation, characterization and significance. Biochim Biophys Acta. 1971 Apr 27;236(1):333–346. doi: 10.1016/0005-2795(71)90182-6. [DOI] [PubMed] [Google Scholar]
  10. Norden A. G., Tennant L. L., O'Brien J. S. GM1 ganglioside beta-galactosidase. A. Purification and studies of the enzyme from human liver. J Biol Chem. 1974 Dec 25;249(24):7969–7976. [PubMed] [Google Scholar]
  11. POLLOCK M. R. Penicillinase-antipenicillinase. Ann N Y Acad Sci. 1963 May 8;103:989–1005. doi: 10.1111/j.1749-6632.1963.tb53750.x. [DOI] [PubMed] [Google Scholar]
  12. Phillips N. C., Robinson D., Winchester B. G. Human liver alpha-D-mannosidase activity. Clin Chim Acta. 1974 Aug 30;55(1):11–19. doi: 10.1016/0009-8981(74)90328-3. [DOI] [PubMed] [Google Scholar]
  13. Phillips N. C., Robinson D., Winchester B. G. Immunological characterization of human alpha-D-mannosidases. Biochem Soc Trans. 1975;3(2):238–240. doi: 10.1042/bst0030238. [DOI] [PubMed] [Google Scholar]
  14. Phillips N. C., Robinson D., Winchester B. G., Jolly R. D. Mannosidosis in Angus cattle. The enzymic defect. Biochem J. 1974 Feb;137(2):363–371. doi: 10.1042/bj1370363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Robinson D., Thorpe R. Fluorescent assay of alpha-L-fucosidase. Clin Chim Acta. 1974 Aug 30;55(1):65–69. doi: 10.1016/0009-8981(74)90334-9. [DOI] [PubMed] [Google Scholar]
  16. Srivastava S. K., Beutler E. Antibody against purified human hexosaminidase B cross-reacting with human hexosaminidase A. Biochem Biophys Res Commun. 1972 May 26;47(4):753–759. doi: 10.1016/0006-291x(72)90556-6. [DOI] [PubMed] [Google Scholar]
  17. Vesterberg O., Svensson H. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins. Acta Chem Scand. 1966;20(3):820–834. doi: 10.3891/acta.chem.scand.20-0820. [DOI] [PubMed] [Google Scholar]
  18. de Barsy T., Jacquemin P., Devos P., Hers H. G. Rodent and human acid -glucosidase. Purification, properties and inhibition by antibodies. Investigation in type II glycogenosis. Eur J Biochem. 1972 Nov 21;31(1):156–165. doi: 10.1111/j.1432-1033.1972.tb02514.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES