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. 1971 May;122(4):569–573. doi: 10.1042/bj1220569

A requirement for dietary lipids for induction of cytochrome P-450 by phenobarbitone in rat liver microsomal fraction

W J Marshall 1,*, A E M McLean 1
PMCID: PMC1176815  PMID: 5123890

Abstract

1. Rats fed on purified synthetic diets have a markedly lower cytochrome P-450 concentration and hydroxylating enzyme activity in liver microsomal fraction than rats fed on stock pellets. 2. When both groups are treated with phenobarbitone the difference is even greater, the purified diet allowing only 50% of the cytochrome P-450 concentrations of controls. 3. Addition of herring oil, linoleic acid or 0.1% oxidized sitosterol to the diets allows induction of cytochrome P-450 to take place. 4. Addition of coconut oil to the diet does not permit induction of cytochrome P-450. 5. The interactions between dietary protein and the lipid substances are explored. 6. The mechanism of induction of microsomal hydroxylation enzymes by drugs is discussed in the light of these requirements.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWN R. R., MILLER J. A., MILLER E. C. The metabolism of methylated aminoazo dyes. IV. Dietary factors enhancing demethylation in vitro. J Biol Chem. 1954 Jul;209(1):211–222. [PubMed] [Google Scholar]
  2. Bretscher M. S. How repressor molecules function. Nature. 1968 Feb 10;217(5128):509–511. doi: 10.1038/217509a0. [DOI] [PubMed] [Google Scholar]
  3. Conney A. H. Pharmacological implications of microsomal enzyme induction. Pharmacol Rev. 1967 Sep;19(3):317–366. [PubMed] [Google Scholar]
  4. KATO R., CHIESARA E., VASSANELLI P. Factors influencing induction of hepatic microsomal drug-metabolizing enzymes. Biochem Pharmacol. 1962 Mar;11:211–220. doi: 10.1016/0006-2952(62)90076-x. [DOI] [PubMed] [Google Scholar]
  5. Marshall W. J., McLean A. E. The effect of nutrition and hormonal status on cytochrome P-450 and its induction. Biochem J. 1969 Dec;115(5):27P–28P. doi: 10.1042/bj1150027p. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Marshall W. J., McLean A. E. The effect of oral phenobarbitone on hepatic microsomal cytochrome P-450 and demethylation activity in rats fed normal and low protein diets. Biochem Pharmacol. 1969 Jan;18(1):153–157. doi: 10.1016/0006-2952(69)90020-3. [DOI] [PubMed] [Google Scholar]
  7. McLean A. E., McLean E. K. The effect of diet and 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) on microsomal hydroxylating enzymes and on sensitivity of rats to carbon tetrachloride poisoning. Biochem J. 1966 Aug;100(2):564–571. doi: 10.1042/bj1000564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McLean A. E., McLean E. Diet and toxicity. Br Med Bull. 1969 Sep;25(3):278–281. doi: 10.1093/oxfordjournals.bmb.a070718. [DOI] [PubMed] [Google Scholar]
  9. Nebert D. W., Gelboin H. V. The role of ribonucleic acid and protein synthesis in microsomal aryl hydrocarbon hydroxylase induction in cell culture. The independence of transcription and translation. J Biol Chem. 1970 Jan 10;245(1):160–168. [PubMed] [Google Scholar]
  10. OMURA T., SATO R. THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE. J Biol Chem. 1964 Jul;239:2370–2378. [PubMed] [Google Scholar]

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