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. 1976 Jan 1;153(1):75–78. doi: 10.1042/bj1530075

Carbohydrate composition of lymphocyte plasma membrane from pig mesenteric lymph node.

D Snary, A K Allen, R A Faulkes, M J Crumpton
PMCID: PMC1172542  PMID: 1259716

Abstract

Pig lymphocyte plasma membrane isolated from mesenteric lymph node contained 69 mug of carbohydrate/mg dry wt., which was made up of neutral sugar, amino sugar and sialic acid in the molar proportions 5:1.7:1. The neutral sugar comprised fucose, ribose, mannose, glucose, galactose and inositol (molar proportions 2:9:11:15:26:1), and the amino sugar glucosamine and galactosamine (molar ratio 2:1). The ribose was most probably derived from RNA. All of the fucose and mannose and almost all of the glucosamine were associated with the membrane protein whereas the membrane lipid contained all of the inositol. The remaining sugars were distributed in various ratios between the protein and lipid fractions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allan D., Crumpton M. J. Preparation and characterization of the plasma membrane of pig lymphocytes. Biochem J. 1970 Nov;120(1):133–143. doi: 10.1042/bj1200133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allen A. K., Neuberger A. The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein. Biochem J. 1973 Oct;135(2):307–314. doi: 10.1042/bj1350307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barber A. J., Jamieson G. A. Isolation and characterization of plasma membranes from human blood platelets. J Biol Chem. 1970 Dec 10;245(23):6357–6365. [PubMed] [Google Scholar]
  4. Clamp J. R. Analysis of glycoproteins. Biochem Soc Symp. 1974;(40):3–16. [PubMed] [Google Scholar]
  5. Droege W., Strominger J. L., Singh P. P., Lüderitz O. Surface carbohydrate composition of different types of chicken lymphocytes. Eur J Biochem. 1975 May;54(1):301–306. doi: 10.1111/j.1432-1033.1975.tb04140.x. [DOI] [PubMed] [Google Scholar]
  6. Evans W. H., Gurd J. W. Biosynthesis of liver membranes. Incorporation of ( 3 H)leucine into proteins and of ( 14 C)glucosamine into proteins and lipids of liver microsomal and plasma-membrane fractions. Biochem J. 1971 Nov;125(2):615–624. doi: 10.1042/bj1250615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  8. Griggs L. J., Post A., White E. R., Finkelstein J. A., Moeckel W. E., Holden K. G., Zarembo J. E., Weisbach J. A. Identification and quantitation of alditol acetates of neutral and amino sugars from mucins by automated gas-liquid chromatography. Anal Biochem. 1971 Oct;43(2):369–381. doi: 10.1016/0003-2697(71)90266-1. [DOI] [PubMed] [Google Scholar]
  9. Hakomori S. Glycolipids of tumor cell membrane. Adv Cancer Res. 1973;18:265–315. doi: 10.1016/s0065-230x(08)60755-1. [DOI] [PubMed] [Google Scholar]
  10. Jourdian G. W., Dean L., Roseman S. The sialic acids. XI. A periodate-resorcinol method for the quantitative estimation of free sialic acids and their glycosides. J Biol Chem. 1971 Jan 25;246(2):430–435. [PubMed] [Google Scholar]
  11. KLENK E., UHLENBRUCK G. Uber ein neuraminsäurehaltiges Mucoproteid aus Rindererythrocytenstroma. Hoppe Seylers Z Physiol Chem. 1958;311(4-6):227–233. [PubMed] [Google Scholar]
  12. Kim Y. S., Perdomo J. M. Membrane glycoproteins of the rat small intestine. Chemical composition of membrane glycoproteins. Biochim Biophys Acta. 1974 Mar 14;342(1):111–124. doi: 10.1016/0005-2795(74)90112-3. [DOI] [PubMed] [Google Scholar]
  13. Nicolson G. L. The interactions of lectins with animal cell surfaces. Int Rev Cytol. 1974;39:89–190. doi: 10.1016/s0074-7696(08)60939-0. [DOI] [PubMed] [Google Scholar]
  14. Quirk S. J., Robinson G. B. Isolation and characterization of rabbit kidney brush borders. Biochem J. 1972 Aug;128(5):1319–1328. doi: 10.1042/bj1281319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roth S. A molecular model for cell interactions. Q Rev Biol. 1973 Dec;48(4):541–563. doi: 10.1086/407816. [DOI] [PubMed] [Google Scholar]
  16. Shimizu S., Funakoshi I. Carbohydrate composition of the plasma membranes of rat ascites hepatoma. Biochim Biophys Acta. 1970 Mar 17;203(1):167–169. doi: 10.1016/0005-2736(70)90046-5. [DOI] [PubMed] [Google Scholar]
  17. Steck T. L., Dawson G. Topographical distribution of complex carbohydrates in the erythrocyte membrane. J Biol Chem. 1974 Apr 10;249(7):2135–2142. [PubMed] [Google Scholar]

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