Abstract
The inhibition of lactose synthesis by UDP-glucose, UDP-glucuronate and, less so, by UDP-N-acetylglucosamine was markedly smaller in preparations of "intact" than of lysed vesicles derived from the Golgi apparatus of lactating rat mammary gland. This constitutes evidence for a specific, probably facilitated, transport of UDP-galactose across the Golgi membrane.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Keppler D., Rudigier J., Decker K. Enzymic determination of uracil nucleotides in tissues. Anal Biochem. 1970 Nov;38(1):105–114. doi: 10.1016/0003-2697(70)90160-0. [DOI] [PubMed] [Google Scholar]
- Khatra B. S., Herries D. G., Brew K. Some kinetic properties of human-milk galactosyl transferase. Eur J Biochem. 1974 May 15;44(2):537–560. doi: 10.1111/j.1432-1033.1974.tb03513.x. [DOI] [PubMed] [Google Scholar]
- Kitchen B. J., Andrens P. Studies on the thiol group of lactose synthetase A protein from human milk and on the binding of uridine diphosphate galactose to the enzyme. Biochem J. 1974 Jul;141(1):173–178. doi: 10.1042/bj1410173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuhn N. J., White A. The topography of lactose synthesis. Biochem J. 1975 Apr;148(1):77–84. doi: 10.1042/bj1480077. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morrison J. F., Ebner K. E. Studies on galactosyltransferase. Kinetic investigations with glucose as the galactosyl group acceptor. J Biol Chem. 1971 Jun 25;246(12):3985–3991. [PubMed] [Google Scholar]
- Murphy G., Ariyanayagam A. D., Kuhn N. J. Progesterone and the metabolic control of the lactose biosynthetic pathway during lactogenesis in the rat. Biochem J. 1973 Dec;136(4):1105–1116. doi: 10.1042/bj1361105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILSON D. B., HOGNESS D. S. THE ENZYMES OF THE GALACTOSE OPERON IN ESCHERICHIA COLI. I. PURIFICATION AND CHARACTERIZATION OF URIDINE DIPHOSPHOGALACTOSE 4-EPIMERASE. J Biol Chem. 1964 Aug;239:2469–2481. [PubMed] [Google Scholar]