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. 1971 Jun;123(2):175–181. doi: 10.1042/bj1230175

Evidence for ethylation of rat liver deoxyribonucleic acid after administration of ethionine

P F Swann 1, A E Pegg 1, A Hawks 1, E Farber 1,*, P N Magee 1
PMCID: PMC1176921  PMID: 5127333

Abstract

1. Administration of a large dose (500mg/kg body wt.) of 3H-labelled l-ethionine to rats resulted in the incorporation of a small amount of radioactivity into the liver DNA. Considerable evidence that this radioactivity was not due to contamination of the isolated DNA with labelled protein, RNA, S-adenosyl-l-ethionine or l-ethionine was obtained. 2. After acidic hydrolysis of the DNA isolated from the livers of rats treated with labelled l-ethionine, virtually all of the radioactivity present in the DNA was found in a fraction with similar chromatographic properties to 7-ethylguanine. 3. Treatment of rats with comparable doses of l-methionine did not lead to the formation of 7-methylguanine in the liver DNA. 4. These results are discussed in relation to the induction of liver tumours by ethionine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burdon R. H., Adams R. L. The in vivo methylation of DNA in mouse fibroblasts. Biochim Biophys Acta. 1969 Jan 21;174(1):322–329. doi: 10.1016/0005-2787(69)90257-3. [DOI] [PubMed] [Google Scholar]
  2. Craddock V. M., Villa-Trevino S., Magee P. N. Occurrence of 7-methylguanine in nucleic acids of rat liver. Biochem J. 1968 Mar;107(2):179–184. doi: 10.1042/bj1070179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Druckrey H., Preussmann R., Ivankovic S., Schmähl D. Organotrope carcinogene Wirkungen bei 65 verschiedenen N-Nitroso-Verbindungen an BD-Ratten. Z Krebsforsch. 1967;69(2):103–201. [PubMed] [Google Scholar]
  4. FARBER E. ETHIONINE CARCINOGENESIS. Adv Cancer Res. 1963;7:383–474. doi: 10.1016/s0065-230x(08)60986-0. [DOI] [PubMed] [Google Scholar]
  5. FARBER E., SHULL K. H., VILLA-TREVINO S., LOMBARDI B., THOMAS M. BIOCHEMICAL PATHOLOGY OF ACUTE HEPATIC ADENOSINETRIPHOSPHATE DEFICIENCY. Nature. 1964 Jul 4;203:34–40. doi: 10.1038/203034a0. [DOI] [PubMed] [Google Scholar]
  6. Farber E. Ethionine fatty liver. Adv Lipid Res. 1967;5:119–183. [PubMed] [Google Scholar]
  7. Farber E., McConomy J., Franzen B., Marroquin F., Stewart G. A., Magee P. N. Interaction between ethionine and rat liver ribonucleic acid and protein in vivo. Cancer Res. 1967 Oct;27(10):1761–1772. [PubMed] [Google Scholar]
  8. Friedman M., Shull K. H., Farber E. Highly selective in vivo ethylation of rat liver nuclear protein by ethionine. Biochem Biophys Res Commun. 1969 Mar 31;34(6):857–864. doi: 10.1016/0006-291x(69)90259-9. [DOI] [PubMed] [Google Scholar]
  9. GOLD M., HURWITZ J. THE ENZYMATIC METHYLATION OF RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID. V. PURIFICATION AND PROPERTIES OF THE DEOXYRIBONUCLEIC ACID-METHYLATING ACTIVITY OF ESCHERICHIA COLI. J Biol Chem. 1964 Nov;239:3858–3865. [PubMed] [Google Scholar]
  10. Hancock R. L. Soluble RNA ethylase activity of normal and neoplastic mouse tissues. Cancer Res. 1968 Jul;28(7):1223–1230. [PubMed] [Google Scholar]
  11. Kalousek F., Morris N. R. The purification and properties of deoxyribonucleic acid methylase from rat spleen. J Biol Chem. 1969 Mar 10;244(5):1157–1163. [PubMed] [Google Scholar]
  12. Kaye A. M., Fridlender B., Salomon R., Bar-Meir S. Methylation of DNA in vitro: enzymic activity from different bacterial strains on DNA from various sources. Biochim Biophys Acta. 1967 Jul 18;142(2):331–344. doi: 10.1016/0005-2787(67)90616-8. [DOI] [PubMed] [Google Scholar]
  13. Loveless A. Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides. Nature. 1969 Jul 12;223(5202):206–207. doi: 10.1038/223206a0. [DOI] [PubMed] [Google Scholar]
  14. Ludlum D. B. Alkylated polycytidylic acid templates for RNA polymerase. Biochim Biophys Acta. 1970 Jul 16;213(1):142–148. doi: 10.1016/0005-2787(70)90015-8. [DOI] [PubMed] [Google Scholar]
  15. Ludlum D. B. The properties of 7-methylguanine-containing templates for ribonucleic acid polymerase. J Biol Chem. 1970 Feb 10;245(3):477–482. [PubMed] [Google Scholar]
  16. Magee P. N., Barnes J. M. Carcinogenic nitroso compounds. Adv Cancer Res. 1967;10:163–246. doi: 10.1016/s0065-230x(08)60079-2. [DOI] [PubMed] [Google Scholar]
  17. NATORI Y. Studies on ethionine. VI. Sex-dependent behavior of methionine and ethionine in rats. J Biol Chem. 1963 Jun;238:2075–2080. [PubMed] [Google Scholar]
  18. PARKS L. W., SCHLENK F. The stability and hydrolysis of S-adenosylmethionine; isolation of S-ribosylmethionine. J Biol Chem. 1958 Jan;230(1):295–305. [PubMed] [Google Scholar]
  19. Pegg A. E., Williams-Ashman H. G. Enzymic synthesis of spermine in rat prostate. Arch Biochem Biophys. 1970 Mar;137(1):156–165. doi: 10.1016/0003-9861(70)90422-4. [DOI] [PubMed] [Google Scholar]
  20. Shank R. C., Magee P. N. Similarities between the biochemical actions of cycasin and dimethylnitrosamine. Biochem J. 1967 Nov;105(2):521–527. doi: 10.1042/bj1050521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shull K. H., McConomy J., Vogt M., Castillo A., Farber E. On the mechanism of induction of hepatic adenosine triphosphate deficiency by ethionine. J Biol Chem. 1966 Nov 10;241(21):5060–5070. [PubMed] [Google Scholar]
  22. Smith R. C., Salmon W. D. Formation of S-adenosylethionine by ethionine-treated rats. Arch Biochem Biophys. 1965 Jul;111(1):191–196. doi: 10.1016/0003-9861(65)90339-5. [DOI] [PubMed] [Google Scholar]
  23. Swann P. F., Magee P. N. Nitrosamine-induced carcinogenesis. The alklylation of nucleic acids of the rat by N-methyl-N-nitrosourea, dimethylnitrosamine, dimethyl sulphate and methyl methanesulphonate. Biochem J. 1968 Nov;110(1):39–47. doi: 10.1042/bj1100039. [DOI] [PMC free article] [PubMed] [Google Scholar]

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