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. 1971 Jun;123(1):97–104. doi: 10.1042/bj1230097

The catabolism of phosphatidylethanolamine by the rumen protozoon Entodinium caudatum and its conversion into the N-(1-carboxyethyl) derivative

G S Coleman 1, P Kemp 1, R M C Dawson 1
PMCID: PMC1176903  PMID: 5001897

Abstract

1. The N-(2-hydroxyethyl)alanine esterified to phosphatidic acid in anaerobic ciliate rumen protozoa has the l configuration. 2. Labelling experiments with Entodinium caudatum cultures using [32P]Pi [2-14C]ethanolamine and 32P- and 14C-labelled phosphatidylethanolamine show that phosphatidylethanolamine is the direct lipid precursor of the N-(2-hydroxyethyl)alanine-containing phospholipid. 3. Labelling experiments with [14C]starch, [14C]lactate and [14C]pyruvate with E. caudatum cultures indicate that a three-carbon glycolytic intermediate is probably the precursor of the N-(1-carboxyethyl) grouping which substitutes on the amino group of phosphatidylethanolamine. 4. [32P]phosphatidylethanolamine is catabolized by E. caudatum forming initially glycerylphosphorylethanolamine and subsequently glycerophosphate and Pi. A little phosphorylethanolamine formed may possibly arise from bacterial enzymes ingested by the protozoa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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