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. 1972 Jun;128(1):41–46. doi: 10.1042/bj1280041

The enzymic degradation of l-serine O-sulphate. Mechanism of the reaction

N Tudball 1, P Thomas 1,*
PMCID: PMC1173567  PMID: 4673572

Abstract

1. During the enzyme-catalysed degradation of l-serine O-sulphate no exchange occurs between the hydrogen atom attached to the α-carbon atom of the substrate and the tritiated water of the incubation medium. 2. The participation of an intermediate carbanion has been demonstrated in the degradation by performing the reaction in the presence of tetranitromethane. 3. Photo-oxidation of the enzyme in the presence of Rose Bengal led to a rapid inactivation of enzyme with the concomitant loss of four histidine residues/molecule. 4. Rose Bengal was also a non-competitive inhibitor of the enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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