Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1971 Jun;123(1):69–74. doi: 10.1042/bj1230069

Isolation and partial characterization of methylated arginines from the encephalitogenic basic protein of myelin

G S Baldwin 1, P R Carnegie 1,*
PMCID: PMC1176900  PMID: 5128665

Abstract

Two methylated derivatives of arginine were isolated from the encephalitogenic protein of myelin from the central nervous system. Evidence is presented for the proposed structures, ω-NN′-dimethylarginine and ω-N-monomethylarginine. In the encephalitogenic protein from human brain the proportion 1:6:10 for arginine:monomethylarginine:dimethylarginine residues was found to occur at position 107. Possible roles for the methylated arginine in conformational changes and altered ion-exchange behaviour are discussed.

Full text

PDF
73

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMBLER R. P., REES M. W. Epsilon-N-Methyl-lysine in bacterial flagellar protein. Nature. 1959 Jul 4;184:56–57. doi: 10.1038/184056b0. [DOI] [PubMed] [Google Scholar]
  2. Asatoor A. M., Armstrong M. D. 3-methylhistidine, a component of actin. Biochem Biophys Res Commun. 1967 Jan 23;26(2):168–174. doi: 10.1016/0006-291x(67)90229-x. [DOI] [PubMed] [Google Scholar]
  3. Baldwin G. S., Carnegie P. R. Specific enzymic methylation of an arginine in the experimental allergic encephalomyelitis protein from human myelin. Science. 1971 Feb 12;171(3971):579–581. doi: 10.1126/science.171.3971.579. [DOI] [PubMed] [Google Scholar]
  4. CARNEGIE P. R., SYNGE R. L. Filter-paper ionophoresis of cupric complexes of neutral amino acids and oligopeptides. Biochem J. 1961 Apr;78:692–696. doi: 10.1042/bj0780692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carnegie P. R. Amino acid sequence of the encephalitogenic basic protein from human myelin. Biochem J. 1971 Jun;123(1):57–67. doi: 10.1042/bj1230057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carnegie P. R., Bencina B., Lamoureux G. Experimental allergic encephalomyelitis. Isolation of basic proteins and polypeptides from central nervous tissue. Biochem J. 1967 Nov;105(2):559–568. doi: 10.1042/bj1050559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DeLange R. J., Glazer A. N., Smith E. L. Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora. J Biol Chem. 1969 Mar 10;244(5):1385–1388. [PubMed] [Google Scholar]
  8. Eylar E. H. Amino acid sequence of the basic protein of the myelin membrane. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1425–1431. doi: 10.1073/pnas.67.3.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hatano H., Sumizu K., Rokushika S., Murakami F. Automatic liquid chromatography of primary mono- and diamines on a cation-exchange resin. Anal Biochem. 1970 Jun;35(2):377–383. doi: 10.1016/0003-2697(70)90198-3. [DOI] [PubMed] [Google Scholar]
  10. Kaye A. M., Sheratzky D. Methylation of protein (histone) in vitro: enzymic activity from the soluble fraction of rat organs. Biochim Biophys Acta. 1969 Oct 22;190(2):527–538. doi: 10.1016/0005-2787(69)90101-4. [DOI] [PubMed] [Google Scholar]
  11. Martenson R. E., Deibler G. E., Kies M. W. Microheterogeneity of guinea pig myelin basic protein. J Biol Chem. 1969 Aug 25;244(16):4261–4267. [PubMed] [Google Scholar]
  12. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  13. Paik W. K., Kim S. Omega-N-methylarginine in protein. J Biol Chem. 1970 Jan 10;245(1):88–92. [PubMed] [Google Scholar]
  14. Paik W. K., Kim S. Protein methylase I. Purification and properties of the enzyme. J Biol Chem. 1968 May 10;243(9):2108–2114. [PubMed] [Google Scholar]
  15. Paik W. K., Kim S. Protein methylation in rat brain in vitro. J Neurochem. 1969 Aug;16(8):1257–1261. doi: 10.1111/j.1471-4159.1969.tb05974.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES