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. 1972 Oct;129(4):969–976. doi: 10.1042/bj1290969

Enzyme-mediated binding of tyrosine to brome-mosaic-virus ribonucleic acid

T C Hall 1, D S Shih 1, Paul Kaesberg 1
PMCID: PMC1174243  PMID: 4655830

Abstract

Incubation of tyrosine with the RNA from brome-mosaic-virus under conditions favourable for aminoacylation of tRNA resulted in binding of tyrosine to the viral RNA. Sucrose-density-gradient centrifugation showed that tyrosine binds to three and probably to all four of the viral RNA components. The bound amino acid was readily released in mild alkaline solutions. Of 20 amino acids tested, only tyrosine was found to bind. The maximum extent of binding observed was 0.58mol of tyrosine/mol of brome-mosaic-virus RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bandyopadhyay A. K., Deutscher M. P. Complex of aminoacyl-transfer RNA synthetases. J Mol Biol. 1971 Aug 28;60(1):113–122. doi: 10.1016/0022-2836(71)90451-7. [DOI] [PubMed] [Google Scholar]
  2. Beljanski M. L'ARN isolé du virus de la mosaique jaune du navet; accepteur des L-acides aminés en présence d'enzymes bactériens. Bull Soc Chim Biol (Paris) 1965;47(8):1645–1652. [PubMed] [Google Scholar]
  3. Bockstahler L. E., Kaesberg P. Isolation and properties of RNA from bromegrass mosaic virus. J Mol Biol. 1965 Aug;13(1):127–137. doi: 10.1016/s0022-2836(65)80084-5. [DOI] [PubMed] [Google Scholar]
  4. Burkard G., Guillemaut P., Weil J. H. Comparative studies of the tRNA's and the aminoacyl-tRNA synthetases from the cytoplasm and the chloroplasts of Phaseolus vulgaris. Biochim Biophys Acta. 1970 Nov 12;224(1):184–198. doi: 10.1016/0005-2787(70)90632-5. [DOI] [PubMed] [Google Scholar]
  5. Glitz D. G., Eichler D. Nucleotides at the 5'-linked ends of bromegrass mosaic virus RNA and its fragments. Biochim Biophys Acta. 1971 May 13;238(2):224–232. doi: 10.1016/0005-2787(71)90089-x. [DOI] [PubMed] [Google Scholar]
  6. Guderian R. H., Pulliam R. L., Gordon M. P. Characterization and fractionation of tobacco leaf transfer RNA. Biochim Biophys Acta. 1972 Feb 23;262(1):50–65. doi: 10.1016/0005-2787(72)90218-3. [DOI] [PubMed] [Google Scholar]
  7. Hall T. C., Weiser C. J. Liquid scintillation counting at ambient temperature. Anal Biochem. 1966 Nov;17(2):294–299. doi: 10.1016/0003-2697(66)90207-7. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lane L. C., Kaesberg P. Multiple genetic components in bromegrass mosaic virus. Nat New Biol. 1971 Jul 14;232(28):40–43. doi: 10.1038/newbio232040a0. [DOI] [PubMed] [Google Scholar]
  10. Mitra S. K., Chakraburtty K., Mehler A. H. Binding of transfer RNA and arginine to the arginine transfer RNA synthetase of Escherichia coli. J Mol Biol. 1970 Apr 14;49(1):139–156. doi: 10.1016/0022-2836(70)90382-7. [DOI] [PubMed] [Google Scholar]
  11. Pinck M., Schuber F. Reaction of tris-(hydroxymethyl)-aminomethane with valyl-TYMV-RNA. Biochimie. 1971;53(8):887–891. doi: 10.1016/s0300-9084(71)80152-9. [DOI] [PubMed] [Google Scholar]
  12. Pinck M., Yot P., Chapeville F., Duranton H. M. Enzymatic binding of valine to the 3' end of TYMV-RNA. Nature. 1970 Jun 6;226(5249):954–956. doi: 10.1038/226954a0. [DOI] [PubMed] [Google Scholar]
  13. Rubin I. B., Kelmers A. D., Goldstein G. The determination of transfer ribonucleic acid by aminoacylation. I. Leucine and phenylalanine transfer ribonucleic acid from E. coli B. Anal Biochem. 1967 Sep;20(3):533–544. doi: 10.1016/0003-2697(67)90298-9. [DOI] [PubMed] [Google Scholar]
  14. Sela I. Tobacco enzyme-cleaved fragments of TMV-RNA specifically accepting serine and methionine. Virology. 1972 Jul;49(1):90–94. doi: 10.1016/s0042-6822(72)80009-6. [DOI] [PubMed] [Google Scholar]
  15. Shih D. S., Lane L. C., Kaesberg P. Origin of the small component of brome mosaic virus RNA. J Mol Biol. 1972 Mar 14;64(2):353–362. doi: 10.1016/0022-2836(72)90503-7. [DOI] [PubMed] [Google Scholar]
  16. Tao K. L., Hall T. C. Extensive charging of transfer ribonucleic acid by bean leaf extracts in vitro. Biochem J. 1971 Dec;125(4):975–981. doi: 10.1042/bj1250975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tao K. L., Hall T. C. Factors controlling aminoacyl-transfer-ribonucleic acid synthesis in vitro by a plant system. Biochem J. 1971 Feb;121(3):495–501. doi: 10.1042/bj1210495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yot P., Pinck M., Haenni A. L., Duranton H. M., Chapeville F. Valine-specific tRNA-like structure in turnip yellow mosaic virus RNA. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1345–1352. doi: 10.1073/pnas.67.3.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]

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